Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/47363 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. |
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Flores, Geane LopesBarbosa, Jakeline RibeiroCruz, Helena MedinaMiguel, Juliana CustódioPotsch, Denise VigoPilotto, José HenriqueLIma, Danielle MaltaColares, Jeová Keny BaimaMello, Carlos Eduardo BrandãoPires, Marcia Maria AmendolaMota, Marcia Maria AmendolaBastos, Francisco Inácio Pinkusfeld MonteiroLewis-Ximenez, Lia LauraVillar, Livia Melo2021-05-25T18:23:07Z2021-05-25T18:23:07Z2021FLORES, Geane Lopes et al. Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations World 3 Hepatol, v. 13, n. 4, p. 504-514, Apr. 2021.1948-5182https://www.arca.fiocruz.br/handle/icict/4736310.4254/wjh.v13.i4.504engBaishideng Publishing GroupEletroquimioluminescênciaMancha de sangue secoHepatite BHepatite CPopulações-chaveDiagnósticoDried blood spotElectrochemiluminescenceHepatitis BHepatitis CKey populationsDiagnosisDried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populationsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Universidade Federal do Ceará. Programa de Pós-Graduação em Patologia. Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Hospital Universitário Clementino Fraga Filho. Rio de Janeiro, RJ, Brasil.Hospital Geral de Nova Iguaçu. Nova Iguaçu, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade de Fortaleza. Programa de Pós-Graduação em Ciências Médicas. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Programa de Pós-Graduação em Patologia. Fortaleza, CE, Brasil.Universidade Federal do Rio de Janeiro. Escola de Medicina e Cirurgia. Hospital Universitário Gaffree&Guinle. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Escola de Medicina e Cirurgia. Hospital Universitário Gaffree&Guinle. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Comunicação e Informação em Ciência e Tecnologia em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Comunicação e Informação em Ciência e Tecnologia em Saúde. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil.BACKGROUND To achieve the elimination of hepatitis B and C, there is an urgent need to develop alternative strategies to increase the access of diagnosis, particularly among key populations such as people living with human immunodeficiency virus (HIV), individuals with coagulopathies and chronic kidney disease (CKD) patients. AIM To evaluate the use of dried blood spot (DBS) in the detection of hepatitis B virus (HBV) and hepatitis C virus (HCV) markers. METHODS A total of 430 individuals comprised of people living with HIV, coagulopathies and CKD provided paired serum and DBS samples. HBsAg, anti-HBc and anti-HCV were tested in those samples using a commercial electrochemiluminescence. Demographic and selected behavioral variables were evaluated to assess possible association with HBV and HCV positivity. RESULTS Using DBS, HBsAg prevalence varied from 3.9% to 22.1%, anti-HBc rates varied from 25.5% to 45.6% and anti-HCV positivity ranged from 15.9% to 41.2% in key populations. Specificities of HBV and HCV tests using DBS varied from 88.9% to 100%. The HBsAg assay demonstrated the best performance in CKD and coagulopathy individuals and the anti-HCV test had a sensitivity and specificity of 100% in people living with HIV. Accuracy of HBV and HCV detection in DBS varied from 90.2% to 100%. In the CKD group, HBsAg positivity was associated with infrequent use of condoms, and anti-HBc positivity was associated with sharing nail cutters/razors/toothbrushes. Anti-HCV reactivity was positively associated with a history of transplantation and length of time using hemodialysis in both specimens. In people living with HIV, only the male gender was associated with anti-HBc positivity in serum and DBS. CONCLUSION DBS with electrochemiluminescence are useful tools for the diagnosis and prevalence studies of hepatitis B and C among key populations and may increase the opportunity to foster prevention and treatment.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/47363/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALGeaneFlores_LiviaMVilar_etal_IOC_2021.pdfGeaneFlores_LiviaMVilar_etal_IOC_2021.pdfapplication/pdf502940https://www.arca.fiocruz.br/bitstream/icict/47363/2/GeaneFlores_LiviaMVilar_etal_IOC_2021.pdf4063008ddd8798c51c3ad709567ca753MD52TEXTGeaneFlores_LiviaMVilar_etal_IOC_2021.pdf.txtGeaneFlores_LiviaMVilar_etal_IOC_2021.pdf.txtExtracted 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| dc.title.pt_BR.fl_str_mv |
Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations |
| title |
Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations |
| spellingShingle |
Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations Flores, Geane Lopes Eletroquimioluminescência Mancha de sangue seco Hepatite B Hepatite C Populações-chave Diagnóstico Dried blood spot Electrochemiluminescence Hepatitis B Hepatitis C Key populations Diagnosis |
| title_short |
Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations |
| title_full |
Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations |
| title_fullStr |
Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations |
| title_full_unstemmed |
Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations |
| title_sort |
Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations |
| author |
Flores, Geane Lopes |
| author_facet |
Flores, Geane Lopes Barbosa, Jakeline Ribeiro Cruz, Helena Medina Miguel, Juliana Custódio Potsch, Denise Vigo Pilotto, José Henrique LIma, Danielle Malta Colares, Jeová Keny Baima Mello, Carlos Eduardo Brandão Pires, Marcia Maria Amendola Mota, Marcia Maria Amendola Bastos, Francisco Inácio Pinkusfeld Monteiro Lewis-Ximenez, Lia Laura Villar, Livia Melo |
| author_role |
author |
| author2 |
Barbosa, Jakeline Ribeiro Cruz, Helena Medina Miguel, Juliana Custódio Potsch, Denise Vigo Pilotto, José Henrique LIma, Danielle Malta Colares, Jeová Keny Baima Mello, Carlos Eduardo Brandão Pires, Marcia Maria Amendola Mota, Marcia Maria Amendola Bastos, Francisco Inácio Pinkusfeld Monteiro Lewis-Ximenez, Lia Laura Villar, Livia Melo |
| author2_role |
author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Flores, Geane Lopes Barbosa, Jakeline Ribeiro Cruz, Helena Medina Miguel, Juliana Custódio Potsch, Denise Vigo Pilotto, José Henrique LIma, Danielle Malta Colares, Jeová Keny Baima Mello, Carlos Eduardo Brandão Pires, Marcia Maria Amendola Mota, Marcia Maria Amendola Bastos, Francisco Inácio Pinkusfeld Monteiro Lewis-Ximenez, Lia Laura Villar, Livia Melo |
| dc.subject.other.pt_BR.fl_str_mv |
Eletroquimioluminescência Mancha de sangue seco Hepatite B Hepatite C Populações-chave Diagnóstico |
| topic |
Eletroquimioluminescência Mancha de sangue seco Hepatite B Hepatite C Populações-chave Diagnóstico Dried blood spot Electrochemiluminescence Hepatitis B Hepatitis C Key populations Diagnosis |
| dc.subject.en.pt_BR.fl_str_mv |
Dried blood spot Electrochemiluminescence Hepatitis B Hepatitis C Key populations Diagnosis |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatites Virais. Rio de Janeiro, RJ, Brasil. |
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2021 |
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2021-05-25T18:23:07Z |
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2021-05-25T18:23:07Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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FLORES, Geane Lopes et al. Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations World 3 Hepatol, v. 13, n. 4, p. 504-514, Apr. 2021. |
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https://www.arca.fiocruz.br/handle/icict/47363 |
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1948-5182 |
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10.4254/wjh.v13.i4.504 |
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FLORES, Geane Lopes et al. Dried blood spot sampling as an alternative for the improvement of hepatitis B and C diagnosis in key populations World 3 Hepatol, v. 13, n. 4, p. 504-514, Apr. 2021. 1948-5182 10.4254/wjh.v13.i4.504 |
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Baishideng Publishing Group |
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Baishideng Publishing Group |
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