T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifs

Detalhes bibliográficos
Autor(a) principal: Melo, Andréa Barbosa de
Data de Publicação: 2013
Outros Autores: Nascimento, Eduardo J. M., Braga-Neto, Ulisses, Dhalia, Rafael, Silva, Ana Maria, Oelke, Mathias, Schneck, Jonathan P., Sidney, John, Sette, Alessandro, Montenegro, Silvia M. L., Marques, Ernesto T. A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/23667
Resumo: Este trabalho foi apoiado pela Subvenção AI-40085 dos Institutos Nacionais de Saúde; Fundação Oswaldo Cruz, PDTIS- RVR9; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação Nacional da Ciência, CCF-0845407; e o Conselho Brasileiro de Ensino Superior (CAPES), bolsa de doutorado da AB). Os financiadores não tiveram papel em design de estudo, coleta e análise de dados, decisão de publicação ou preparação do manuscrito.
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spelling Melo, Andréa Barbosa deNascimento, Eduardo J. M.Braga-Neto, UlissesDhalia, RafaelSilva, Ana MariaOelke, MathiasSchneck, Jonathan P.Sidney, JohnSette, AlessandroMontenegro, Silvia M. L.Marques, Ernesto T. A.2017-12-15T13:01:30Z2017-12-15T13:01:30Z2013MELO, A. B. de et al. T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifs. PLoS neglected tropical diseases, v. 7, n. 1, p. e1938, 2013.1935-2735https://www.arca.fiocruz.br/handle/icict/2366710.1371/journal.pntd.0001938Este trabalho foi apoiado pela Subvenção AI-40085 dos Institutos Nacionais de Saúde; Fundação Oswaldo Cruz, PDTIS- RVR9; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação Nacional da Ciência, CCF-0845407; e o Conselho Brasileiro de Ensino Superior (CAPES), bolsa de doutorado da AB). Os financiadores não tiveram papel em design de estudo, coleta e análise de dados, decisão de publicação ou preparação do manuscrito.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil.University of Pittsburgh. Center for Vaccine Research. Department of Infectious Diseases and Microbiology. Pittsburgh, Pennsylvania, United States of America.Texas A&M University. College Station. Department of Electrical and Computer Engineering. Texas, United States of America.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil.The Johns Hopkins University School of Medicine. Department of Pathology. Baltimore, Maryland, United States of America.The Johns Hopkins University School of Medicine. Department of Pathology. Baltimore, Maryland, United States of America.La Jolla Institute for Allergy and Immunology. Vaccine Discovery. La Jolla, California, United States of America.La Jolla Institute for Allergy and Immunology. Vaccine Discovery. La Jolla, California, United States of America.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Departamento de imunologia. Recife, PE, Brasil.Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Laboratório de Virologia e Terapia Experimental. Recife, PE, Brasil / University of Pittsburgh. Center for Vaccine Research. Department of Infectious Diseases and Microbiology. Pittsburgh, Pennsylvania, United States of America.The yellow fever vaccines (YF-17D-204 and 17DD) are considered to be among the safest vaccines and the presence of neutralizing antibodies is correlated with protection, although other immune effector mechanisms are known to be involved. T-cell responses are known to play an important role modulating antibody production and the killing of infected cells. However, little is known about the repertoire of T-cell responses elicited by the YF-17DD vaccine in humans. In this report, a library of 653 partially overlapping 15-mer peptides covering the envelope (Env) and nonstructural (NS) proteins 1 to 5 of the vaccine was utilized to perform a comprehensive analysis of the virus-specific CD4(+) and CD8(+) T-cell responses. The T-cell responses were screened ex-vivo by IFN-γ ELISPOT assays using blood samples from 220 YF-17DD vaccinees collected two months to four years after immunization. Each peptide was tested in 75 to 208 separate individuals of the cohort. The screening identified sixteen immunodominant antigens that elicited activation of circulating memory T-cells in 10% to 33% of the individuals. Biochemical in-vitro binding assays and immunogenetic and immunogenicity studies indicated that each of the sixteen immunogenic 15-mer peptides contained two or more partially overlapping epitopes that could bind with high affinity to molecules of different HLAs. The prevalence of the immunogenicity of a peptide in the cohort was correlated with the diversity of HLA-II alleles that they could bind. These findings suggest that overlapping of HLA binding motifs within a peptide enhances its T-cell immunogenicity and the prevalence of the response in the population. In summary, the results suggests that in addition to factors of the innate immunity, "promiscuous" T-cell antigens might contribute to the high efficacy of the yellow fever vaccines.engAlan L. RothmanFebre AmarelacitologiaVacinasCD4yellow fevercytologyVaccineCD4Epitopos de Linfócito TimunologiaAntígenos de Histocompatibilidade Classe IIimunologiaAntígenos de Histocompatibilidade Classe IimunologiaMemória ImunológicaHumanosLinfócitos TimunologiaVacina contra Febre AmarelaimunologiaInterferon gamasecreçãoVacina contra Febre Amarelaadministração & dosagemELISPOTT-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83092https://www.arca.fiocruz.br/bitstream/icict/23667/1/license.txt447f2497af1ef5cf99b5c07f6fa18dceMD51ORIGINALart. T-Cell Memory - melo.pdfart. 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dc.title.pt_BR.fl_str_mv T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifs
title T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifs
spellingShingle T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifs
Melo, Andréa Barbosa de
Febre Amarela
citologia
Vacinas
CD4
yellow fever
cytology
Vaccine
CD4
Epitopos de Linfócito T
imunologia
Antígenos de Histocompatibilidade Classe II
imunologia
Antígenos de Histocompatibilidade Classe I
imunologia
Memória Imunológica
Humanos
Linfócitos T
imunologia
Vacina contra Febre Amarela
imunologia
Interferon gama
secreção
Vacina contra Febre Amarela
administração & dosagem
ELISPOT
title_short T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifs
title_full T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifs
title_fullStr T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifs
title_full_unstemmed T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifs
title_sort T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifs
author Melo, Andréa Barbosa de
author_facet Melo, Andréa Barbosa de
Nascimento, Eduardo J. M.
