Plasmodium vivax Cell-Traversal Protein for Ookinetes and Sporozoites: Naturally Acquired Humoral Immune Response and B-Cell Epitope Mapping in Brazilian Amazon Inhabitants
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/27097 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil. |
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Silva, Rodrigo Nunes Rodrigues daSoares, Isabela FerreiraLopez-Camacho, CesarSilva, João Hermínio Martins daSilva, Daiana de Souza Perce daTeva, AntonioFranco, Antônia Maria RamosPinheiro, Francimeire GomesChaves, Lana BitencourtPratt-Riccio, Lilian RoseReyes-Sandoval, ArturoBanic, Dalma MariaLima Junior, Josué da Costa2018-06-26T12:35:16Z2018-06-26T12:35:16Z2017SILVA, Rodrigo Nunes Rodrigues da; et al. Plasmodium vivax cell-Traversal Protein for Ookinetes and sporozoites: naturally acquired humoral immune response and B-cell epitope Mapping in Brazilian amazon inhabitants. Frontiers in Immunology, v.8, Article 77, 13p, Feb. 2017.1664-3224https://www.arca.fiocruz.br/handle/icict/2709710.3389/fimmu.2017.00077engFrontiers MediaMaláriaVacinasPlasmodium vivaxmapeamento de epitoposprevisão de epítopoAmazônia BrasileiraPvCelTOSP. vivaxvaccinesepitope mappingepitope predictionmalaria vaccinesmalariaPlasmodium vivax Cell-Traversal Protein for Ookinetes and Sporozoites: Naturally Acquired Humoral Immune Response and B-Cell Epitope Mapping in Brazilian Amazon Inhabitantsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.University of Oxford. The Henry Wellcome Building for Molecular Physiology. The Jenner Institute. Nuffield Department of Medicine. Oxford, UK.Fundação Oswaldo Cruz. Grupo de Modelagem Computacional. Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública Sergio Arouca. Departamento de Ciências Biológicas. Laboratório de Imunodiagnóstico. Rio de Janeiro, RJ, Brasil.Instituto Nacional de Pesquisas da Amazônia. Laboratório de Leishmanioses e Doença de Chagas. Manaus, AM, Brasil.Instituto Nacional de Pesquisas da Amazônia. Laboratório de Leishmanioses e Doença de Chagas. Manaus, AM, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ. Brasil.University of Oxford. The Henry Wellcome Building for Molecular Physiology. The Jenner Institute. Nuffield Department of Medicine. Oxford, UK.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Clínica. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil.The cell-traversal protein for ookinetes and sporozoites (CelTOS), a highly conserved antigen involved in sporozoite motility, plays an important role in the traversal of host cells during the preerythrocytic stage of Plasmodium species. Recently, it has been considered an alternative target when designing novel antimalarial vaccines against Plasmodium falciparum. However, the potential of Plasmodium vivax CelTOS as a vaccine target is yet to be explored. This study evaluated the naturally acquired immune response against a recombinant P. vivax CelTOS (PvCelTOS) (IgG and IgG subclass) in 528 individuals from Brazilian Amazon, as well as the screening of B-cell epitopes in silico and peptide assays to associate the breadth of antibody responses of those individuals with exposition and/or protection correlates. We show that PvCelTOS is naturally immunogenic in Amazon inhabitants with 94 individuals (17.8%) showing specific IgG antibodies against the recombinant protein. Among responders, the IgG reactivity indexes (RIs) presented a direct correlation with the number of previous malaria episodes (p = 0.003; r = 0.315) and inverse correlation with the time elapsed from the last malaria episode (p = 0.031; r = -0.258). Interestingly, high responders to PvCelTOS (RI > 2) presented higher number of previous malaria episodes, frequency of recent malaria episodes, and ratio of cytophilic/non-cytophilic antibodies than low responders (RI < 2) and non-responders (RI < 1). Moreover, a high prevalence of the cytophilic antibody IgG1 over all other IgG subclasses (p < 0.0001) was observed. B-cell epitope mapping revealed five immunogenic regions in PvCelTOS, but no associations between the specific IgG response to peptides and exposure/protection parameters were found. However, the epitope (PvCelTOSI136-E143) was validated as a main linear B-cell epitope, as 92% of IgG responders to PvCelTOS were also responders to this peptide sequence. This study describes for the first time the natural immunogenicity of PvCelTOS in Amazon individuals and identifies immunogenic regions in a full-length protein. The IgG magnitude was mainly composed of cytophilic antibodies (IgG1) and associated with recent malaria episodes. The data presented in this paper add further evidence to consider PvCelTOS as a vaccine candidate.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/27097/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALrodrigonunes_silva_etal_IOC_2017.pdfrodrigonunes_silva_etal_IOC_2017.pdfapplication/pdf1569700https://www.arca.fiocruz.br/bitstream/icict/27097/2/rodrigonunes_silva_etal_IOC_2017.pdf8641cdf40f5ed3ef3ea8dc91e56806d8MD52TEXTrodrigonunes_silva_etal_IOC_2017.pdf.txtrodrigonunes_silva_etal_IOC_2017.pdf.txtExtracted texttext/plain73640https://www.arca.fiocruz.br/bitstream/icict/27097/3/rodrigonunes_silva_etal_IOC_2017.pdf.txt49d28211d7e1435c6cc65f6cff1f2371MD53icict/270972022-06-24 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| dc.title.pt_BR.fl_str_mv |
