Antibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazon
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/40286 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. |
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Matos, Ada da SilvaSilva, Rodrigo Nunes Rodrigues daSoares, Isabela FerreiraBaptista, Barbara de OliveiraSouza, Rodrigo Medeiros deChaves, Lana BitencourtTotino, Paulo Renato RivasSánchez-Arcila, Juan CamiloDaniel-Ribeiro, Cláudio TadeuLópez-Camacho, CésarReyes-Sandoval, ArturoPratt-Riccio, Lilian RoseLima Junior, Josué da Costa2020-03-12T11:14:32Z2020-03-12T11:14:32Z2019MATOS, Ada da Silva et al. Antibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazon. Frontiers in Immunology, v. 10, p. 1-7, Sept. 2019.1664-3224https://www.arca.fiocruz.br/handle/icict/4028610.3389/fimmu.2019.02230engFrontiers MediaMaláriaPlasmodiium vivaxMapeamento de epítopoResposta imune humoralEpitope mappingHumoral immune responseMalariaPlasmodiium vivaxPvTRAPAntibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazoninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Tecnologia em Imunobiológicos. Laboratório de Tecnologia de Anticorpos Monoclonais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Universidade Federal do Acre. Centro de Ciências da Saúde e Esporte. Cruzeiro do Sul, AC, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.University of Oxford. The Jenner Institute. Nuffield Department of Medicine. Oxford, United Kingdom.University of Oxford. The Jenner Institute. Nuffield Department of Medicine. Oxford, United Kingdom.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil.Thrombospondin-related adhesive protein (TRAP) is essential for sporozoite motility and the invasion of mosquitoes' salivary gland and vertebrate's hepatocyte and is, thus, considered a promising pre-erythrocytic vaccine candidate. Despite the existence of a few reports on naturally acquired immune response against Plasmodium vivax TRAP (PvTRAP), it has never been explored so far in the Amazon region, so results are conflicting. Here, we characterized the (IgG and IgG subclass) antibody reactivity against recombinant PvTRAP in a cross-sectional study of 299 individuals exposed to malaria infection in three municipalities (Cruzeiro do Sul, Mâncio Lima and Guajará) from the Acre state of the Brazilian Amazon. In addition, the full PvTRAP sequence was screened for B-cell epitopes using in silico and in vitro approaches. Firstly, we confirmed that PvTRAP is naturally immunogenic in the cohort population since 49% of the individuals were IgG-responders to it. The observed immune responses were mainly driven by cytophilic IgG1 over all other sublcasses and the IgG levels that was corelated with age and time of residence in the studied area (p < 0.05). Interestingly, only the levels of specific anti-TRAP IgG3 seemed to be associated with protection, as IgG3 responders presented a significantly higher time elapse since the last malaria episode than those recorded for IgG3 non-responders. Regarding the B-cell epitope mapping, among the 148 responders to PvTRAP, four predicted epitopes were confirmed by recognition of antibodies (PvTRAPR197-H227; PvTRAPE237-T258; PvTRAPP344-G374; and PvTRAPE439-K454). Nevertheless, the frequency of responders against these peptides were low and did not show a clear correlation with the antibody response against the corresponding antigen. Moreover, none of the linear confirmed epitopes were located in the binding regions of PvTRAP in respect to the host cell ligand. Collectively, our data confirm the PvTRAP immunogenicity among Amazon inhabitants, while suggesting that the main important B-cell epitopes are not linear.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40286/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALLilianPratt_Riccio_etal_IOC_2019.pdfLilianPratt_Riccio_etal_IOC_2019.pdfapplication/pdf1011549https://www.arca.fiocruz.br/bitstream/icict/40286/2/LilianPratt_Riccio_etal_IOC_2019.pdf370bdb0295558b7c42cc342c35b216bdMD52TEXTLilianPratt_Riccio_etal_IOC_2019.pdf.txtLilianPratt_Riccio_etal_IOC_2019.pdf.txtExtracted texttext/plain60263https://www.arca.fiocruz.br/bitstream/icict/40286/3/LilianPratt_Riccio_etal_IOC_2019.pdf.txtbd0b16717860f808dec8fe5054fae23dMD53icict/402862022-06-24 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| dc.title.pt_BR.fl_str_mv |
Antibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazon |
| title |
Antibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazon |
| spellingShingle |
Antibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazon Matos, Ada da Silva Malária Plasmodiium vivax Mapeamento de epítopo Resposta imune humoral Epitope mapping Humoral immune response Malaria Plasmodiium vivax PvTRAP |
| title_short |
Antibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazon |
| title_full |
Antibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazon |
| title_fullStr |
Antibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazon |
| title_full_unstemmed |
Antibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazon |
| title_sort |
Antibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazon |
| author |
Matos, Ada da Silva |
| author_facet |
Matos, Ada da Silva Silva, Rodrigo Nunes Rodrigues da Soares, Isabela Ferreira Baptista, Barbara de Oliveira Souza, Rodrigo Medeiros de Chaves, Lana Bitencourt Totino, Paulo Renato Rivas Sánchez-Arcila, Juan Camilo Daniel-Ribeiro, Cláudio Tadeu López-Camacho, César Reyes-Sandoval, Arturo Pratt-Riccio, Lilian Rose Lima Junior, Josué da Costa |
| author_role |
author |
| author2 |
Silva, Rodrigo Nunes Rodrigues da Soares, Isabela Ferreira Baptista, Barbara de Oliveira Souza, Rodrigo Medeiros de Chaves, Lana Bitencourt Totino, Paulo Renato Rivas Sánchez-Arcila, Juan Camilo Daniel-Ribeiro, Cláudio Tadeu López-Camacho, César Reyes-Sandoval, Arturo Pratt-Riccio, Lilian Rose Lima Junior, Josué da Costa |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Matos, Ada da Silva Silva, Rodrigo Nunes Rodrigues da Soares, Isabela Ferreira Baptista, Barbara de Oliveira Souza, Rodrigo Medeiros de Chaves, Lana Bitencourt Totino, Paulo Renato Rivas Sánchez-Arcila, Juan Camilo Daniel-Ribeiro, Cláudio Tadeu López-Camacho, César Reyes-Sandoval, Arturo Pratt-Riccio, Lilian Rose Lima Junior, Josué da Costa |
| dc.subject.other.pt_BR.fl_str_mv |
Malária Plasmodiium vivax Mapeamento de epítopo Resposta imune humoral |
| topic |
Malária Plasmodiium vivax Mapeamento de epítopo Resposta imune humoral Epitope mapping Humoral immune response Malaria Plasmodiium vivax PvTRAP |
| dc.subject.en.pt_BR.fl_str_mv |
Epitope mapping Humoral immune response Malaria Plasmodiium vivax PvTRAP |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ, Brasil. |
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2019 |
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2019 |
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2020-03-12T11:14:32Z |
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2020-03-12T11:14:32Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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MATOS, Ada da Silva et al. Antibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazon. Frontiers in Immunology, v. 10, p. 1-7, Sept. 2019. |
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https://www.arca.fiocruz.br/handle/icict/40286 |
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1664-3224 |
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10.3389/fimmu.2019.02230 |
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MATOS, Ada da Silva et al. Antibody Responses Against Plasmodium vivax TRAP Recombinant and Synthetic Antigens in Naturally Exposed Individuals From the Brazilian Amazon. Frontiers in Immunology, v. 10, p. 1-7, Sept. 2019. 1664-3224 10.3389/fimmu.2019.02230 |
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