Molecular Studies of the Brazilian Infectious Bronchitis Virus Isolates
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2010 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/4183 |
Resumo: | Pontifícia Universidade Católica de Minas Gerais. Escola de Veterinária. Betim, MG, Brasil. |
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Abreu, Josiane TavaresMourão, Marina de MoraesSantos, C. E.Veloso, Caio Julio MartinsResende, J. S.Flatschart, R. B.Flatschart, A. V. FolguerasJúnior, S. N.Santoro, Marcelo MatosMendes, A. C. R.Franco, Glória ReginaSilva, A.Campos, A. B.Fernandez, S.2012-07-18T16:18:18Z2012-07-18T16:18:18Z2010ABREU, Josiane Tavares et al . Molecular studies of the Brazilian infectious bronchitis virus isolates. Rev. Bras. Cienc. Avic., 2010, v. 12, n. 2, p. 107-110, 2010.1516-635Xhttps://www.arca.fiocruz.br/handle/icict/4183eng122Molecular Studies of the Brazilian Infectious Bronchitis Virus Isolatesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRevista Brasileira de Ciência AvícolaPontifícia Universidade Católica de Minas Gerais. Escola de Veterinária. Betim, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Belo Horizonte, MG, Brazil / Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores e Monitoração. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil.Pontifícia Universidade Católica de Minas Gerais. Escola de Veterinária. Betim, MG, Brasil.Universidade Federal de Minas Gerais . Escola de Veterinária. Departamento de Medicina Veterinária Preventiva. Belo Horizonte, MG, Brazil.INMETRO. Duque de Caxias, RJ, Brazil.EMBRAPA Milho e Sorgo. Sete Lagoas, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil.Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil.Pontifícia Universidade Católica de Minas Gerais. Escola de Veterinária. Betim, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciencias Biologicas. Belo Horizonte, MG, Brazil.Laboratório Bio-Vet S.A. Vargem Grande Paulista, SP, Brazil.Laboratório Bio-Vet S.A. Vargem Grande Paulista, SP, Brazil.Laboratório Bio-Vet S.A. Vargem Grande Paulista, SP, Brazil.Avian infectious bronchitis virus (IBV) isolates have been widely characterized by reverse transcription followed by polymerase chain reaction and DNA sequencing. In present study, these techniques were applied to three viral genomic regions comprising the complete and/or a partial S1 segment, S2 and nucleocapsid genes. DNA sequences from viral isolates obtained from 1972 to 1989 and from 2006 to 2008 were compared. High similarity (>90%) was observed among some of the genomic segments, including S1 hypervariable region, which could suggest a common origin or ancestry. DNA sequences from S2 and N protein genes obtained from different infected tissues of the same flock were analyzed, and a clear segregation between respiratory and intestinal tract was observed. Therefore, these data suggest cocirculation of more than one viral strain in the same flock. 57.1% of DNA sequences from the S1 complete segment samples, 53.3% from the S2 fragment and 62.5% from the partial N gene were found to be different from analyzed sequences from reference strains leading tothe conclusion that parte of viral isolates included in this study may be considered region specific. Considering the simultaneous analysis of the three genes, a large IBV genetic profile was observed in both old and recent isolates groups. However, most prominent diversity between viral isolates was obtained in the period from 1972 and 1989, showing the presence of a large number of variants in the state of Minas Gerais before the official approval of vaccination (1980).Infectious bronchitis virusgenotypingphylogenetic treenucleocapsidspike glycoproteininfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINAL3-Molecular Studies of the Brazilian.pdf3-Molecular Studies of