Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes

Detalhes bibliográficos
Autor(a) principal: Inoue, Alexandre Haruo
Data de Publicação: 2014
Outros Autores: Serpeloni, Mariana, Hiraiwa, Priscila Mazzocchi, Yamada-Ogatta, Sueli Fumie, Muniz, João Renato Carvalho, Motta, Maria Cristina Machado, Vidal, Newton Medeiros, Goldenberg, Samuel, Ávila, Andréa Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/16050
Resumo: Conselho Nacional de Desenvolvimento Cientıfico e Tecnologico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nıvel Superior (CAPES), Fundação Oswaldo Cruz (PAPES-FIOCRUZ Program) and Fundação Araucaria-PR.
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spelling Inoue, Alexandre HaruoSerpeloni, MarianaHiraiwa, Priscila MazzocchiYamada-Ogatta, Sueli FumieMuniz, João Renato CarvalhoMotta, Maria Cristina MachadoVidal, Newton MedeirosGoldenberg, SamuelÁvila, Andréa Rodrigues2016-10-03T16:43:16Z2016-10-03T16:43:16Z2014INOUE, Alexandre Haruo et al. Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes. PLoS ONE, v. 9, n. 10, p. 1-11, 2014.1932-6203https://www.arca.fiocruz.br/handle/icict/1605010.1371/journal.pone.0109521Conselho Nacional de Desenvolvimento Cientıfico e Tecnologico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nıvel Superior (CAPES), Fundação Oswaldo Cruz (PAPES-FIOCRUZ Program) and Fundação Araucaria-PR.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / Universidade Federal do Paraná. Laboratório de Biologia Celular e Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Universidade Estadual de Londrina. Departamento de Microbiologia. Londrina, PR, Brasil.Universidade de São Paulo. Instituto de Física de São Carlos. São Carlos, SP, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Departamento de Biologia Celular e Parasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil / National Institutes of Health. National Library of Medicine. National Center for Biotechnology Information. Bethesda, Maryland, USA.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Gene expression in trypanosomes is controlled mostly by post-transcriptional pathways. Little is known about the components of mRNA nucleocytoplasmic export routes in these parasites. Comparative genomics has shown that the mRNA transport pathway is the least conserved pathway among eukaryotes. Nonetheless, we identified a RNA helicase (Hel45) that is conserved across eukaryotes and similar to shuttling proteins involved in mRNA export. We used in silico analysis to predict the structure of Trypanosoma cruzi Hel45, including the N-terminal domain and the C-terminal domain, and our findings suggest that this RNA helicase can form complexes with mRNA. Hel45 was present in both nucleus and cytoplasm. Electron microscopy showed that Hel45 is clustered close to the cytoplasmic side of nuclear pore complexes, and is also present in the nucleus where it is associated with peripheral compact chromatin. Deletion of a predicted Nuclear Export Signal motif led to the accumulation of Hel45ΔNES in the nucleus, indicating that Hel45 shuttles between the nucleus and the cytoplasm. This transport was dependent on active transcription but did not depend on the exportin Crm1. Knockdown of Mex67 in T. brucei caused the nuclear accumulation of the T. brucei ortholog of Hel45. Indeed, Hel45 is present in mRNA ribonucleoprotein complexes that are not associated with polysomes. It is still necessary to confirm the precise function of Hel45. However, this RNA helicase is associated with mRNA metabolism and its nucleocytoplasmic shuttling is dependent on an mRNA export route involving Mex67 receptor.engIratxe PueblaIdentification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleRNA helicaseTrypanosomesNucleocytoplasmic shuttlingGene ExpressionExpressão GênicaTransporte Ativo do Núcleo CelularTrypanosomainfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83074https://www.arca.fiocruz.br/bitstream/icict/16050/1/license.txt0271fb68963c017aa933c7aff60379f4MD51ORIGINALIdentification of a novel nucleocytoplasmic shuttling RNA.pdfIdentification of a novel nucleocytoplasmic shuttling 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dc.title.pt_BR.fl_str_mv Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes
title Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes
spellingShingle Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes
Inoue, Alexandre Haruo
RNA helicase
Trypanosomes
Nucleocytoplasmic shuttling
Gene Expression
Expressão Gênica
Transporte Ativo do Núcleo Celular
Trypanosoma
title_short Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes
title_full Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes
title_fullStr Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes
title_full_unstemmed Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes
title_sort Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes
author Inoue, Alexandre Haruo
author_facet Inoue, Alexandre Haruo
Serpeloni, Mariana
Hiraiwa, Priscila Mazzocchi
Yamada-Ogatta, Sueli Fumie
Muniz, João Renato Carvalho
Motta, Maria Cristina Machado
Vidal, Newton Medeiros
Goldenberg, Samuel
Ávila, Andréa Rodrigues
author_role author
author2 Serpeloni, Mariana
Hiraiwa, Priscila Mazzocchi
Yamada-Ogatta, Sueli Fumie
Muniz, João Renato Carvalho
Motta, Maria Cristina Machado
Vidal, Newton Medeiros
Goldenberg, Samuel
Ávila, Andréa Rodrigues
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Inoue, Alexandre Haruo
Serpeloni, Mariana
Hiraiwa, Priscila Mazzocchi
Yamada-Ogatta, Sueli Fumie
Muniz, João Renato Carvalho
Motta, Maria Cristina Machado
Vidal, Newton Medeiros
Goldenberg, Samuel
Ávila, Andréa Rodrigues
dc.subject.en.pt_BR.fl_str_mv RNA helicase
Trypanosomes
Nucleocytoplasmic shuttling
topic RNA helicase
Trypanosomes
Nucleocytoplasmic shuttling
Gene Expression
Expressão Gênica
Transporte Ativo do Núcleo Celular
Trypanosoma
dc.subject.en.En.fl_str_mv Gene Expression
dc.subject.decs.pt_BR.fl_str_mv Expressão Gênica
Transporte Ativo do Núcleo Celular
Trypanosoma
description Conselho Nacional de Desenvolvimento Cientıfico e Tecnologico (CNPq), Coordenação de Aperfeiçoamento de Pessoal de Nıvel Superior (CAPES), Fundação Oswaldo Cruz (PAPES-FIOCRUZ Program) and Fundação Araucaria-PR.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2016-10-03T16:43:16Z
dc.date.available.fl_str_mv 2016-10-03T16:43:16Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv INOUE, Alexandre Haruo et al. Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes. PLoS ONE, v. 9, n. 10, p. 1-11, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/16050
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.pt_BR.fl_str_mv 10.1371/journal.pone.0109521
identifier_str_mv INOUE, Alexandre Haruo et al. Identification of a novel nucleocytoplasmic shuttling RNA helicase of Trypanosomes. PLoS ONE, v. 9, n. 10, p. 1-11, 2014.
1932-6203
10.1371/journal.pone.0109521
url https://www.arca.fiocruz.br/handle/icict/16050
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Iratxe Puebla
publisher.none.fl_str_mv Iratxe Puebla
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/16050/1/license.txt
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