Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/48425 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil. |
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Calegar, Deiviane AparecidaMonteiro, Kerla Joeline LimaBacelar, Polyanna Araújo AlvesEvangelista, Brenda Bulsara CostaAlmeida, Mayron MoraisSantos, Jéssica Pereira dosBoia, Márcio NevesNunes, Beatriz CoronatoJaeger, Lauren HubertCosta, Filipe Anibal Carvalho2021-07-30T14:11:07Z2021-07-30T14:11:07Z2021CALEGAR, Deiviane Aparecida et al. Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes. Parasites & Vectors, v. 14, n. 160, 13 p, 2021.1756-3305https://www.arca.fiocruz.br/handle/icict/4842510.1186/s13071-021-04672-yengBMCGênero EntamoebaEpidemiologia molecularDNA ribossômico de subunidade pequenaIntra e interespecíficoEntamoeba genusMolecular epidemiologySmall subunit ribosomal DNAIntra- and interspecifcEpidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz Piauí. Centro/Norte Escritório Técnico Regional. Terezina, PI, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz Piauí. Centro/Norte Escritório Técnico Regional. Terezina, PI, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz Piauí. Centro/Norte Escritório Técnico Regional. Terezina, PI, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz Piauí. Centro/Norte Escritório Técnico Regional. Terezina, PI, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Faculdade de Medicina de Petrópolis (FMP)/ Faculdade Arthur Sá Earp Neto (FASE). Petrópolis, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Universidade Federal de Juiz de Fora. Faculdade de Farmácia. Campus Universitário Bairro São Pedro. Juiz de Fora, MG, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Background: Entamoeba species harbored by humans have diferent degrees of pathogenicity. The present study explores the intra- and interspecifc diversity, phylogenetic relationships, prevalence and distribution of tetra- and octonucleated cyst-producing Entamoeba in diferent Brazilian regions. Methods: Cross-sectional studies were performed to collect fecal samples (n=1728) and sociodemographic data in communities located in four Brazilian biomes: Atlantic Forest, Caatinga, Cerrado, and Amazon. Fecal samples were subjected to molecular analysis by partial small subunit ribosomal DNA sequencing (SSU rDNA) and phylogenetic analysis. Results: Light microscopy analysis revealed that tetranucleated cysts were found in all the studied biomes. The highest positivity rates were observed in the age group 6–10 years (23.21%). For octonucleated cysts, positivity rates ranged from 1 to 55.1%. Sixty SSU rDNA Entamoeba sequences were obtained, and four diferent species were identifed: the octonucleated E. coli, and the tetranucleated E. histolytica, E. dispar, and E. hartmanni. Novel haplotypes (n=32) were characterized; however, new ribosomal lineages were not identifed. The Entamoeba coli ST1 subtype predominated in Atlantic Forest and Caatinga, and the ST2 subtype was predominant in the Amazon biome. E. histol ytica was detected only in the Amazon biome. In phylogenetic trees, sequences were grouped in two groups, the frst containing uni- and tetranucleated and the second containing uni- and octonucleated cyst-producing Entamoeba species. Molecular diversity indexes revealed a high interspecifc diversity for tetra- and octonucleated Entamoeba spp. (H±SD=0.9625±0.0126). The intraspecifc diversity varied according to species or subtype: E. dispar and E. histo lytica showed lower diversity than E. coli subtypes ST1 and ST2 and E. hartmanni. Conclusions: Tetra- and octonucleated cyst-producing Entamoeba are endemic in the studied communities; E. histolytica was found in a low proportion and only in the Amazon biome. With regard to E. coli, subtype ST2 was predominant in the Amazon biome. The molecular epidemiology of Entamoeba spp. is a feld to be further explored and provides information with important implications for public health.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48425/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALDeivianeAparecida_etal_IOC_2021.pdfDeivianeAparecida_etal_IOC_2021.pdfapplication/pdf3180442https://www.arca.fiocruz.br/bitstream/icict/48425/2/DeivianeAparecida_etal_IOC_2021.pdf463e854afa0a23df23a78f77b9237ba8MD52TEXTDeivianeAparecida_etal_IOC_2021.pdf.txtDeivianeAparecida_etal_IOC_2021.pdf.txtExtracted texttext/plain51561https://www.arca.fiocruz.br/bitstream/icict/48425/3/DeivianeAparecida_etal_IOC_2021.pdf.txt60c4bcb51f672f86f4b61af996467cedMD53icict/484252021-07-31 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| dc.title.pt_BR.fl_str_mv |
Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes |
| title |
Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes |
| spellingShingle |
Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes Calegar, Deiviane Aparecida Gênero Entamoeba Epidemiologia molecular DNA ribossômico de subunidade pequena Intra e interespecífico Entamoeba genus Molecular epidemiology Small subunit ribosomal DNA Intra- and interspecifc |
| title_short |
Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes |
| title_full |
Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes |
| title_fullStr |
Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes |
| title_full_unstemmed |
Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes |
| title_sort |
Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes |
| author |
Calegar, Deiviane Aparecida |
| author_facet |
Calegar, Deiviane Aparecida Monteiro, Kerla Joeline Lima Bacelar, Polyanna Araújo Alves Evangelista, Brenda Bulsara Costa Almeida, Mayron Morais Santos, Jéssica Pereira dos Boia, Márcio Neves Nunes, Beatriz Coronato Jaeger, Lauren Hubert Costa, Filipe Anibal Carvalho |
| author_role |
author |
| author2 |
Monteiro, Kerla Joeline Lima Bacelar, Polyanna Araújo Alves Evangelista, Brenda Bulsara Costa Almeida, Mayron Morais Santos, Jéssica Pereira dos Boia, Márcio Neves Nunes, Beatriz Coronato Jaeger, Lauren Hubert Costa, Filipe Anibal Carvalho |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Calegar, Deiviane Aparecida Monteiro, Kerla Joeline Lima Bacelar, Polyanna Araújo Alves Evangelista, Brenda Bulsara Costa Almeida, Mayron Morais Santos, Jéssica Pereira dos Boia, Márcio Neves Nunes, Beatriz Coronato Jaeger, Lauren Hubert Costa, Filipe Anibal Carvalho |
| dc.subject.other.pt_BR.fl_str_mv |
Gênero Entamoeba Epidemiologia molecular DNA ribossômico de subunidade pequena Intra e interespecífico |
| topic |
Gênero Entamoeba Epidemiologia molecular DNA ribossômico de subunidade pequena Intra e interespecífico Entamoeba genus Molecular epidemiology Small subunit ribosomal DNA Intra- and interspecifc |
| dc.subject.en.pt_BR.fl_str_mv |
Entamoeba genus Molecular epidemiology Small subunit ribosomal DNA Intra- and interspecifc |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil. |
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2021 |
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2021-07-30T14:11:07Z |
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2021-07-30T14:11:07Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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CALEGAR, Deiviane Aparecida et al. Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes. Parasites & Vectors, v. 14, n. 160, 13 p, 2021. |
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https://www.arca.fiocruz.br/handle/icict/48425 |
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1756-3305 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/s13071-021-04672-y |
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CALEGAR, Deiviane Aparecida et al. Epidemiology, species composition and genetic diversity of tetra and octonucleated Entamoeba spp. in diferent Brazilian biomes. Parasites & Vectors, v. 14, n. 160, 13 p, 2021. 1756-3305 10.1186/s13071-021-04672-y |
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