Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord

Detalhes bibliográficos
Autor(a) principal: Santos, Gisele Graça Leite dos
Data de Publicação: 2020
Outros Autores: Oliveira, Anna Lethícia Lima, Santos, Dourivaldo Silva, Espírito Santo, Renan Fernandes do, Silva, Daniela Nascimento, Juiz, Paulo José Lima, Soares, Milena Botelho Pereira, Villarreala, Cristiane Flora
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/46270
Resumo: FAPESB [grant number RED0010/2012].
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spelling Santos, Gisele Graça Leite dosOliveira, Anna Lethícia LimaSantos, Dourivaldo SilvaEspírito Santo, Renan Fernandes doSilva, Daniela NascimentoJuiz, Paulo José LimaSoares, Milena Botelho PereiraVillarreala, Cristiane Flora2021-03-05T13:45:34Z2021-03-05T13:45:34Z2020SANTOS, Gisele Graça Leite dos et al. Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord. Life Sciences, 2020.https://www.arca.fiocruz.br/handle/icict/46270FAPESB [grant number RED0010/2012].Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil.Federal University of Bahia. College of Pharmacy. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil.University Center SENAI/CIMATEC. SENAI Institute of Innovation in Advanced Health Systems. Salvador, BA, Brazil.Federal University of Recôncavo of Bahia. Feira de Santana, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / University Center SENAI/CIMATEC. SENAI Institute of Innovation in Advanced Health Systems. Salvador, BA, Brazil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Bahia, Brasil / Federal University of Bahia. College of Pharmacy. Salvador, BA, Brazil.The present study was designed to investigate whether the antinociceptive effect of bone marrow-derived mesenchymal stem/stromal cells (MSC) during oxaliplatin (OXL)-induced sensory neuropathy is related to antioxidant properties. Main methods: Male mice C57BL/6 were submitted to repeated intravenous administration of OXL (1 mg/kg, 9 administrations). After the establishment of sensory neuropathy, mice were treated with a single intravenous administration of MSC (1x106), vehicle or gabapentin. Paw mechanical and thermal nociceptive thresholds were evaluated through von Frey filaments and cold plate test, respectively. Motor performance was evaluated in the rotarod test. Gene expression profile, cytokine levels, and oxidative stress markers in the spinal cord were evaluated by real-time PCR, ELISA and biochemical assays, respectively. Key findings: OXL-treated mice presented behavioral signs of sensory neuropathy, such as mechanical allodynia and thermal hyperalgesia, which were completely reverted by a single administration of MSC. Repeated oral treatment with gabapentin (70 mg/kg) induced only transient antinociception. The IL-1β and TNF-α spinal levels did not differ between mice with or without sensory neuropathy. MSC increased the levels of anti-inflammatory cytokines, IL-10 and TGF-β, in the spinal cord of neuropathic mice, in addition to increasing the gene expression of antioxidant factors SOD and Nrf-2. Additionally, nitrite and MDA spinal levels were reduced by the MSC treatment. Significance: MSC induce reversion of sensory neuropathy induced by OXL possibly by activation of anti-inflammatory and antioxidant pathways, leading to reestablishment of redox homeostasis in the spinal cord.engElsevierCélulas Tronco MesenquimaisQuimioterapiaEstresse oxidativoCitocinasNeuropathic painChemotherapyMesenchymal CellsOxidative StressCytokinesMesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cordinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83097https://www.arca.fiocruz.br/bitstream/icict/46270/1/license.txt36b51ef91c52b5338d9d29ba0cc807bcMD51ORIGINALSantos, G.G.L Mesenchymal....pdfSantos, G.G.L 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dc.title.pt_BR.fl_str_mv Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord
title Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord
spellingShingle Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord
Santos, Gisele Graça Leite dos
Células Tronco Mesenquimais
Quimioterapia
Estresse oxidativo
Citocinas
Neuropathic pain
Chemotherapy
Mesenchymal Cells
Oxidative Stress
Cytokines
title_short Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord
title_full Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord
title_fullStr Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord
title_full_unstemmed Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord
title_sort Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord
author Santos, Gisele Graça Leite dos
author_facet Santos, Gisele Graça Leite dos
Oliveira, Anna Lethícia Lima
Santos, Dourivaldo Silva
Espírito Santo, Renan Fernandes do
Silva, Daniela Nascimento
Juiz, Paulo José Lima
Soares, Milena Botelho Pereira
Villarreala, Cristiane Flora
author_role author
author2 Oliveira, Anna Lethícia Lima
Santos, Dourivaldo Silva
Espírito Santo, Renan Fernandes do
Silva, Daniela Nascimento
Juiz, Paulo José Lima
Soares, Milena Botelho Pereira
Villarreala, Cristiane Flora
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Gisele Graça Leite dos
Oliveira, Anna Lethícia Lima
Santos, Dourivaldo Silva
Espírito Santo, Renan Fernandes do
Silva, Daniela Nascimento
Juiz, Paulo José Lima
Soares, Milena Botelho Pereira
Villarreala, Cristiane Flora
dc.subject.other.pt_BR.fl_str_mv Células Tronco Mesenquimais
Quimioterapia
Estresse oxidativo
Citocinas
topic Células Tronco Mesenquimais
Quimioterapia
Estresse oxidativo
Citocinas
Neuropathic pain
Chemotherapy
Mesenchymal Cells
Oxidative Stress
Cytokines
dc.subject.en.pt_BR.fl_str_mv Neuropathic pain
Chemotherapy
Mesenchymal Cells
Oxidative Stress
Cytokines
description FAPESB [grant number RED0010/2012].
publishDate 2020
dc.date.issued.fl_str_mv 2020
dc.date.accessioned.fl_str_mv 2021-03-05T13:45:34Z
dc.date.available.fl_str_mv 2021-03-05T13:45:34Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Gisele Graça Leite dos et al. Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord. Life Sciences, 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/46270
identifier_str_mv SANTOS, Gisele Graça Leite dos et al. Mesenchymal stem cells reduce the oxaliplatin-induced sensory neuropathy through the reestablishment of redox homeostasis in the spinal cord. Life Sciences, 2020.
url https://www.arca.fiocruz.br/handle/icict/46270
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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https://www.arca.fiocruz.br/bitstream/icict/46270/3/Santos%2c%20G.G.L%20Mesenchymal....pdf.txt
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repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
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