the Brazilian.pdfapplication/pdf74538https://www.arca.fiocruz.br/bitstream/icict/4183/1/3-Molecular%20Studies%20of%20the%20Brazilian.pdf13351d05f4f85b5d2d584d171b8cc0f4MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81990https://www.arca.fiocruz.br/bitstream/icict/4183/2/license.txtb6ead4607e393dee1190c21ba08397beMD52TEXT3-Molecular Studies of the Brazilian.pdf.txt3-Molecular Studies of the Brazilian.pdf.txtExtracted texttext/plain19429https://www.arca.fiocruz.br/bitstream/icict/4183/5/3-Molecular%20Studies%20of%20the%20Brazilian.pdf.txtd44c65a94e649996553b0f33f05ade94MD55THUMBNAIL3-Molecular Studies of the Brazilian.pdf.jpg3-Molecular Studies of the Brazilian.pdf.jpgGenerated Thumbnailimage/jpeg2388https://www.arca.fiocruz.br/bitstream/icict/4183/4/3-Molecular%20Studies%20of%20the%20Brazilian.pdf.jpgc9c7ad03e1f8cf6456f2b1c7ffb33a9dMD54icict/41832018-04-06 08:58:03.837oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:58:03Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Molecular Studies of the Brazilian Infectious Bronchitis Virus Isolates |
| title |
Molecular Studies of the Brazilian Infectious Bronchitis Virus Isolates |
| spellingShingle |
Molecular Studies of the Brazilian Infectious Bronchitis Virus Isolates Abreu, Josiane Tavares Infectious bronchitis virus genotyping phylogenetic tree nucleocapsid spike glycoprotein |
| title_short |
Molecular Studies of the Brazilian Infectious Bronchitis Virus Isolates |
| title_full |
Molecular Studies of the Brazilian Infectious Bronchitis Virus Isolates |
| title_fullStr |
Molecular Studies of the Brazilian Infectious Bronchitis Virus Isolates |
| title_full_unstemmed |
Molecular Studies of the Brazilian Infectious Bronchitis Virus Isolates |
| title_sort |
Molecular Studies of the Brazilian Infectious Bronchitis Virus Isolates |
| author |
Abreu, Josiane Tavares |
| author_facet |
Abreu, Josiane Tavares Mourão, Marina de Moraes Santos, C. E. Veloso, Caio Julio Martins Resende, J. S. Flatschart, R. B. Flatschart, A. V. Folgueras Júnior, S. N. Santoro, Marcelo Matos Mendes, A. C. R. Franco, Glória Regina Silva, A. Campos, A. B. Fernandez, S. |
| author_role |
author |
| author2 |
Mourão, Marina de Moraes Santos, C. E. Veloso, Caio Julio Martins Resende, J. S. Flatschart, R. B. Flatschart, A. V. Folgueras Júnior, S. N. Santoro, Marcelo Matos Mendes, A. C. R. Franco, Glória Regina Silva, A. Campos, A. B. Fernandez, S. |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Abreu, Josiane Tavares Mourão, Marina de Moraes Santos, C. E. Veloso, Caio Julio Martins Resende, J. S. Flatschart, R. B. Flatschart, A. V. Folgueras Júnior, S. N. Santoro, Marcelo Matos Mendes, A. C. R. Franco, Glória Regina Silva, A. Campos, A. B. Fernandez, S. |
| dc.subject.en.pt_BR.fl_str_mv |
Infectious bronchitis virus genotyping phylogenetic tree nucleocapsid spike glycoprotein |
| topic |
Infectious bronchitis virus genotyping phylogenetic tree nucleocapsid spike glycoprotein |
| description |
Pontifícia Universidade Católica de Minas Gerais. Escola de Veterinária. Betim, MG, Brasil. |
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2010 |
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2010 |
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2012-07-18T16:18:18Z |
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ABREU, Josiane Tavares et al . Molecular studies of the Brazilian infectious bronchitis virus isolates. Rev. Bras. Cienc. Avic., 2010, v. 12, n. 2, p. 107-110, 2010. |
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1516-635X |
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ABREU, Josiane Tavares et al . Molecular studies of the Brazilian infectious bronchitis virus isolates. Rev. Bras. Cienc. Avic., 2010, v. 12, n. 2, p. 107-110, 2010. 1516-635X |
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