Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/35356 |
Resumo: | NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario. Derio, Bizkaia, Spain / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil. |
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González, Mikel A.Pavan, Márcio G.Fernandes, Rosilainy S.Busquets, NúriaDavid, Mariana R.Oliveira, Ricardo LourençoGarcía-Pérez, Ana L.Freitas, Rafael Maciel de2019-09-05T15:23:14Z2019-09-05T15:23:14Z2019GONZÁLEZ, Mikel A. et al. Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain. Parasites Vectors, v. 12, n. 150, p. 1-9, 2019.1756-3305https://www.arca.fiocruz.br/handle/icict/3535610.1186/s13071-019-3359-1engBMCAedes albopictusZika vírusPopulações espanholasCompetência de vetorEnsaio de placaCópias viraisReação em Cadeia da Polimerase em Tempo RealAedes albopictusZika virusSpanish populationsVector competenceViral copiesRT-qPCRPlaque assayReação em Cadeia da Polimerase em Tempo RealLimited risk of Zika virus transmission by five Aedes albopictus populations from Spaininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleNEIKER-Instituto Vasco de Investigación y Desarrollo Agrario. Derio, Bizkaia, Spain / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Universitat Autònoma de Barcelona. IRTA, Centre de Recerca en Sanitat Animal (CReSA, IRTA-UAB), Bellaterra, Spain.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario. Derio, Bizkaia, Spain.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil / Universidade Federal do Rio de Janeiro. Instituto Nacional de Ciência e Tecnologia em Entomologia Molecular. Rio de Janeiro, RJ, Brasil.Aedes albopictus, the Asian tiger mosquito, is an exotic invasive species in Europe. It has substantial public health relevance due to its potential role in transmitting several human pathogens. Out of the European countries, Spain has one of the highest risk levels of autochthonous arbovirus transmission due to both the high density of Ae. albopictus and the extensive tourist influx from vector-endemic areas. This study aims to investigate the susceptibility of five Ae. albopictus populations from mainland Spain and the Balearic Islands to a Brazilian Zika virus (ZIKV) strain. Methods: The F1 generation of each Ae. albopictus population was orally challenged with a ZIKV-infected blood meal (1.8×106 PFU/ml). At 7 and 14 days post-infection (dpi), mosquito bodies (thorax and abdomen) and heads were individually analysed through RT-qPCR to determine the infection rate (IR) and dissemination rate (DR), respectively. The saliva of infected mosquitoes was inoculated in Vero cells and the transmission rate was assessed by plaque assay or RT-qPCR on ~33 individuals per population. Results: The IR and DR ranged between 12–88%, and 0–60%, respectively, suggesting that ZIKV is capable of crossing the midgut barrier. Remarkably, no infectious viral particle was found in saliva samples, indicating a low ability of ZIKV to overcome the salivary gland barrier. A subsequent assay revealed that a second non-infective blood meal 48 h after ZIKV exposure did not infuence Ae. albopictus vector competence. Conclusions: The oral experimental ZIKV infections performed here indicate that Ae. albopictus from Spain become infected and disseminate the virus through the body but has a limited ability to transmit the Brazilian ZIKV strain through biting. Therefore, the results suggest a limited risk of autochthonous ZIKV transmission in Spain by Ae. albopictus.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/35356/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALRafaelFreitas_MarianaDavid_etal_IOC_2019.pdfRafaelFreitas_MarianaDavid_etal_IOC_2019.pdfapplication/pdf1366827https://www.arca.fiocruz.br/bitstream/icict/35356/2/RafaelFreitas_MarianaDavid_etal_IOC_2019.pdfb8cc8d308222a6391e081f1f0e1f8bb8MD52TEXTRafaelFreitas_MarianaDavid_etal_IOC_2019.pdf.txtRafaelFreitas_MarianaDavid_etal_IOC_2019.pdf.txtExtracted texttext/plain42097https://www.arca.fiocruz.br/bitstream/icict/35356/3/RafaelFreitas_MarianaDavid_etal_IOC_2019.pdf.txt785bb33f1b21ea7b95568a1823500dc8MD53icict/353562019-09-06 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| dc.title.pt_BR.fl_str_mv |
Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain |
| title |
Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain |
| spellingShingle |
Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain González, Mikel A. Aedes albopictus Zika vírus Populações espanholas Competência de vetor Ensaio de placa Cópias virais Reação em Cadeia da Polimerase em Tempo Real Aedes albopictus Zika virus Spanish populations Vector competence Viral copies RT-qPCR Plaque assay Reação em Cadeia da Polimerase em Tempo Real |
| title_short |
Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain |
| title_full |
Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain |
| title_fullStr |
Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain |
| title_full_unstemmed |
Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain |
| title_sort |
Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain |
| author |
González, Mikel A. |
| author_facet |
González, Mikel A. Pavan, Márcio G. Fernandes, Rosilainy S. Busquets, Núria David, Mariana R. Oliveira, Ricardo Lourenço García-Pérez, Ana L. Freitas, Rafael Maciel de |
| author_role |
author |
| author2 |
Pavan, Márcio G. Fernandes, Rosilainy S. Busquets, Núria David, Mariana R. Oliveira, Ricardo Lourenço García-Pérez, Ana L. Freitas, Rafael Maciel de |
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author author author author author author author |
| dc.contributor.author.fl_str_mv |
González, Mikel A. Pavan, Márcio G. Fernandes, Rosilainy S. Busquets, Núria David, Mariana R. Oliveira, Ricardo Lourenço García-Pérez, Ana L. Freitas, Rafael Maciel de |
| dc.subject.other.pt_BR.fl_str_mv |
Aedes albopictus Zika vírus Populações espanholas Competência de vetor Ensaio de placa Cópias virais Reação em Cadeia da Polimerase em Tempo Real |
| topic |
Aedes albopictus Zika vírus Populações espanholas Competência de vetor Ensaio de placa Cópias virais Reação em Cadeia da Polimerase em Tempo Real Aedes albopictus Zika virus Spanish populations Vector competence Viral copies RT-qPCR Plaque assay Reação em Cadeia da Polimerase em Tempo Real |
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Aedes albopictus Zika virus Spanish populations Vector competence Viral copies RT-qPCR Plaque assay |
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Reação em Cadeia da Polimerase em Tempo Real |
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NEIKER-Instituto Vasco de Investigación y Desarrollo Agrario. Derio, Bizkaia, Spain / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Mosquitos Transmissores de Hematozoários. Rio de Janeiro, RJ, Brasil. |
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2019 |
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2019-09-05T15:23:14Z |
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2019-09-05T15:23:14Z |
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2019 |
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GONZÁLEZ, Mikel A. et al. Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain. Parasites Vectors, v. 12, n. 150, p. 1-9, 2019. |
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https://www.arca.fiocruz.br/handle/icict/35356 |
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1756-3305 |
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10.1186/s13071-019-3359-1 |
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GONZÁLEZ, Mikel A. et al. Limited risk of Zika virus transmission by five Aedes albopictus populations from Spain. Parasites Vectors, v. 12, n. 150, p. 1-9, 2019. 1756-3305 10.1186/s13071-019-3359-1 |
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