Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro

Detalhes bibliográficos
Autor(a) principal: Mazur, Karin Luize
Data de Publicação: 2018
Outros Autores: Feuser, Paulo Emílio, Valério, Alexsandra, Cordeiro, Arthur Poester, Oliveira, Camila Indiani de, Assolini, João Paulo, Pavanelli, Wander Rogério, Sayer, Claudia, Araújo, Pedro H. H
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31644
Resumo: CAPES−Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Tecnológico and CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico and Laboratório Central de Microscopia Eletrônica (LCME) of Federal University of Santa Catarina for the TEM analyses.
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spelling Mazur, Karin LuizeFeuser, Paulo EmílioValério, AlexsandraCordeiro, Arthur PoesterOliveira, Camila Indiani deAssolini, João PauloPavanelli, Wander RogérioSayer, ClaudiaAraújo, Pedro H. H2019-02-14T14:06:33Z2019-02-14T14:06:33Z2018MAZUR, K. L. et al. Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro. Colloids and Surfaces B: Biointerfaces, v. 176, p. 507–512, 2019.0927-7765https://www.arca.fiocruz.br/handle/icict/3164410.1016/j.colsurfb.2018.12.048CAPES−Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Tecnológico and CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico and Laboratório Central de Microscopia Eletrônica (LCME) of Federal University of Santa Catarina for the TEM analyses.Federal University of Santa Catarina. Department of Chemical Engineering and Food Engineering. Salvador, BA, Brazil.Federal University of Santa Catarina. Department of Chemical Engineering and Food Engineering. Salvador, BA, Brazil.Federal University of Santa Catarina. Department of Chemical Engineering and Food Engineering. Salvador, BA, Brazil.Federal University of Santa Catarina. Department of Chemical Engineering and Food Engineering. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.State University of Londrina. Center of Biological Sciences. Laboratory of Experimental Protozoology. Department of Pathological Sciences. Londrina, PR, Brazil.State University of Londrina. Center of Biological Sciences. Laboratory of Experimental Protozoology. Department of Pathological Sciences. Londrina, PR, Brazil.Federal University of Santa Catarina. Department of Chemical Engineering and Food Engineering. Salvador, BA, Brazil.Federal University of Santa Catarina. Department of Chemical Engineering and Food Engineering. Salvador, BA, Brazil.Leishmaniasis is considered a neglected tropical disease that represents a Public Health problem due to its high incidence. In the search of new alternatives for Leishmaniasis treatment diethyldithiocarbamate (DETC) has shown an excellent leishmanicidal activity and the incorporation into drug carrier systems, such as solid lipid nanoparticles (SLNs), is very promising. In the present work DETC loaded in beeswax nanoparticles containing copaiba oil were obtained by the double emulsion/melt technique. The nanoparticles were characterized and leishmanicidal activity against L. amazonensis promastigotes forms and cytotoxicity in murine macrophages were evaluated. SLNs presented size below 200 nm, spherical morphology, negative charge surface, high encapsulation efficiency, above 80%, and excellent stability. Moreover, Fourier transform infrared spectroscopy (FTIR) and differential scanning calorimetry (DSC) analyses were performed to evaluate the chemical structure and possible interactions between DETC and SLNs. SLNs provided a protection for DETC, decreasing its cytotoxic effects in macrophages, which led to an improvement in the selectivity against the parasites, which almost doubled from free DETC (11.4) to DETC incorporated in SLNs (18.2). These results demonstrated that SLNs had a direct effect on L. amazonensis promastigotes without affect the viability of macrophage cell, can be a promising alternative therapy for the cutaneous treatment of L. amazonensis.engElsevierNanopartículas lipídicas sólidasDietilditiocarbamatoDrogas HidrofílicasCera de AbelhaL. amazonensisSolid lipid nanoparticlesDiethyldithiocarbamateHydrophilic drugsBeeswaxL. amazonensisDiethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitroinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31644/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMazur L.M. 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dc.title.pt_BR.fl_str_mv Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro
title Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro
spellingShingle Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro
Mazur, Karin Luize
Nanopartículas lipídicas sólidas
Dietilditiocarbamato
Drogas Hidrofílicas
Cera de Abelha
L. amazonensis
Solid lipid nanoparticles
Diethyldithiocarbamate
Hydrophilic drugs
Beeswax
L. amazonensis
title_short Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro
title_full Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro
title_fullStr Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro
title_full_unstemmed Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro
title_sort Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro
author Mazur, Karin Luize
author_facet Mazur, Karin Luize
Feuser, Paulo Emílio
Valério, Alexsandra
Cordeiro, Arthur Poester
Oliveira, Camila Indiani de
Assolini, João Paulo
Pavanelli, Wander Rogério
Sayer, Claudia
Araújo, Pedro H. H
author_role author
author2 Feuser, Paulo Emílio
Valério, Alexsandra
Cordeiro, Arthur Poester
Oliveira, Camila Indiani de
Assolini, João Paulo
Pavanelli, Wander Rogério
Sayer, Claudia
Araújo, Pedro H. H
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Mazur, Karin Luize
Feuser, Paulo Emílio
Valério, Alexsandra
Cordeiro, Arthur Poester
Oliveira, Camila Indiani de
Assolini, João Paulo
Pavanelli, Wander Rogério
Sayer, Claudia
Araújo, Pedro H. H
dc.subject.other.pt_BR.fl_str_mv Nanopartículas lipídicas sólidas
Dietilditiocarbamato
Drogas Hidrofílicas
Cera de Abelha
L. amazonensis
topic Nanopartículas lipídicas sólidas
Dietilditiocarbamato
Drogas Hidrofílicas
Cera de Abelha
L. amazonensis
Solid lipid nanoparticles
Diethyldithiocarbamate
Hydrophilic drugs
Beeswax
L. amazonensis
dc.subject.en.pt_BR.fl_str_mv Solid lipid nanoparticles
Diethyldithiocarbamate
Hydrophilic drugs
Beeswax
L. amazonensis
description CAPES−Coordenação de Aperfeiçoamento de Pessoal de Nível Superior Tecnológico and CNPq - Conselho Nacional de Desenvolvimento Científico e Tecnológico and Laboratório Central de Microscopia Eletrônica (LCME) of Federal University of Santa Catarina for the TEM analyses.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-02-14T14:06:33Z
dc.date.available.fl_str_mv 2019-02-14T14:06:33Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv MAZUR, K. L. et al. Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro. Colloids and Surfaces B: Biointerfaces, v. 176, p. 507–512, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31644
dc.identifier.issn.pt_BR.fl_str_mv 0927-7765
dc.identifier.doi.none.fl_str_mv 10.1016/j.colsurfb.2018.12.048
identifier_str_mv MAZUR, K. L. et al. Diethyldithiocarbamate loaded in beeswax-copaiba oil nanoparticles obtained by solventless double emulsion technique promote promastigote death in vitro. Colloids and Surfaces B: Biointerfaces, v. 176, p. 507–512, 2019.
0927-7765
10.1016/j.colsurfb.2018.12.048
url https://www.arca.fiocruz.br/handle/icict/31644
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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