Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles.

Detalhes bibliográficos
Autor(a) principal: OKINO, C. H.
Data de Publicação: 2017
Outros Autores: MORES, M. A. Z., TREVISOL, I. M., COLDEBELLA, A., MONTASSIER, H. J., BRENTANO, L.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
Texto Completo: http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094609
Resumo: Avian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related genes induced by challenge with the IBV B isolate when compared to the challenge with to the IBV A isolate. Cell-mediated immune (CMI) related genes presented also lower levels of expression in tracheal samples from birds challenged with B isolate at 1dpi. Increased viral load and a higher percentage of birds with relevant lesions were observed in both tracheal and renal samples from chickens exposed to challenge with IBV B isolate. This differential pattern of early immune responses developed after challenge with IBV B isolate, related to the downregulation of TLR7, leading to insufficient pro-inflammatory response and lower CMI responses, seem to have an association with a most severe renal lesion and an enhanced capability of replication of this isolate in chicken.
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spelling Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles.BronquiteFrangoBronchitisInfectious bronchitisInfectious bronchitis virusChickensAvian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related genes induced by challenge with the IBV B isolate when compared to the challenge with to the IBV A isolate. Cell-mediated immune (CMI) related genes presented also lower levels of expression in tracheal samples from birds challenged with B isolate at 1dpi. Increased viral load and a higher percentage of birds with relevant lesions were observed in both tracheal and renal samples from chickens exposed to challenge with IBV B isolate. This differential pattern of early immune responses developed after challenge with IBV B isolate, related to the downregulation of TLR7, leading to insufficient pro-inflammatory response and lower CMI responses, seem to have an association with a most severe renal lesion and an enhanced capability of replication of this isolate in chicken.CINTIA HIROMI OKINO, CPPSE; MARCOS ANTONIO ZANELLA MORES, CNPSA; IARA MARIA TREVISOL, CNPSA; ARLEI COLDEBELLA, CNPSA; Hélio José Montassier, UNESP; LIANA BRENTANO, CNPSA.OKINO, C. H.MORES, M. A. Z.TREVISOL, I. M.COLDEBELLA, A.MONTASSIER, H. J.BRENTANO, L.2019-05-22T01:11:33Z2019-05-22T01:11:33Z2018-08-2820172019-05-22T01:11:33Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-17Plos One, v. 12, p. 1-17, fev. 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094609enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-05-22T01:11:41Zoai:www.alice.cnptia.embrapa.br:doc/1094609Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-05-22T01:11:41falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-05-22T01:11:41Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false
dc.title.none.fl_str_mv Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles.
title Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles.
spellingShingle Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles.
OKINO, C. H.
Bronquite
Frango
Bronchitis
Infectious bronchitis
Infectious bronchitis virus
Chickens
title_short Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles.
title_full Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles.
title_fullStr Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles.
title_full_unstemmed Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles.
title_sort Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles.
author OKINO, C. H.
author_facet OKINO, C. H.
MORES, M. A. Z.
TREVISOL, I. M.
COLDEBELLA, A.
MONTASSIER, H. J.
BRENTANO, L.
author_role author
author2 MORES, M. A. Z.
TREVISOL, I. M.
COLDEBELLA, A.
MONTASSIER, H. J.
BRENTANO, L.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv CINTIA HIROMI OKINO, CPPSE; MARCOS ANTONIO ZANELLA MORES, CNPSA; IARA MARIA TREVISOL, CNPSA; ARLEI COLDEBELLA, CNPSA; Hélio José Montassier, UNESP; LIANA BRENTANO, CNPSA.
dc.contributor.author.fl_str_mv OKINO, C. H.
MORES, M. A. Z.
TREVISOL, I. M.
COLDEBELLA, A.
MONTASSIER, H. J.
BRENTANO, L.
dc.subject.por.fl_str_mv Bronquite
Frango
Bronchitis
Infectious bronchitis
Infectious bronchitis virus
Chickens
topic Bronquite
Frango
Bronchitis
Infectious bronchitis
Infectious bronchitis virus
Chickens
description Avian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related genes induced by challenge with the IBV B isolate when compared to the challenge with to the IBV A isolate. Cell-mediated immune (CMI) related genes presented also lower levels of expression in tracheal samples from birds challenged with B isolate at 1dpi. Increased viral load and a higher percentage of birds with relevant lesions were observed in both tracheal and renal samples from chickens exposed to challenge with IBV B isolate. This differential pattern of early immune responses developed after challenge with IBV B isolate, related to the downregulation of TLR7, leading to insufficient pro-inflammatory response and lower CMI responses, seem to have an association with a most severe renal lesion and an enhanced capability of replication of this isolate in chicken.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018-08-28
2019-05-22T01:11:33Z
2019-05-22T01:11:33Z
2019-05-22T01:11:33Z
dc.type.driver.fl_str_mv info:eu-repo/semantics/publishedVersion
info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv Plos One, v. 12, p. 1-17, fev. 2017.
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094609
identifier_str_mv Plos One, v. 12, p. 1-17, fev. 2017.
url http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094609
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 1-17
dc.source.none.fl_str_mv reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron:EMBRAPA
instname_str Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
instacron_str EMBRAPA
institution EMBRAPA
reponame_str Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
collection Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)
repository.name.fl_str_mv Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)
repository.mail.fl_str_mv cg-riaa@embrapa.br
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