Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil

Detalhes bibliográficos
Autor(a) principal: Fukutani, Kiyoshi Ferreira
Data de Publicação: 2014
Outros Autores: Figueiredo, Virgínia, Celes, Fabiana Santana, Cristal, Juqueline Rocha, Barral, Aldina Maria Prado, Barral Netto, Manoel, Oliveira, Camila Indiani de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8408
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
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spelling Fukutani, Kiyoshi FerreiraFigueiredo, VirgíniaCeles, Fabiana SantanaCristal, Juqueline RochaBarral, Aldina Maria PradoBarral Netto, ManoelOliveira, Camila Indiani de2014-09-19T18:58:21Z2014-09-19T18:58:21Z2014FUKUTANI, K. F. et al. Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil. BMC Infectious Disease, v. 14, p. 422, 2014.1471-2334https://www.arca.fiocruz.br/handle/icict/840810.1186/1471-2334-14-422engBioMed CentralSerological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação de Hematologia e Hemoterapia da Bahia. HEMOBA/SESAB. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil / Instituto de Investigação em Imunologia. Salvador, BA, BrasilBACKGROUND: Visceral Leishmaniasis is endemic to Brazil, where it is caused by Leishmania infantum (syn. Leishmania chagasi). Following parasite inoculation, individuals may experience asymptomatic infection, raising the possibility of parasite transmission through the transfusion of contaminated blood products. In the present work, we evaluated the prevalence of asymptomatic Leishmania infection among blood donors in Salvador, northeastern Brazil. METHODS: Peripheral blood was collected from 700 blood donors attending the Blood Bank of Bahia (HEMOBA/SESAB), from January to September 2010. We evaluated anti-Leishmania serology by ELISA, employing Soluble Leishmania Antigen (sensitivity 90% and specificity 95%). The presence of parasite DNA was determined by qPCR, targeting a single copy gene (G6PD), and by end-point PCR, targeting multiple targets, namely a segment located in the Leishmania rRNA locus (ITS) and the conserved region of kinetoplastid DNA (kDNA) minicircles. RESULTS: The blood-donor population was comprised of 74.5% of males with a mean age of 34 years. Anti-Leishmania serology by ELISA was positive in 5.4% (38/700) individuals. One individual was also positive for Chagas' disease and another tested positive for Syphilis. Employing qPCR, parasite DNA was not found in any of 38 seropositive samples. However, by ITS PCR, 8/38 (21%) samples were positive and this positivity increased to 26/38 (68%) when we targeted kDNA amplification. Agreement between both techniques (ITS and kDNA PCR) was fair (kappa = 0.219). CONCLUSIONS: These results indicate that asymptomatic infection is present among the blood donor population of Salvador, a finding that warrants a broader discussion regarding the need to implement specific screening strategiesLeishmaniaBlood supplyAsymptomatic infectionPCRELISAinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8408/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALFukutani KF Serological....pdfFukutani KF Serological....pdfapplication/pdf882418https://www.arca.fiocruz.br/bitstream/icict/8408/2/Fukutani%20KF%20Serological....pdfd9ab719e76833ecc713e416c22ff3ee0MD52TEXTFukutani KF Serological....pdf.txtFukutani KF Serological....pdf.txtExtracted texttext/plain39095https://www.arca.fiocruz.br/bitstream/icict/8408/3/Fukutani%20KF%20Serological....pdf.txtcf7359c0ac223eeda3f0e1e53eb09574MD53icict/84082023-03-15 14:33:21.831oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil
title Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil
spellingShingle Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil
Fukutani, Kiyoshi Ferreira
Leishmania
Blood supply
Asymptomatic infection
PCR
ELISA
title_short Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil
title_full Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil
title_fullStr Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil
title_full_unstemmed Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil
title_sort Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil
author Fukutani, Kiyoshi Ferreira
author_facet Fukutani, Kiyoshi Ferreira
Figueiredo, Virgínia
Celes, Fabiana Santana
Cristal, Juqueline Rocha
Barral, Aldina Maria Prado
Barral Netto, Manoel
Oliveira, Camila Indiani de
author_role author
author2 Figueiredo, Virgínia
Celes, Fabiana Santana
Cristal, Juqueline Rocha
Barral, Aldina Maria Prado
Barral Netto, Manoel
Oliveira, Camila Indiani de
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Fukutani, Kiyoshi Ferreira
Figueiredo, Virgínia
Celes, Fabiana Santana
Cristal, Juqueline Rocha
Barral, Aldina Maria Prado
Barral Netto, Manoel
Oliveira, Camila Indiani de
dc.subject.en.pt_BR.fl_str_mv Leishmania
Blood supply
Asymptomatic infection
PCR
ELISA
topic Leishmania
Blood supply
Asymptomatic infection
PCR
ELISA
description Fundação Oswaldo Cruz. Centro de Pesquisa Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2014
dc.date.accessioned.fl_str_mv 2014-09-19T18:58:21Z
dc.date.available.fl_str_mv 2014-09-19T18:58:21Z
dc.date.issued.fl_str_mv 2014
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv FUKUTANI, K. F. et al. Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil. BMC Infectious Disease, v. 14, p. 422, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8408
dc.identifier.issn.none.fl_str_mv 1471-2334
dc.identifier.doi.none.fl_str_mv 10.1186/1471-2334-14-422
identifier_str_mv FUKUTANI, K. F. et al. Serological survey of Leishmania infection in blood donors in Salvador, Northeastern Brazil. BMC Infectious Disease, v. 14, p. 422, 2014.
1471-2334
10.1186/1471-2334-14-422
url https://www.arca.fiocruz.br/handle/icict/8408
dc.language.iso.fl_str_mv eng
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dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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