Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project

Detalhes bibliográficos
Autor(a) principal: Brandão,Adeílton
Data de Publicação: 1997
Outros Autores: Urmenyi,Turan, Rondinelli,Edson, Gonzalez,Antonio, Miranda,Antonio B de, Degrave,Wim
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600024
Resumo: Random single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and forms an integral part of most genome projects, with the objective to identify new genes and targets for disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi.
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spelling Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Projectexpressed sequence tags sequencingnormalized cDNA libraryTrypanosoma cruziclone CL BrenerParasite Genome ProjectsRandom single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and forms an integral part of most genome projects, with the objective to identify new genes and targets for disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi.Instituto Oswaldo Cruz, Ministério da Saúde1997-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600024Memórias do Instituto Oswaldo Cruz v.92 n.6 1997reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02761997000600024info:eu-repo/semantics/openAccessBrandão,AdeíltonUrmenyi,TuranRondinelli,EdsonGonzalez,AntonioMiranda,Antonio B deDegrave,Wimeng2020-04-25T17:47:46Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:07:45.056Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
title Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
spellingShingle Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
Brandão,Adeílton
expressed sequence tags sequencing
normalized cDNA library
Trypanosoma cruzi
clone CL Brener
Parasite Genome Projects
title_short Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
title_full Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
title_fullStr Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
title_full_unstemmed Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
title_sort Identification of Transcribed Sequences (ESTs) in the Trypanosoma cruzi Genome Project
author Brandão,Adeílton
author_facet Brandão,Adeílton
Urmenyi,Turan
Rondinelli,Edson
Gonzalez,Antonio
Miranda,Antonio B de
Degrave,Wim
author_role author
author2 Urmenyi,Turan
Rondinelli,Edson
Gonzalez,Antonio
Miranda,Antonio B de
Degrave,Wim
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Brandão,Adeílton
Urmenyi,Turan
Rondinelli,Edson
Gonzalez,Antonio
Miranda,Antonio B de
Degrave,Wim
dc.subject.por.fl_str_mv expressed sequence tags sequencing
normalized cDNA library
Trypanosoma cruzi
clone CL Brener
Parasite Genome Projects
topic expressed sequence tags sequencing
normalized cDNA library
Trypanosoma cruzi
clone CL Brener
Parasite Genome Projects
dc.description.none.fl_txt_mv Random single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and forms an integral part of most genome projects, with the objective to identify new genes and targets for disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi.
description Random single pass sequencing of cDNA fragments, also known as generation of Expressed Sequence Tags (ESTs), has been highly successful in the study of the gene content of higher organisms, and forms an integral part of most genome projects, with the objective to identify new genes and targets for disease control and prevention and to generate mapping probes. In the Trypanosoma cruzi genome project, EST sequencing has also been a starting point, and here we report data on the first 797 sequences obtained, partly from a CL Brener epimastigote non-normalized library, partly on a normalized library. Only around 30% of the sequences obtained showed similarity with Genbank and dbEST databases, half of which with sequences already reported for T. cruzi.
publishDate 1997
dc.date.none.fl_str_mv 1997-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600024
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02761997000600024
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02761997000600024
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.92 n.6 1997
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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