Multiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiology
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/40578 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. |
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Raposo, Jéssica VasquesAlves, Arthur Daniel RochaSilva, Alexandre dos Santos daSantos, Damião Carlos dosMelgaço, Juliana GilMoreira, Otacilio C.Pinto, Marcelo AlvesPaula, Vanessa Salete de2020-03-31T17:27:33Z2020-03-31T17:27:33Z2019RAPOSO, Jéssica Vasques et al. Multiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiology. BMC Infectious Diseases, v. 19, n. 773, p. 1-8, 2019.1471-2334https://www.arca.fiocruz.br/handle/icict/4057810.1186/s12879-019-4309-4engBMCVírus betaherpesInsuficiência hepática agudaMultiplexFígadoReação em Cadeia da Polimerase MultiplexBetaherpesvirusesAcute liver failureqPCRMultiplexLiverMultiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiologyinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Universidade Estácio de Sá. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Desenvolvimento Tecnológico em Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil.Background: The etiology of acute liver failure (ALF) is often unknown and reported to be associated with herpesviruses in a number of cases. In this study, we examined for betaherpesviruses infections in patients with ALF of unknown etiology using a multiplex qPCR to Betaherpesviruses subfamily. Methods: Liver explant and serum samples from 27 patients with ALF of unknown etiology were analyzed with the aid of multiplex qPCR to identify betaherpesviruses. All positive samples were sequenced to confirm herpes infection and liver enzyme levels evaluated. Results: Betaherpesviruses infection was effectively detected using multiplex qPCR. Six (22%) HHV-6, one (3%) HCMV and two (7%) dual infections (one with HHV-7/HHV-6, and the other with HHV-7/ HCMV). Interestingly, HHV-7 was only detected in the presence of other betaherpesviruses. Sequencing information confirmed betaherpesviruses infection. High hepatic enzyme levels and INR values> 1.5 were determined in all betaherpesvirus-positive patients. Conclusions: Multiplex qPCR facilitated efficient quantification, indicating that differentiation between betaherpesviruses is possible with the sole use of real-time PCR. Liver explant and serum samples were positive for some betaherpesviruses, and coinfection of HHV-7 with HHV-6 and HCMV was additionally detected. Based on these results, we propose that ALF patients should be screened for the presence of betaherpesviruses.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/40578/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarceloaPInto_AlexandreSSIlva_etal_IOC_2019.pdfMarceloaPInto_AlexandreSSIlva_etal_IOC_2019.pdfapplication/pdf744272https://www.arca.fiocruz.br/bitstream/icict/40578/2/MarceloaPInto_AlexandreSSIlva_etal_IOC_2019.pdfbbbd21aad3201612096a1ed21f5cba36MD52TEXTMarceloaPInto_AlexandreSSIlva_etal_IOC_2019.pdf.txtMarceloaPInto_AlexandreSSIlva_etal_IOC_2019.pdf.txtExtracted texttext/plain36174https://www.arca.fiocruz.br/bitstream/icict/40578/3/MarceloaPInto_AlexandreSSIlva_etal_IOC_2019.pdf.txt2f4d845844ae23f174a6a349feb0b7a1MD53icict/405782022-06-24 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| dc.title.pt_BR.fl_str_mv |
Multiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiology |
| title |
Multiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiology |
| spellingShingle |
Multiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiology Raposo, Jéssica Vasques Vírus betaherpes Insuficiência hepática aguda Multiplex Fígado Reação em Cadeia da Polimerase Multiplex Betaherpesviruses Acute liver failure qPCR Multiplex Liver |
| title_short |
Multiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiology |
| title_full |
Multiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiology |
| title_fullStr |
Multiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiology |
| title_full_unstemmed |
Multiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiology |
| title_sort |
Multiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiology |
| author |
Raposo, Jéssica Vasques |
| author_facet |
Raposo, Jéssica Vasques Alves, Arthur Daniel Rocha Silva, Alexandre dos Santos da Santos, Damião Carlos dos Melgaço, Juliana Gil Moreira, Otacilio C. Pinto, Marcelo Alves Paula, Vanessa Salete de |
| author_role |
author |
| author2 |
Alves, Arthur Daniel Rocha Silva, Alexandre dos Santos da Santos, Damião Carlos dos Melgaço, Juliana Gil Moreira, Otacilio C. Pinto, Marcelo Alves Paula, Vanessa Salete de |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Raposo, Jéssica Vasques Alves, Arthur Daniel Rocha Silva, Alexandre dos Santos da Santos, Damião Carlos dos Melgaço, Juliana Gil Moreira, Otacilio C. Pinto, Marcelo Alves Paula, Vanessa Salete de |
| dc.subject.other.pt_BR.fl_str_mv |
Vírus betaherpes Insuficiência hepática aguda Multiplex Fígado Reação em Cadeia da Polimerase Multiplex |
| topic |
Vírus betaherpes Insuficiência hepática aguda Multiplex Fígado Reação em Cadeia da Polimerase Multiplex Betaherpesviruses Acute liver failure qPCR Multiplex Liver |
| dc.subject.en.pt_BR.fl_str_mv |
Betaherpesviruses Acute liver failure qPCR Multiplex Liver |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Molecular. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2019 |
| dc.date.issued.fl_str_mv |
2019 |
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2020-03-31T17:27:33Z |
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2020-03-31T17:27:33Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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RAPOSO, Jéssica Vasques et al. Multiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiology. BMC Infectious Diseases, v. 19, n. 773, p. 1-8, 2019. |
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https://www.arca.fiocruz.br/handle/icict/40578 |
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1471-2334 |
| dc.identifier.doi.none.fl_str_mv |
10.1186/s12879-019-4309-4 |
| identifier_str_mv |
RAPOSO, Jéssica Vasques et al. Multiplex qPCR facilitates identification of betaherpesviruses in patients with acute liver failure of unknown etiology. BMC Infectious Diseases, v. 19, n. 773, p. 1-8, 2019. 1471-2334 10.1186/s12879-019-4309-4 |
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BMC |
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