Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily better
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.3390/jof6040311 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/51068 |
Resumo: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (CNPq 564507/2010-5 a 2018) e pela Fundação de Apoio à Pesquisa do Distrito Federal (FAP-DF)/CNPq PRONEX (Bolsas: 0193.000. 571/2009, 0193.000.496/2009 e 0193.001.200/2016). |
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Silva, Calliandra Maria de SouzaHurtado, Fabián AndrésTavares, Aldo Henrique Fonseca PachecoOliveira Júnior, Getúlio Pereira deRaiol, TaináNishibe, ChristianeAgustinho, Daniel PaivaAlmeida, Nalvo FrancoWalter, Maria Emília Machado TellesNicola, André MoraesBocca, Anamélia LorenzettiAlbuquerque, Patrícia2022-02-08T13:10:51Z2022-02-08T13:10:51Z2020DE-SOUZA-SILVA, Calliandra M. et al. Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily better. Journal of Fungi, [s. l.], v. 6, n. 4, 311, 2020.2309-608Xhttps://www.arca.fiocruz.br/handle/icict/5106810.3390/jof6040311Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (CNPq 564507/2010-5 a 2018) e pela Fundação de Apoio à Pesquisa do Distrito Federal (FAP-DF)/CNPq PRONEX (Bolsas: 0193.000. 571/2009, 0193.000.496/2009 e 0193.001.200/2016).University of Brasília. Institute of Biological Sciences. Department of Cell Biology. Laboratory of Molecular Biology of Pathogenic Fungi. Brasília, DF, Brazil.University of Brasília. Institute of Biological Sciences. Department of Cell Biology. Laboratory of Molecular Biology of Pathogenic Fungi. Brasília, DF, Brazil / University of Brasília. Medical School. Molecular Pathology Post-Graduation Program. Brasília, DF, Brazil.University of Brasília. Faculty of Ceilândia. Brasília, DF, Brazil.Harvard Medical School. Beth Israel Deaconess Medical Center. Department of Medicine. Division of Allergy and Inflammation. Boston, MA, United States.Fundação Oswaldo Cruz. Fiocruz Brasília. Brasília, DF, Brasil.Federal University of Mato Grosso do Sul. Faculty of Computing. Campo Grande, MS, Brazil.Washington University School of Medicine. Department of Molecular Microbiology. St. Louis, MO, United States.Federal University of Mato Grosso do Sul. Faculty of Computing. Campo Grande, MS, Brazil.University of Brasília. Department of Computer Science. Brasília, DF, Brazil.University of Brasília. Faculty of Medicine. Brasília, DF, Brazil.University of Brasília. Institute of Biological Sciences. Department of Cell Biology. Laboratory of Applied Immunology. Brasília, DF, Brazil.University of Brasília. Institute of Biological Sciences. Department of Cell Biology. Laboratory of Molecular Biology of Pathogenic Fungi. Brasília, DF, Brazil / University of Brasília. Faculty of Ceilândia. Brasília, DF, Brazil.University of Brasília. Institute of Biological Sciences. Department of Cell Biology. Laboratory of Molecular Biology of Pathogenic Fungi. Brasília, DF, Brazil / University of Brasília. Medical School. Molecular Pathology Post-Graduation Program. Brasília, DF, Brazil.Most people infected with the fungus Paracoccidioides spp. do not get sick, but approximately 5% develop paracoccidioidomycosis. Understanding how host immunity determinants influence disease development could lead to novel preventative or therapeutic strategies; hence, we used two mouse strains that are resistant (A/J) or susceptible (B10.A) to P. brasiliensis to study how dendritic cells (DCs) respond to the infection. RNA sequencing analysis showed that the susceptible strain DCs remodeled their transcriptomes much more intensely than those from the resistant strain, agreeing with a previous model of more intense innate immunity response in the susceptible strain. Contrastingly, these cells also repress genes/processes involved in antigen processing and presentation, such as lysosomal activity and autophagy. After the interaction with P. brasiliensis, both DCs and macrophages from the susceptible mouse reduced the autophagy marker LC3-II recruitment to the fungal phagosome compared to the resistant strain cells, confirming this pathway’s repression. These results suggest that impairment in antigen processing and presentation processes might be partially responsible for the inefficient activation of the adaptive immune response in this model.engMDPIParacoccidioidesImmunity, InnateDendritic CellsParacoccidioides brasiliensisFungal innate immunityDendritic cellsResistanceSusceptibilityA/J and B10, A mouse strainsAutophagyParacoccidioidesImunidade InataFungosCélulas DendríticasAutofagiaTranscriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily betterinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily better |