Plasmodium vivax Cell-Traversal Protein for Ookinetes and Sporozoites: Naturally Acquired Humoral Immune Response and B-Cell Epitope Mapping in Brazilian Amazon Inhabitants |
| title |
Plasmodium vivax Cell-Traversal Protein for Ookinetes and Sporozoites: Naturally Acquired Humoral Immune Response and B-Cell Epitope Mapping in Brazilian Amazon Inhabitants |
| spellingShingle |
Plasmodium vivax Cell-Traversal Protein for Ookinetes and Sporozoites: Naturally Acquired Humoral Immune Response and B-Cell Epitope Mapping in Brazilian Amazon Inhabitants Silva, Rodrigo Nunes Rodrigues da Malária Vacinas Plasmodium vivax mapeamento de epitopos previsão de epítopo Amazônia Brasileira PvCelTOS P. vivax vaccines epitope mapping epitope prediction malaria vaccines malaria |
| title_short |
Plasmodium vivax Cell-Traversal Protein for Ookinetes and Sporozoites: Naturally Acquired Humoral Immune Response and B-Cell Epitope Mapping in Brazilian Amazon Inhabitants |
| title_full |
Plasmodium vivax Cell-Traversal Protein for Ookinetes and Sporozoites: Naturally Acquired Humoral Immune Response and B-Cell Epitope Mapping in Brazilian Amazon Inhabitants |
| title_fullStr |
Plasmodium vivax Cell-Traversal Protein for Ookinetes and Sporozoites: Naturally Acquired Humoral Immune Response and B-Cell Epitope Mapping in Brazilian Amazon Inhabitants |
| title_full_unstemmed |
Plasmodium vivax Cell-Traversal Protein for Ookinetes and Sporozoites: Naturally Acquired Humoral Immune Response and B-Cell Epitope Mapping in Brazilian Amazon Inhabitants |
| title_sort |
Plasmodium vivax Cell-Traversal Protein for Ookinetes and Sporozoites: Naturally Acquired Humoral Immune Response and B-Cell Epitope Mapping in Brazilian Amazon Inhabitants |
| author |
Silva, Rodrigo Nunes Rodrigues da |
| author_facet |
Silva, Rodrigo Nunes Rodrigues da Soares, Isabela Ferreira Lopez-Camacho, Cesar Silva, João Hermínio Martins da Silva, Daiana de Souza Perce da Teva, Antonio Franco, Antônia Maria Ramos Pinheiro, Francimeire Gomes Chaves, Lana Bitencourt Pratt-Riccio, Lilian Rose Reyes-Sandoval, Arturo Banic, Dalma Maria Lima Junior, Josué da Costa |
| author_role |
author |
| author2 |
Soares, Isabela Ferreira Lopez-Camacho, Cesar Silva, João Hermínio Martins da Silva, Daiana de Souza Perce da Teva, Antonio Franco, Antônia Maria Ramos Pinheiro, Francimeire Gomes Chaves, Lana Bitencourt Pratt-Riccio, Lilian Rose Reyes-Sandoval, Arturo Banic, Dalma Maria Lima Junior, Josué da Costa |
| author2_role |
author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Silva, Rodrigo Nunes Rodrigues da Soares, Isabela Ferreira Lopez-Camacho, Cesar Silva, João Hermínio Martins da Silva, Daiana de Souza Perce da Teva, Antonio Franco, Antônia Maria Ramos Pinheiro, Francimeire Gomes Chaves, Lana Bitencourt Pratt-Riccio, Lilian Rose Reyes-Sandoval, Arturo Banic, Dalma Maria Lima Junior, Josué da Costa |
| dc.subject.other.pt_BR.fl_str_mv |
Malária Vacinas Plasmodium vivax mapeamento de epitopos previsão de epítopo Amazônia Brasileira |
| topic |
Malária Vacinas Plasmodium vivax mapeamento de epitopos previsão de epítopo Amazônia Brasileira PvCelTOS P. vivax vaccines epitope mapping epitope prediction malaria vaccines malaria |
| dc.subject.en.pt_BR.fl_str_mv |
PvCelTOS P. vivax vaccines epitope mapping epitope prediction malaria vaccines malaria |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunoparasitologia. Rio de Janeiro, RJ. Brasil. |
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2017 |
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2017 |
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2018-06-26T12:35:16Z |
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2018-06-26T12:35:16Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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SILVA, Rodrigo Nunes Rodrigues da; et al. Plasmodium vivax cell-Traversal Protein for Ookinetes and sporozoites: naturally acquired humoral immune response and B-cell epitope Mapping in Brazilian amazon inhabitants. Frontiers in Immunology, v.8, Article 77, 13p, Feb. 2017. |
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https://www.arca.fiocruz.br/handle/icict/27097 |
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1664-3224 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fimmu.2017.00077 |
| identifier_str_mv |
SILVA, Rodrigo Nunes Rodrigues da; et al. Plasmodium vivax cell-Traversal Protein for Ookinetes and sporozoites: naturally acquired humoral immune response and B-cell epitope Mapping in Brazilian amazon inhabitants. Frontiers in Immunology, v.8, Article 77, 13p, Feb. 2017. 1664-3224 10.3389/fimmu.2017.00077 |
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Frontiers Media |
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Frontiers Media |
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