Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2007 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/39780 |
Resumo: | Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil. |
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Granthon, Ana ClaudiaBraga, Marina V.Rodrigues, Juliany C.F.Cammerer, SimonLorente, Silvia OrenesGilbert, Ian H.Urbina, Julio A.Souza, Wanderley de2020-02-06T15:57:27Z2020-02-06T15:57:27Z2007GRANTHON, Ana Claudia et al. Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors. Veterinary Parasitology, v. 146, p. 25-34, 2007.0304-4017https://www.arca.fiocruz.br/handle/icict/3978010.1016/j.vetpar.2006.12.0221873-2550engElsevierFarnesil-Difosfato FarnesiltransferaseLeishmaniaUltraestruturaQuimioterapiaErgosterolInibidores da biossíntese de esteróisLeishmaniaSqualene synthaseSterol biosynthesis inhibitorsErgosterolChemotherapyUltrastructureAlterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitorsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Biologia. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Cardiff University. Welsh School of Pharmacy. Cardiff, UK.Cardiff University. Welsh School of Pharmacy. Cardiff, UK.Cardiff University. Welsh School of Pharmacy. Cardiff, UK.Instituto Venezolano de Investigaciones Científicas. Centro de Biofísica y Bioquímica. Laboratorio de Química Biológica. Venezuela.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil.Leishmaniasis is an important disease in widely dispersed regions of the world. In South America, visceral leishmaniasis (VL) is mainly caused by Leishmania chagasi. The morbidity associated with the infection is high, and death may occur in some untreated patients. Treatment has been based upon pentavalent antimonial drugs for more than half a century and problems, including development of resistance to antimonials and lack of efficacy against VL/HIV co-infections, have emphasized the need for new drugs. Squalene synthase (SQS) is an essential enzyme for the biosynthesis of protozoal sterol molecules. In this work, nineteen synthetic quinuclidines, potentially inhibitors of SQS, were tested against promastigote forms of L. chagasi and the IC50 values of the compounds were determined. The most active compounds had IC50 values of around 30 nM and induced complete growth arrest and cell lysis at sub-micromolar concentrations. We analyzed the morphological structure of the parasites treated with these compounds by transmission electron microscopy of thin sections. Treated parasites showed significant ultrastructural changes, which varied from discrete alterations to total destruction of the cells, depending on the drug concentration and the time of incubation. One important change observed was a typical swelling of the unique and highly branched mitochondrion, where the inner membrane lost its organization. There was an increase in the number of autophagosomal structures. Changes in the organization of the nuclear chromatin and alterations in the flagellar pocket and flagellar membrane were also observed.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/39780/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarinaV_Braga_etal_IOC_2007.pdfMarinaV_Braga_etal_IOC_2007.pdfapplication/pdf1981690https://www.arca.fiocruz.br/bitstream/icict/39780/2/MarinaV_Braga_etal_IOC_2007.pdf5b63580d08742f8c85e5f36b9bd4e238MD52TEXTMarinaV_Braga_etal_IOC_2007.pdf.txtMarinaV_Braga_etal_IOC_2007.pdf.txtExtracted texttext/plain34462https://www.arca.fiocruz.br/bitstream/icict/39780/3/MarinaV_Braga_etal_IOC_2007.pdf.txt195e38618278b6cea8774118c1fee9e3MD53icict/397802020-02-07 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| dc.title.pt_BR.fl_str_mv |
Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors |
| title |
Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors |
| spellingShingle |
Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors Granthon, Ana Claudia Farnesil-Difosfato Farnesiltransferase Leishmania Ultraestrutura Quimioterapia Ergosterol Inibidores da biossíntese de esteróis Leishmania Squalene synthase Sterol biosynthesis inhibitors Ergosterol Chemotherapy Ultrastructure |
| title_short |
Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors |
| title_full |
Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors |
| title_fullStr |
Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors |
| title_full_unstemmed |
Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors |
| title_sort |
Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors |
| author |
Granthon, Ana Claudia |
| author_facet |
Granthon, Ana Claudia Braga, Marina V. Rodrigues, Juliany C.F. Cammerer, Simon Lorente, Silvia Orenes Gilbert, Ian H. Urbina, Julio A. Souza, Wanderley de |
| author_role |
author |
| author2 |
Braga, Marina V. Rodrigues, Juliany C.F. Cammerer, Simon Lorente, Silvia Orenes Gilbert, Ian H. Urbina, Julio A. Souza, Wanderley de |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Granthon, Ana Claudia Braga, Marina V. Rodrigues, Juliany C.F. Cammerer, Simon Lorente, Silvia Orenes Gilbert, Ian H. Urbina, Julio A. Souza, Wanderley de |
| dc.subject.mesh.pt_BR.fl_str_mv |
Farnesil-Difosfato Farnesiltransferase |
| topic |
Farnesil-Difosfato Farnesiltransferase Leishmania Ultraestrutura Quimioterapia Ergosterol Inibidores da biossíntese de esteróis Leishmania Squalene synthase Sterol biosynthesis inhibitors Ergosterol Chemotherapy Ultrastructure |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmania Ultraestrutura Quimioterapia Ergosterol Inibidores da biossíntese de esteróis |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmania Squalene synthase Sterol biosynthesis inhibitors Ergosterol Chemotherapy Ultrastructure |
| description |
Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Laboratório de Ultraestrutura Celular Hertha Meyer. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2007 |
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2007 |
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2020-02-06T15:57:27Z |
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2020-02-06T15:57:27Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
GRANTHON, Ana Claudia et al. Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors. Veterinary Parasitology, v. 146, p. 25-34, 2007. |
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https://www.arca.fiocruz.br/handle/icict/39780 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0304-4017 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.vetpar.2006.12.022 |
| dc.identifier.eissn.none.fl_str_mv |
1873-2550 |
| identifier_str_mv |
GRANTHON, Ana Claudia et al. Alterations on the growth and ultrastructure of Leishmania chagasi induced by squalene synthase inhibitors. Veterinary Parasitology, v. 146, p. 25-34, 2007. 0304-4017 10.1016/j.vetpar.2006.12.022 1873-2550 |
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Elsevier |
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Elsevier |
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