Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/32003 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. |
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Cataneo, Allan Henrique DepieriKuczera, DiogoMosimann, Ana Luiza PamplonaSilva, Emanuele GuimarãesFerreira, Álvaro Gil AraújoMarques, João TrindadeWowk, Pryscilla FaniniSantos, Claudia Nunes Duarte dosBordignon, Juliano2019-03-08T15:16:40Z2019-03-08T15:16:40Z2019CATANEO, Allan Henrique Depieri et al. Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-8, 2019.1678-8060https://www.arca.fiocruz.br/handle/icict/3200310.1590/0074-02760180432porFundação Oswaldo Cruz. Instituto Oswaldo CruzDensovirusArbovirusesContaminationZika VirusArbovirusContaminaciónVirus ZikaArbovirusDensovirusContaminaçãoDetection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil.The Zika virus (ZIKV) epidemics that affected South America in 2016 raised several research questions and prompted an increase in studies in the field. The transient and low viraemia observed in the course of ZIKV infection is a challenge for viral isolation from patient serum, which leads to many laboratories around the world sharing viral strains for their studies. C6/36 cells derived from Aedes albopictus larvae are commonly used for arbovirus isolation from clinical samples and for the preparation of viral stocks. Here, we report the contamination of two widely used ZIKV strains by Brevidensovirus, here designated as mosquito densovirus (MDV). With methodology, Molecular and immunological techniques were used to analyse the MDV contamination of ZIKV stocks. Also, virus passages in mammalian cell line and infecting susceptible mice were used to MDV clearance from ZIKV stocks. MDV contamination was confirmed by molecular and immunological techniques and likely originated from C6/36 cultures commonly used to grow viral stocks. We applied two protocols that successfully eliminated MDV contamination from ZIKV stocks, and these protocols can be widely applied in the field. As MDV does not infect vertebrate cells, we performed serial passages of contaminated stocks using a mammalian cell line and infecting susceptible mice prior to re-isolating ZIKV from the animals’ blood serum. MDV elimination was confirmed with immunostaining, polymerase chain reaction (PCR), and analysis of the mosquitoes that were allowed to feed on the infected mice. How main conclusion since the putative impact of viral contaminants in ZIKV strains generally used for research purposes is unknown, researchers working in the field must be aware of potential contaminants and test viral stocks to certify sample purity.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/32003/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALDetection_Clearance_Mosq.pdfDetection_Clearance_Mosq.pdfapplication/pdf4297698https://www.arca.fiocruz.br/bitstream/icict/32003/2/Detection_Clearance_Mosq.pdf760b2f4c167d51658b70be8a90329774MD52TEXTDetection_Clearance_Mosq.pdf.txtDetection_Clearance_Mosq.pdf.txtExtracted texttext/plain34891https://www.arca.fiocruz.br/bitstream/icict/32003/3/Detection_Clearance_Mosq.pdf.txt74b197a301dfbb930f295e68c95ccbeaMD53icict/320032019-11-01 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| dc.title.pt_BR.fl_str_mv |
Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus |
| title |
Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus |
| spellingShingle |
Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus Cataneo, Allan Henrique Depieri Densovirus Arboviruses Contamination Zika Virus Arbovirus Contaminación Virus Zika Arbovirus Densovirus Contaminação |
| title_short |
Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus |
| title_full |
Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus |
| title_fullStr |
Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus |
| title_full_unstemmed |
Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus |
| title_sort |
Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus |
| author |
Cataneo, Allan Henrique Depieri |
| author_facet |
Cataneo, Allan Henrique Depieri Kuczera, Diogo Mosimann, Ana Luiza Pamplona Silva, Emanuele Guimarães Ferreira, Álvaro Gil Araújo Marques, João Trindade Wowk, Pryscilla Fanini Santos, Claudia Nunes Duarte dos Bordignon, Juliano |
| author_role |
author |
| author2 |
Kuczera, Diogo Mosimann, Ana Luiza Pamplona Silva, Emanuele Guimarães Ferreira, Álvaro Gil Araújo Marques, João Trindade Wowk, Pryscilla Fanini Santos, Claudia Nunes Duarte dos Bordignon, Juliano |
| author2_role |
author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Cataneo, Allan Henrique Depieri Kuczera, Diogo Mosimann, Ana Luiza Pamplona Silva, Emanuele Guimarães Ferreira, Álvaro Gil Araújo Marques, João Trindade Wowk, Pryscilla Fanini Santos, Claudia Nunes Duarte dos Bordignon, Juliano |
| dc.subject.other.pt_BR.fl_str_mv |
Densovirus |
| topic |
Densovirus Arboviruses Contamination Zika Virus Arbovirus Contaminación Virus Zika Arbovirus Densovirus Contaminação |
| dc.subject.en.pt_BR.fl_str_mv |
Arboviruses Contamination Zika Virus |
| dc.subject.es.pt_BR.fl_str_mv |
Arbovirus Contaminación Virus Zika |
| dc.subject.decs.pt_BR.fl_str_mv |
Arbovirus Densovirus Contaminação |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Laboratório de Virologia Molecular. Curitiba, PR, Brasil. |
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2019 |
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2019-03-08T15:16:40Z |
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2019-03-08T15:16:40Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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CATANEO, Allan Henrique Depieri et al. Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-8, 2019. |
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https://www.arca.fiocruz.br/handle/icict/32003 |
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1678-8060 |
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10.1590/0074-02760180432 |
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CATANEO, Allan Henrique Depieri et al. Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-8, 2019. 1678-8060 10.1590/0074-02760180432 |
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