| title |
Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily better |
| spellingShingle |
Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily better Silva, Calliandra Maria de Souza Paracoccidioides Immunity, Innate Dendritic Cells Paracoccidioides brasiliensis Fungal innate immunity Dendritic cells Resistance Susceptibility A/J and B10, A mouse strains Autophagy Paracoccidioides Imunidade Inata Fungos Células Dendríticas Autofagia |
| title_short |
Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily better |
| title_full |
Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily better |
| title_fullStr |
Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily better |
| title_full_unstemmed |
Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily better |
| title_sort |
Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily better |
| author |
Silva, Calliandra Maria de Souza |
| author_facet |
Silva, Calliandra Maria de Souza Hurtado, Fabián Andrés Tavares, Aldo Henrique Fonseca Pacheco Oliveira Júnior, Getúlio Pereira de Raiol, Tainá Nishibe, Christiane Agustinho, Daniel Paiva Almeida, Nalvo Franco Walter, Maria Emília Machado Telles Nicola, André Moraes Bocca, Anamélia Lorenzetti Albuquerque, Patrícia |
| author_role |
author |
| author2 |
Hurtado, Fabián Andrés Tavares, Aldo Henrique Fonseca Pacheco Oliveira Júnior, Getúlio Pereira de Raiol, Tainá Nishibe, Christiane Agustinho, Daniel Paiva Almeida, Nalvo Franco Walter, Maria Emília Machado Telles Nicola, André Moraes Bocca, Anamélia Lorenzetti Albuquerque, Patrícia |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Calliandra Maria de Souza Hurtado, Fabián Andrés Tavares, Aldo Henrique Fonseca Pacheco Oliveira Júnior, Getúlio Pereira de Raiol, Tainá Nishibe, Christiane Agustinho, Daniel Paiva Almeida, Nalvo Franco Walter, Maria Emília Machado Telles Nicola, André Moraes Bocca, Anamélia Lorenzetti Albuquerque, Patrícia |
| dc.subject.mesh.pt_BR.fl_str_mv |
Paracoccidioides Immunity, Innate Dendritic Cells |
| topic |
Paracoccidioides Immunity, Innate Dendritic Cells Paracoccidioides brasiliensis Fungal innate immunity Dendritic cells Resistance Susceptibility A/J and B10, A mouse strains Autophagy Paracoccidioides Imunidade Inata Fungos Células Dendríticas Autofagia |
| dc.subject.en.pt_BR.fl_str_mv |
Paracoccidioides brasiliensis Fungal innate immunity Dendritic cells Resistance Susceptibility A/J and B10, A mouse strains Autophagy |
| dc.subject.decs.pt_BR.fl_str_mv |
Paracoccidioides Imunidade Inata Fungos Células Dendríticas Autofagia |
| description |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) (CNPq 564507/2010-5 a 2018) e pela Fundação de Apoio à Pesquisa do Distrito Federal (FAP-DF)/CNPq PRONEX (Bolsas: 0193.000. 571/2009, 0193.000.496/2009 e 0193.001.200/2016). |
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2020 |
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2020 |
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2022-02-08T13:10:51Z |
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2022-02-08T13:10:51Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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DE-SOUZA-SILVA, Calliandra M. et al. Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily better. Journal of Fungi, [s. l.], v. 6, n. 4, 311, 2020. |
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https://www.arca.fiocruz.br/handle/icict/51068 |
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2309-608X |
| dc.identifier.doi.none.fl_str_mv |
10.3390/jof6040311 |
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DE-SOUZA-SILVA, Calliandra M. et al. Transcriptional remodeling patterns in murine dendritic cells infected with Paracoccidioides brasiliensis: more is not necessarily better. Journal of Fungi, [s. l.], v. 6, n. 4, 311, 2020. 2309-608X 10.3390/jof6040311 |
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eng |
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MDPI |
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MDPI |
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