Reduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infection
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| DOI: | 10.3389/fmicb.2019.00145 |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/32074 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. |
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Leite, Thaysse FerreiraDelatorre, EdsonCôrtes, Fernanda HeloiseFerreira, Ana Cristina GarciaCardoso, Sandra WagnerGrinsztejn, BeatrizAndrade, Michelle Morata deVeloso, Valdiléa G.Morgado, Mariza GonçalvesGuimarães, Monick Lindenmeyer2019-03-14T12:52:07Z2019-03-14T12:52:07Z2019LEITE, Thaysse Ferreira et al. Reduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infection. Frontiers in Microbiology, v. 10, p. 1-12, Feb. 2019.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/3207410.3389/fmicb.2019.001451664-302XengFrontiers MediaReduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST e AIDS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST e AIDS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST e AIDS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST e AIDS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Pesquisa Clínica em DST e AIDS. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil.The aim of early combined antiretroviral therapy (cART) of HIV is to limit the seeding of the viral reservoir during the initial phase of infection and, consequently, decrease intrahost viral diversity. Here, we assessed the effect of early cART on size and complexity of the proviral reservoir. Peripheral blood mononuclear cell (PBMC) and plasma samples were obtained from ten HIV-infected Brazilian individuals diagnosed at the acute phase of infection, before (PREART) and 12 months (M12ART) after suppressive cART. HIV proviral reservoir size was determined by quantitative real-time PCR; intrahost viral diversity of the env C2-V3 region was assessed by single genome amplification or next-generation sequencing in PBMC and plasma, respectively. Mean nucleotide diversity (π) and normalized Shannon entropy (HSN) were used to infer the complexity of the viral population. Compared to PREART, M12ART saw an immunological recovery with a gain of ∼200 CD4+ T cells (P = 0.008) and a normalization of the CD4/CD8 ratio [1.0 (IQR: 0.88–1.18), P = 0.016], as well as a significant decrease in HIV-1 RNA (∼4 log, P = 0.004) and DNA (∼1 log, P = 0.002) levels. The median time to achieve viral suppression was 3 months (IQR: 2.8–5.8 months). The high intermixing between sequences from both visits suggests that the HIV-1 DNA reservoir remained remarkably stable under cART. After 1 year of cART, there was a minor reduction in proviral π (PreART = 0.20 vs. M12ART = 0.10; P = 0.156) but a significant decrease in HSN (PreART = 0.41 vs. M12ART = 0.25; P = 0.019). We found no correlation between π or HSN at PreART and the rate of HIV DNA decay, T CD4+ counts, or CD4/CD8 ratio at M12ART. Based on a small cohort of Brazilian infected individuals under early cART and analyses of the env region, 1 year of follow-up suggested a reservoir size reduction, allowed a significant decrease of HIV-1 complexity, and achieved immunological restoration regardless of the initial HIV-1 plasma viral load, CD4+ T cell counts, or HIV-1 subtype. However, further studies in the Brazilian setting aiming a longer follow-up and larger cohort are required in this field.HIV-1ReservoirDiversityEarly cARTAcute infectioninfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/32074/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Leite_Thaysse_etal_INI_2019.pdfve_Leite_Thaysse_etal_INI_2019.pdfapplication/pdf2036612https://www.arca.fiocruz.br/bitstream/icict/32074/2/ve_Leite_Thaysse_etal_INI_2019.pdfa8026d4ef981e4f09079d6bcb9391788MD52TEXTve_Leite_Thaysse_etal_INI_2019.pdf.txtve_Leite_Thaysse_etal_INI_2019.pdf.txtExtracted texttext/plain55301https://www.arca.fiocruz.br/bitstream/icict/32074/3/ve_Leite_Thaysse_etal_INI_2019.pdf.txt8708d3168de05e710f72f7d00b33d1ebMD53icict/320742023-03-30 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| dc.title.pt_BR.fl_str_mv |
Reduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infection |
| title |
Reduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infection |
| spellingShingle |
Reduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infection Leite, Thaysse Ferreira HIV-1 Reservoir Diversity Early cART Acute infection |
| title_short |
Reduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infection |
| title_full |
Reduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infection |
| title_fullStr |
Reduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infection |
| title_full_unstemmed |
Reduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infection |
| title_sort |
Reduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infection |
| author |
Leite, Thaysse Ferreira |
| author_facet |
Leite, Thaysse Ferreira Delatorre, Edson Côrtes, Fernanda Heloise Ferreira, Ana Cristina Garcia Cardoso, Sandra Wagner Grinsztejn, Beatriz Andrade, Michelle Morata de Veloso, Valdiléa G. Morgado, Mariza Gonçalves Guimarães, Monick Lindenmeyer |
| author_role |
author |
| author2 |
Delatorre, Edson Côrtes, Fernanda Heloise Ferreira, Ana Cristina Garcia Cardoso, Sandra Wagner Grinsztejn, Beatriz Andrade, Michelle Morata de Veloso, Valdiléa G. Morgado, Mariza Gonçalves Guimarães, Monick Lindenmeyer |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Leite, Thaysse Ferreira Delatorre, Edson Côrtes, Fernanda Heloise Ferreira, Ana Cristina Garcia Cardoso, Sandra Wagner Grinsztejn, Beatriz Andrade, Michelle Morata de Veloso, Valdiléa G. Morgado, Mariza Gonçalves Guimarães, Monick Lindenmeyer |
| dc.subject.en.pt_BR.fl_str_mv |
HIV-1 Reservoir Diversity Early cART Acute infection |
| topic |
HIV-1 Reservoir Diversity Early cART Acute infection |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de AIDS e Imunologia Molecular. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2019 |
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2019-03-14T12:52:07Z |
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2019-03-14T12:52:07Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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LEITE, Thaysse Ferreira et al. Reduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infection. Frontiers in Microbiology, v. 10, p. 1-12, Feb. 2019. |
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https://www.arca.fiocruz.br/handle/icict/32074 |
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1664-302X |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fmicb.2019.00145 |
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1664-302X |
| identifier_str_mv |
LEITE, Thaysse Ferreira et al. Reduction of HIV-1 reservoir size and diversity after 1 year of cART among Brazilian individuals starting treatment during early stages of acute infection. Frontiers in Microbiology, v. 10, p. 1-12, Feb. 2019. 1664-302X 10.3389/fmicb.2019.00145 |
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Frontiers Media |
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Frontiers Media |
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