Zika virus infects human blood mononuclear cells
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/41050 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. |
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Messias, Carolina V.Lemos, Julia P.Cunha, Daniela P.Vasconcelos, Zilton Farias Meira deRaphael, Lidiane M. S.Bonaldo, Myrna C.Alves, Bruno CisterBou-Habib, Dumith ChequerAlmeida, Vinicius Cotta deSavino, WilsonCruz, Daniela A. Mendes da2020-04-30T18:26:39Z2020-04-30T18:26:39Z2019MESSIAS, Carolina V. et al. Zika virus infects human blood mononuclear cells. BMC Infectious Diseases, v. 19, n. 986, p. 1-6, 2019.1471-2334https://www.arca.fiocruz.br/handle/icict/4105010.1186/s12879-019-4622-yengBMC Infectious DiseaseZika virúsLinfócitosMonócitosCitometria de fluxoReação em Cadeia da Polimerase em Tempo RealZika virusLymphocytesMonocytesFlow cytometryRT-qPCRReação em Cadeia da Polimerase em Tempo RealZika virus infects human blood mononuclear cellsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Laboratório de Alta Complexidade. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Saúde da Mulher, da Criança e do Adolescente Fernandes Figueira. Laboratório de Alta Complexidade. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil.Background: Zika virus (ZIKV) infection gained public health concern after the 2015 outbreak in Brazil, when microcephaly rates increased in babies born from infected mothers. It was demonstrated that ZIKV causes a congenital Zika virus syndrome, including various alterations in the development of the central nervous system. Although the infection of cells from the nervous system has been well documented, less is known in respect of ZIKV ability to infect immune cells. Herein, we investigated if peripheral blood mononuclear cells (PBMCs), freshlyisolated from healthy donors, could be infected by ZIKV. Methods: PBMCs from healthy donors were isolated and cultured in medium with ZIKV strain Rio-U1 (MOI = 0.1). Infection was analyzed by RT-qPCR and flow cytometry. Results: We detected the ZIKV RNA in PBMCs from all donors by RT-qPCR analysis. The detection of viral antigens by flow cytometry revealed that PBMC from more than 50% the donors were infected by ZIKV, with CD3+CD4+T cells, CD3−CD19+ B cells and CD3+CD8+ T cells being, respectively, the most frequently infected subpopulations,followed by CD14+ monocytes. Additionally, we observed high variability in PBMC infection rates among different donors, either by numbers or type infected cells. Conclusions: These findings raise the hypothesis that PBMCs can act as a reservoir of the virus, which may facilitate viral dissemination to different organs, including immune-privileged sites.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/41050/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDanielaMCruz_WilsonSavino_etal_IOC_2019.pdfDanielaMCruz_WilsonSavino_etal_IOC_2019.pdfapplication/pdf574489https://www.arca.fiocruz.br/bitstream/icict/41050/2/DanielaMCruz_WilsonSavino_etal_IOC_2019.pdf0e1568dedbc42a4ab373f71b59461ad5MD52TEXTDanielaMCruz_WilsonSavino_etal_IOC_2019.pdf.txtDanielaMCruz_WilsonSavino_etal_IOC_2019.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Zika virus infects human blood mononuclear cells |
| title |
Zika virus infects human blood mononuclear cells |
| spellingShingle |
Zika virus infects human blood mononuclear cells Messias, Carolina V. Zika virús Linfócitos Monócitos Citometria de fluxo Reação em Cadeia da Polimerase em Tempo Real Zika virus Lymphocytes Monocytes Flow cytometry RT-qPCR Reação em Cadeia da Polimerase em Tempo Real |
| title_short |
Zika virus infects human blood mononuclear cells |
| title_full |
Zika virus infects human blood mononuclear cells |
| title_fullStr |
Zika virus infects human blood mononuclear cells |
| title_full_unstemmed |
Zika virus infects human blood mononuclear cells |
| title_sort |
Zika virus infects human blood mononuclear cells |
| author |
Messias, Carolina V. |
| author_facet |
Messias, Carolina V. Lemos, Julia P. Cunha, Daniela P. Vasconcelos, Zilton Farias Meira de Raphael, Lidiane M. S. Bonaldo, Myrna C. Alves, Bruno Cister Bou-Habib, Dumith Chequer Almeida, Vinicius Cotta de Savino, Wilson Cruz, Daniela A. Mendes da |
| author_role |
author |
| author2 |
Lemos, Julia P. Cunha, Daniela P. Vasconcelos, Zilton Farias Meira de Raphael, Lidiane M. S. Bonaldo, Myrna C. Alves, Bruno Cister Bou-Habib, Dumith Chequer Almeida, Vinicius Cotta de Savino, Wilson Cruz, Daniela A. Mendes da |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Messias, Carolina V. Lemos, Julia P. Cunha, Daniela P. Vasconcelos, Zilton Farias Meira de Raphael, Lidiane M. S. Bonaldo, Myrna C. Alves, Bruno Cister Bou-Habib, Dumith Chequer Almeida, Vinicius Cotta de Savino, Wilson Cruz, Daniela A. Mendes da |
| dc.subject.other.pt_BR.fl_str_mv |
Zika virús Linfócitos Monócitos Citometria de fluxo Reação em Cadeia da Polimerase em Tempo Real |
| topic |
Zika virús Linfócitos Monócitos Citometria de fluxo Reação em Cadeia da Polimerase em Tempo Real Zika virus Lymphocytes Monocytes Flow cytometry RT-qPCR Reação em Cadeia da Polimerase em Tempo Real |
| dc.subject.en.pt_BR.fl_str_mv |
Zika virus Lymphocytes Monocytes Flow cytometry RT-qPCR |
| dc.subject.decs.pt_BR.fl_str_mv |
Reação em Cadeia da Polimerase em Tempo Real |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa sobre o Timo. Rio de Janeiro, RJ, Brasil Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto Nacional de Ciência e Tecnologia em Neuroimunomodulação. Rio de Janeiro, RJ, Brasil. |
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2019 |
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2019 |
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2020-04-30T18:26:39Z |
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2020-04-30T18:26:39Z |
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info:eu-repo/semantics/publishedVersion |
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MESSIAS, Carolina V. et al. Zika virus infects human blood mononuclear cells. BMC Infectious Diseases, v. 19, n. 986, p. 1-6, 2019. |
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https://www.arca.fiocruz.br/handle/icict/41050 |
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1471-2334 |
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10.1186/s12879-019-4622-y |
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MESSIAS, Carolina V. et al. Zika virus infects human blood mononuclear cells. BMC Infectious Diseases, v. 19, n. 986, p. 1-6, 2019. 1471-2334 10.1186/s12879-019-4622-y |
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BMC Infectious Disease |
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BMC Infectious Disease |
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