Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
Texto Completo: | http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094609 |
Resumo: | Avian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related genes induced by challenge with the IBV B isolate when compared to the challenge with to the IBV A isolate. Cell-mediated immune (CMI) related genes presented also lower levels of expression in tracheal samples from birds challenged with B isolate at 1dpi. Increased viral load and a higher percentage of birds with relevant lesions were observed in both tracheal and renal samples from chickens exposed to challenge with IBV B isolate. This differential pattern of early immune responses developed after challenge with IBV B isolate, related to the downregulation of TLR7, leading to insufficient pro-inflammatory response and lower CMI responses, seem to have an association with a most severe renal lesion and an enhanced capability of replication of this isolate in chicken. |
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Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles.BronquiteFrangoBronchitisInfectious bronchitisInfectious bronchitis virusChickensAvian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related genes induced by challenge with the IBV B isolate when compared to the challenge with to the IBV A isolate. Cell-mediated immune (CMI) related genes presented also lower levels of expression in tracheal samples from birds challenged with B isolate at 1dpi. Increased viral load and a higher percentage of birds with relevant lesions were observed in both tracheal and renal samples from chickens exposed to challenge with IBV B isolate. This differential pattern of early immune responses developed after challenge with IBV B isolate, related to the downregulation of TLR7, leading to insufficient pro-inflammatory response and lower CMI responses, seem to have an association with a most severe renal lesion and an enhanced capability of replication of this isolate in chicken.CINTIA HIROMI OKINO, CPPSE; MARCOS ANTONIO ZANELLA MORES, CNPSA; IARA MARIA TREVISOL, CNPSA; ARLEI COLDEBELLA, CNPSA; Hélio José Montassier, UNESP; LIANA BRENTANO, CNPSA.OKINO, C. H.MORES, M. A. Z.TREVISOL, I. M.COLDEBELLA, A.MONTASSIER, H. J.BRENTANO, L.2019-05-22T01:11:33Z2019-05-22T01:11:33Z2018-08-2820172019-05-22T01:11:33Zinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article1-17Plos One, v. 12, p. 1-17, fev. 2017.http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094609enginfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice)instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa)instacron:EMBRAPA2019-05-22T01:11:41Zoai:www.alice.cnptia.embrapa.br:doc/1094609Repositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestopendoar:21542019-05-22T01:11:41falseRepositório InstitucionalPUBhttps://www.alice.cnptia.embrapa.br/oai/requestcg-riaa@embrapa.bropendoar:21542019-05-22T01:11:41Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa)false |
dc.title.none.fl_str_mv |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles. |
title |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles. |
spellingShingle |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles. OKINO, C. H. Bronquite Frango Bronchitis Infectious bronchitis Infectious bronchitis virus Chickens |
title_short |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles. |
title_full |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles. |
title_fullStr |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles. |
title_full_unstemmed |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles. |
title_sort |
Early immune responses and development of pathogenesis of avian infectious bronchitis viruses with different virulence profiles. |
author |
OKINO, C. H. |
author_facet |
OKINO, C. H. MORES, M. A. Z. TREVISOL, I. M. COLDEBELLA, A. MONTASSIER, H. J. BRENTANO, L. |
author_role |
author |
author2 |
MORES, M. A. Z. TREVISOL, I. M. COLDEBELLA, A. MONTASSIER, H. J. BRENTANO, L. |
author2_role |
author author author author author |
dc.contributor.none.fl_str_mv |
CINTIA HIROMI OKINO, CPPSE; MARCOS ANTONIO ZANELLA MORES, CNPSA; IARA MARIA TREVISOL, CNPSA; ARLEI COLDEBELLA, CNPSA; Hélio José Montassier, UNESP; LIANA BRENTANO, CNPSA. |
dc.contributor.author.fl_str_mv |
OKINO, C. H. MORES, M. A. Z. TREVISOL, I. M. COLDEBELLA, A. MONTASSIER, H. J. BRENTANO, L. |
dc.subject.por.fl_str_mv |
Bronquite Frango Bronchitis Infectious bronchitis Infectious bronchitis virus Chickens |
topic |
Bronquite Frango Bronchitis Infectious bronchitis Infectious bronchitis virus Chickens |
description |
Avian infectious bronchitis virus (IBV) primarily replicates in epithelial cells of the upper respiratory tract of chickens, inducing both morphological and immune modulatory changes. However, the association between the local immune responses induced by IBV and the mechanisms of pathogenesis has not yet been completely elucidated. This study compared the expression profile of genes related to immune responses in tracheal samples after challenge with two Brazilian field isolates (A and B) of IBV from the same genotype, associating these responses with viral replication and with pathological changes in trachea and kidney. We detected a suppressive effect on the early activation of TLR7 pathway, followed by lower expression levels of inflammatory related genes induced by challenge with the IBV B isolate when compared to the challenge with to the IBV A isolate. Cell-mediated immune (CMI) related genes presented also lower levels of expression in tracheal samples from birds challenged with B isolate at 1dpi. Increased viral load and a higher percentage of birds with relevant lesions were observed in both tracheal and renal samples from chickens exposed to challenge with IBV B isolate. This differential pattern of early immune responses developed after challenge with IBV B isolate, related to the downregulation of TLR7, leading to insufficient pro-inflammatory response and lower CMI responses, seem to have an association with a most severe renal lesion and an enhanced capability of replication of this isolate in chicken. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017 2018-08-28 2019-05-22T01:11:33Z 2019-05-22T01:11:33Z 2019-05-22T01:11:33Z |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/publishedVersion info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
Plos One, v. 12, p. 1-17, fev. 2017. http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094609 |
identifier_str_mv |
Plos One, v. 12, p. 1-17, fev. 2017. |
url |
http://www.alice.cnptia.embrapa.br/alice/handle/doc/1094609 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
1-17 |
dc.source.none.fl_str_mv |
reponame:Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) instname:Empresa Brasileira de Pesquisa Agropecuária (Embrapa) instacron:EMBRAPA |
instname_str |
Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
instacron_str |
EMBRAPA |
institution |
EMBRAPA |
reponame_str |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
collection |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) |
repository.name.fl_str_mv |
Repositório Institucional da EMBRAPA (Repository Open Access to Scientific Information from EMBRAPA - Alice) - Empresa Brasileira de Pesquisa Agropecuária (Embrapa) |
repository.mail.fl_str_mv |
cg-riaa@embrapa.br |
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1794503475521912832 |