Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection

Detalhes bibliográficos
Autor(a) principal: Bezerra, Cristianne Sousa
Data de Publicação: 2022
Outros Autores: Portilho, Moyra Machado, Barbosa, Jakeline Ribeiro, Azevedo, Carolina Pimentel de, Mendonça, Ana Carolina da Fonseca, Cruz, José Napoleão Monte da, Frota, Cristiane Cunha, Lago, Bárbara Vieira do, Villar, Lívia Melo
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/54656
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatitis Virais. Rio de Janeiro, RJ, Brasil / Instituto Federal de Educação, Ciência e Tecnologia do Ceará. Departamento de Educação. Fortaleza, CE, Brasil.
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spelling Bezerra, Cristianne SousaPortilho, Moyra MachadoBarbosa, Jakeline RibeiroAzevedo, Carolina Pimentel deMendonça, Ana Carolina da FonsecaCruz, José Napoleão Monte daFrota, Cristiane CunhaLago, Bárbara Vieira doVillar, Lívia Melo2022-08-16T13:23:22Z2022-08-16T13:23:22Z2022BEZERRA, Cristianne Sousa et al. Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection. Scientifc Reports, v. 12, 1651, p. 1 - 11, 2022.2045-2322https://www.arca.fiocruz.br/handle/icict/5465610.1038/s41598-022-05264-1engNature PortfolioAmostragem de sangue secoQuantificaçãoVírus da hepatite BSequenciamento e detecçãoMutaçõesDried blood spot samplingHepatitis B virusQuantificationSequencingMutation detectionDried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detectioninfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatitis Virais. Rio de Janeiro, RJ, Brasil / Instituto Federal de Educação, Ciência e Tecnologia do Ceará. Departamento de Educação. Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatitis Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatitis Virais. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatitis Virais. Rio de Janeiro, RJ, Brasil.Laboratório Central do Estado do Ceará. Fortaleza, CE, Brasil.Universidade Federal do Ceará. Faculade de Medicina. Departamento de Patologia e Medicina Legal. Fortaleza, CE, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatitis Virais. Rio de Janeiro, RJ, BrasilFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatitis Virais. Rio de Janeiro, RJ, BrasilHepatitis B virus (HBV) diagnosis is performed on serum samples, but the access to this diagnosis is difficult in low-income regions. The use of dried blood spot (DBS) samples does not require special structure for collection, storage or transport. This study evaluates the use of DBS for detection, quantification and sequencing of HBV DNA using in-house techniques. Two study groups were included: 92 HBsAg + individuals and 49 negative controls. Serum and DBS samples were submitted to quantitative and qualitative in-house PCR for S/pol genes, sequencing and phylogenetic analyses. Total of 84 serum samples were successfully amplified. Of them, 63 paired DBS were also positive in qualitative PCR. Qualitative PCR in DBS presented a sensitivity of 75% and specificity of 100% (Kappa = 0.689). Quantitative PCR in DBS presented a detection limit of 852.5 copies/mL (250 IU/mL), sensitivity of 77.63% and specificity of 100% (Kappa = 0.731). A total of 63 serum samples and 36 DBS samples were submitted to sequencing, revealing the circulation of genotypes A (65.08%), D (4.8%), E (3.2%) and F (27%) with 100% of correspondence between serum and DBS. All sequenced samples displayed polymorphisms in HBsAg gene. An HIV-coinfected patient presented the rtM204V/I-rtL180M double resistance mutation in serum and DBS. In conclusion, DBS is an alternative to detect, quantify and characterize HBV DNA, being a possibility of increasing diagnosis in low-income settings, closing gaps in HBV control.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/54656/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMoyraPortinho_LiviaVilar_etal_IOC_2022.pdfMoyraPortinho_LiviaVilar_etal_IOC_2022.pdfapplication/pdf1999889https://www.arca.fiocruz.br/bitstream/icict/54656/2/MoyraPortinho_LiviaVilar_etal_IOC_2022.pdfac1268f9b7096c383356ed16fdfcfdc7MD52icict/546562022-08-16 10:23:22.638oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-08-16T13:23:22Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection
title Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection
spellingShingle Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection
Bezerra, Cristianne Sousa
Amostragem de sangue seco
Quantificação
Vírus da hepatite B
Sequenciamento e detecção
Mutações
Dried blood spot sampling
Hepatitis B virus
Quantification
Sequencing
Mutation detection
title_short Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection
title_full Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection
title_fullStr Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection
title_full_unstemmed Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection
title_sort Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection
author Bezerra, Cristianne Sousa
author_facet Bezerra, Cristianne Sousa
Portilho, Moyra Machado
Barbosa, Jakeline Ribeiro
Azevedo, Carolina Pimentel de
Mendonça, Ana Carolina da Fonseca
Cruz, José Napoleão Monte da
Frota, Cristiane Cunha
Lago, Bárbara Vieira do
Villar, Lívia Melo
author_role author
author2 Portilho, Moyra Machado
Barbosa, Jakeline Ribeiro
Azevedo, Carolina Pimentel de
Mendonça, Ana Carolina da Fonseca
Cruz, José Napoleão Monte da
Frota, Cristiane Cunha
Lago, Bárbara Vieira do
Villar, Lívia Melo
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bezerra, Cristianne Sousa
Portilho, Moyra Machado
Barbosa, Jakeline Ribeiro
Azevedo, Carolina Pimentel de
Mendonça, Ana Carolina da Fonseca
Cruz, José Napoleão Monte da
Frota, Cristiane Cunha
Lago, Bárbara Vieira do
Villar, Lívia Melo
dc.subject.other.pt_BR.fl_str_mv Amostragem de sangue seco
Quantificação
Vírus da hepatite B
Sequenciamento e detecção
Mutações
topic Amostragem de sangue seco
Quantificação
Vírus da hepatite B
Sequenciamento e detecção
Mutações
Dried blood spot sampling
Hepatitis B virus
Quantification
Sequencing
Mutation detection
dc.subject.en.pt_BR.fl_str_mv Dried blood spot sampling
Hepatitis B virus
Quantification
Sequencing
Mutation detection
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Hepatitis Virais. Rio de Janeiro, RJ, Brasil / Instituto Federal de Educação, Ciência e Tecnologia do Ceará. Departamento de Educação. Fortaleza, CE, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-08-16T13:23:22Z
dc.date.available.fl_str_mv 2022-08-16T13:23:22Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BEZERRA, Cristianne Sousa et al. Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection. Scientifc Reports, v. 12, 1651, p. 1 - 11, 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/54656
dc.identifier.issn.pt_BR.fl_str_mv 2045-2322
dc.identifier.doi.none.fl_str_mv 10.1038/s41598-022-05264-1
identifier_str_mv BEZERRA, Cristianne Sousa et al. Dried blood spot sampling for hepatitis B virus quantification, sequencing and mutation detection. Scientifc Reports, v. 12, 1651, p. 1 - 11, 2022.
2045-2322
10.1038/s41598-022-05264-1
url https://www.arca.fiocruz.br/handle/icict/54656
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Nature Portfolio
publisher.none.fl_str_mv Nature Portfolio
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/54656/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/54656/2/MoyraPortinho_LiviaVilar_etal_IOC_2022.pdf
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MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
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