Braga-Neto, Ulisses
Dhalia, Rafael
Silva, Ana Maria
Oelke, Mathias
Schneck, Jonathan P.
Sidney, John
Sette, Alessandro
Montenegro, Silvia M. L.
Marques, Ernesto T. A.
author_role author
author2 Nascimento, Eduardo J. M.
Braga-Neto, Ulisses
Dhalia, Rafael
Silva, Ana Maria
Oelke, Mathias
Schneck, Jonathan P.
Sidney, John
Sette, Alessandro
Montenegro, Silvia M. L.
Marques, Ernesto T. A.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Melo, Andréa Barbosa de
Nascimento, Eduardo J. M.
Braga-Neto, Ulisses
Dhalia, Rafael
Silva, Ana Maria
Oelke, Mathias
Schneck, Jonathan P.
Sidney, John
Sette, Alessandro
Montenegro, Silvia M. L.
Marques, Ernesto T. A.
dc.subject.other.pt_BR.fl_str_mv Febre Amarela
citologia
Vacinas
CD4
topic Febre Amarela
citologia
Vacinas
CD4
yellow fever
cytology
Vaccine
CD4
Epitopos de Linfócito T
imunologia
Antígenos de Histocompatibilidade Classe II
imunologia
Antígenos de Histocompatibilidade Classe I
imunologia
Memória Imunológica
Humanos
Linfócitos T
imunologia
Vacina contra Febre Amarela
imunologia
Interferon gama
secreção
Vacina contra Febre Amarela
administração & dosagem
ELISPOT
dc.subject.en.pt_BR.fl_str_mv yellow fever
cytology
Vaccine
CD4
dc.subject.decs.pt_BR.fl_str_mv Epitopos de Linfócito T
imunologia
Antígenos de Histocompatibilidade Classe II
imunologia
Antígenos de Histocompatibilidade Classe I
imunologia
Memória Imunológica
Humanos
Linfócitos T
imunologia
Vacina contra Febre Amarela
imunologia
Interferon gama
secreção
Vacina contra Febre Amarela
administração & dosagem
ELISPOT
description Este trabalho foi apoiado pela Subvenção AI-40085 dos Institutos Nacionais de Saúde; Fundação Oswaldo Cruz, PDTIS- RVR9; Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq); Fundação Nacional da Ciência, CCF-0845407; e o Conselho Brasileiro de Ensino Superior (CAPES), bolsa de doutorado da AB). Os financiadores não tiveram papel em design de estudo, coleta e análise de dados, decisão de publicação ou preparação do manuscrito.
publishDate 2013
dc.date.issued.fl_str_mv 2013
dc.date.accessioned.fl_str_mv 2017-12-15T13:01:30Z
dc.date.available.fl_str_mv 2017-12-15T13:01:30Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MELO, A. B. de et al. T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifs. PLoS neglected tropical diseases, v. 7, n. 1, p. e1938, 2013.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/23667
dc.identifier.issn.pt_BR.fl_str_mv 1935-2735
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0001938
identifier_str_mv MELO, A. B. de et al. T-cell memory responses elicited by yellow fever vaccine are targeted to overlapping epitopes containing multiple HLA-I and -II binding motifs. PLoS neglected tropical diseases, v. 7, n. 1, p. e1938, 2013.
1935-2735
10.1371/journal.pntd.0001938
url https://www.arca.fiocruz.br/handle/icict/23667
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Alan L. Rothman
publisher.none.fl_str_mv Alan L. Rothman
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/23667/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/23667/2/art.%20T-Cell%20Memory%20-%20melo.pdf
https://www.arca.fiocruz.br/bitstream/icict/23667/3/art.%20T-Cell%20Memory%20-%20melo.pdf.txt
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