Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project

Detalhes bibliográficos
Autor(a) principal: Brandão, Adeilton
Data de Publicação: 1997
Outros Autores: Urmenyi, Turan, Rondinelli, Edson, Gonzalez, Antonio, Miranda, Antonio B. de, Degrave, Win
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/43902
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
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spelling Brandão, AdeiltonUrmenyi, TuranRondinelli, EdsonGonzalez, AntonioMiranda, Antonio B. deDegrave, Win2020-10-09T14:15:18Z2020-10-09T14:15:18Z1997BRANDÃO, Adeilton et al. Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 6, p. 863-866, Nov./Dec. 1997.0074-0276https://www.arca.fiocruz.br/handle/icict/4390210.1590/S0074-027619970006000241678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Sequenciamento de tags de sequência de sequência expressaBiblioteca cDNA normalizadaTrypanosoma cruziClone CL BrenerProjetos do Genoma do ParasitaExpressed sequence tags sequencingNormalized cDNA libraryTrypanosoma cruziClone CL BrenerParasite Genome ProjectsIdentification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Projectinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro. Instituto de Biofísica Carlos Chagas Filho. Rio de Janeiro, RJ, Brasil.Instituto de Parasitologia e Biomedicina, Calle Ventanilla. Granada, Spain.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Random single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and forms an integral part of most genome projects, with the objective to identify new genes and targets for disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/43902/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALAdeilton_Brandao_etal_IOC_1997.pdfAdeilton_Brandao_etal_IOC_1997.pdfapplication/pdf71000https://www.arca.fiocruz.br/bitstream/icict/43902/2/Adeilton_Brandao_etal_IOC_1997.pdfff4c9e2cf6cbe30a79f27cd33eefcf1dMD52TEXTAdeilton_Brandao_etal_IOC_1997.pdf.txtAdeilton_Brandao_etal_IOC_1997.pdf.txtExtracted texttext/plain16018https://www.arca.fiocruz.br/bitstream/icict/43902/3/Adeilton_Brandao_etal_IOC_1997.pdf.txt7b80f012d2b4763ac62f6c27a3ad8e4cMD53icict/439022020-10-10 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dc.title.pt_BR.fl_str_mv Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
title Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
spellingShingle Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
Brandão, Adeilton
Sequenciamento de tags de sequência de sequência expressa
Biblioteca cDNA normalizada
Trypanosoma cruzi
Clone CL Brener
Projetos do Genoma do Parasita
Expressed sequence tags sequencing
Normalized cDNA library
Trypanosoma cruzi
Clone CL Brener
Parasite Genome Projects
title_short Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
title_full Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
title_fullStr Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
title_full_unstemmed Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
title_sort Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
author Brandão, Adeilton
author_facet Brandão, Adeilton
Urmenyi, Turan
Rondinelli, Edson
Gonzalez, Antonio
Miranda, Antonio B. de
Degrave, Win
author_role author
author2 Urmenyi, Turan
Rondinelli, Edson
Gonzalez, Antonio
Miranda, Antonio B. de
Degrave, Win
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Brandão, Adeilton
Urmenyi, Turan
Rondinelli, Edson
Gonzalez, Antonio
Miranda, Antonio B. de
Degrave, Win
dc.subject.other.pt_BR.fl_str_mv Sequenciamento de tags de sequência de sequência expressa
Biblioteca cDNA normalizada
Trypanosoma cruzi
Clone CL Brener
Projetos do Genoma do Parasita
topic Sequenciamento de tags de sequência de sequência expressa
Biblioteca cDNA normalizada
Trypanosoma cruzi
Clone CL Brener
Projetos do Genoma do Parasita
Expressed sequence tags sequencing
Normalized cDNA library
Trypanosoma cruzi
Clone CL Brener
Parasite Genome Projects
dc.subject.en.pt_BR.fl_str_mv Expressed sequence tags sequencing
Normalized cDNA library
Trypanosoma cruzi
Clone CL Brener
Parasite Genome Projects
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.
publishDate 1997
dc.date.issued.fl_str_mv 1997
dc.date.accessioned.fl_str_mv 2020-10-09T14:15:18Z
dc.date.available.fl_str_mv 2020-10-09T14:15:18Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BRANDÃO, Adeilton et al. Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 6, p. 863-866, Nov./Dec. 1997.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/43902
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02761997000600024
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv BRANDÃO, Adeilton et al. Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 92, n. 6, p. 863-866, Nov./Dec. 1997.
0074-0276
10.1590/S0074-02761997000600024
1678-8060
url https://www.arca.fiocruz.br/handle/icict/43902
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
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