Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.1080/15321810701454870 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/20125 |
Resumo: | Fundação Oswaldo Cruz (09224-7), Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (477609/2004-0,301920/2006-0 and 504227/2006-0) and National Institutes of Health, USA (AI034431 and TW00919). |
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Coutinho, Mariana LonerVasconcellos, Flavia AleixoFernandes, C. P. H.Seyffert, NúbiaSeixas, Fabiana KommlingKo, Albert IcksangDellagostin, Odir AntonioAleixo, José Antonio Guimarães2017-07-18T12:20:42Z2017-07-18T12:20:42Z2007COUTINHO, Mariana Loner et al. Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests. Journal of Immunoassay and Immunochemistry, v. 28, p. 279-288, 2007.1532-1819https://www.arca.fiocruz.br/handle/icict/2012510.1080/15321810701454870Fundação Oswaldo Cruz (09224-7), Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (477609/2004-0,301920/2006-0 and 504227/2006-0) and National Institutes of Health, USA (AI034431 and TW00919).Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RGS, Brazil.Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, BrazilFederal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, BrazilFederal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, BrazilFederal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil.Federal University of Pelotas. Center for Biotechnology. Porto Alegre, RS, Brazil.LipL32 is the major lipoprotein in the membrane of pathogenic leptospira. In this work, we report on the production of monoclonal antibodies (MAbs) against recombinant LipL32 (rLipL32) and on the evaluation of their potential for use as reagents in diagnostic tests for leptospirosis. The MAbs were all of the IgG(2b) isotype and reacted specifically with native LipL32 in pathogenic serovars only. MAbs reacted in the same region of the rLipL32 molecule and their affinity constant was between 5x10(7) M(-1) and 6x10(6) M(-1). These results suggest that although the MAbs cannot be used together, they are well suited for diagnostic tests of leptospirosis based on LipL32 detection.engTaylor & FrancisLeptospiraAntígenosAfinidade de anticorposMapeamento de epítoposELISAWestern BlotLeptospiraRecombinant antigens,Antibody affinityEpitope mappingELISAWestern blottingEvaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic testsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/20125/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCoutinho ML Evaluation of the anti....pdfCoutinho ML Evaluation of the 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| dc.title.pt_BR.fl_str_mv |
Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests |
| title |
Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests |
| spellingShingle |
Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests Coutinho, Mariana Loner Leptospira Antígenos Afinidade de anticorpos Mapeamento de epítopos ELISA Western Blot Leptospira Recombinant antigens, Antibody affinity Epitope mapping ELISA Western blotting |
| title_short |
Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests |
| title_full |
Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests |
| title_fullStr |
Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests |
| title_full_unstemmed |
Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests |
| title_sort |
Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests |
| author |
Coutinho, Mariana Loner |
| author_facet |
Coutinho, Mariana Loner Vasconcellos, Flavia Aleixo Fernandes, C. P. H. Seyffert, Núbia Seixas, Fabiana Kommling Ko, Albert Icksang Dellagostin, Odir Antonio Aleixo, José Antonio Guimarães |
| author_role |
author |
| author2 |
Vasconcellos, Flavia Aleixo Fernandes, C. P. H. Seyffert, Núbia Seixas, Fabiana Kommling Ko, Albert Icksang Dellagostin, Odir Antonio Aleixo, José Antonio Guimarães |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Coutinho, Mariana Loner Vasconcellos, Flavia Aleixo Fernandes, C. P. H. Seyffert, Núbia Seixas, Fabiana Kommling Ko, Albert Icksang Dellagostin, Odir Antonio Aleixo, José Antonio Guimarães |
| dc.subject.other.pt_BR.fl_str_mv |
Leptospira Antígenos Afinidade de anticorpos Mapeamento de epítopos ELISA Western Blot |
| topic |
Leptospira Antígenos Afinidade de anticorpos Mapeamento de epítopos ELISA Western Blot Leptospira Recombinant antigens, Antibody affinity Epitope mapping ELISA Western blotting |
| dc.subject.en.pt_BR.fl_str_mv |
Leptospira Recombinant antigens, Antibody affinity Epitope mapping ELISA Western blotting |
| description |
Fundação Oswaldo Cruz (09224-7), Conselho Nacional de Desenvolvimento Científico e Tecnológico - CNPq (477609/2004-0,301920/2006-0 and 504227/2006-0) and National Institutes of Health, USA (AI034431 and TW00919). |
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2007 |
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2007 |
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2017-07-18T12:20:42Z |
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2017-07-18T12:20:42Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
COUTINHO, Mariana Loner et al. Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests. Journal of Immunoassay and Immunochemistry, v. 28, p. 279-288, 2007. |
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https://www.arca.fiocruz.br/handle/icict/20125 |
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1532-1819 |
| dc.identifier.doi.none.fl_str_mv |
10.1080/15321810701454870 |
| identifier_str_mv |
COUTINHO, Mariana Loner et al. Evaluation of the anti-LipL32 monoclonal antibodies potential for use in leptospirosis immunodiagnostic tests. Journal of Immunoassay and Immunochemistry, v. 28, p. 279-288, 2007. 1532-1819 10.1080/15321810701454870 |
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Taylor & Francis |
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Taylor & Francis |
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