Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/56487 |
Resumo: | Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil. |
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Mendonça, Lucilla Silva OliveiraMendes, Érica AraújoCastro, Julyanna OliveiraSilva, Mylene MeloSantos, Andréa GonçalvesKaneto, Carla MartinsDias, Sandro OliveiraAllaman, Ivan BezerraVannier-Santos, Marcos AndréSilva, Juneo FreitasAugusto, Danillo GardenalAnjos, Danielle Oliveira dosSantos, Nailma Aprigio SilvaLima, Kamila PontesHorta, Maria de FátimaAlbuquerque, George RegoCosta, Márcio Gilberto CardosoSilva-Jardim, IzaltinaSantos, Jane Lima dos2023-01-17T19:23:31Z2023-01-17T19:23:31Z2022MENDONÇA, Lucilla Silva Oliveira et al. Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages. Current Research in Microbial Sciences, v. 3, 100145, p. 1 - 3, June 2022.2666-5174https://www.arca.fiocruz.br/handle/icict/5648710.1016/j.crmicr.2022.100145engElsevier B.V.BiofungicidamicroRNAs TrichodermaAutofagiaROSInflamassomaBiofungicideTrichoderma microRNAsAutophagyROSInflammasomesTrichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophagesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil.Universidade de São Paulo. Instituto de Ciências Biológicas. Departamento de Microbiologia. São Paulo, SP, Brasil.êUniversidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Exatas e Tecnológicas. Santa Cruz, BA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Inovações em Terapias, Ensino e Bioprodutos. Rio de Janeiro, RJ, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil.Universidade Federal do Paraná. Laboratório de Genética Molecular Humana. Curitiba, PR, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil / Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil.Instituto de Ciências Biológicas. Departamento de Bioquímica e Imunologia. Belo Horizonte, MG, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Agrárias e Ambientais. Ilhéus, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil.Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil.Trichoderma spp. are usually considered safe and normally used as biocontrol and biofertilization. Safety for human health is evaluated by several tests that detect various effects such as allergenicity, toxicity, infectivity, and pathogenicity. However, they do not evaluate the effects of the agent upon the immune system. The aim of this study was to investigate the interaction between T. stromaticum spores and mammalian cells to assess the immunomodulatory potential of the spores of this fungus. First, mouse macrophage cell line J774 and human macrophages were exposed to fungal spores and analyzed for structural features, through scanning and transmission electron microscopy. Then, various analysis were performed in human macrophages as to their effect in some functional and molecular aspects of the immune system through immunocytochemistry, flow cytometry and gene expression assays. We demonstrated that T. stromaticum spores induces autophagy and autophagyrelated genes (ATGs) and downmodulate inflammatory mediators, including ROS, NLRP3, the cytokines IL-1β, IL-18, IL-12 and IL-10, as well as TLR2, TLR4, miR-146b and miR-155, which may lead to an augmented susceptibility to pathogens. Our study shows the extension of damages the biofungicide Tricovab® can cause in the innate immune response. Further studies are necessary to elucidate other innate and adaptive immune responses and, consequently, the safety of this fungus when in contact with humans.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/56487/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALMarcosAndreVSantos_etal_IOC_2022.pdfMarcosAndreVSantos_etal_IOC_2022.pdfapplication/pdf8683569https://www.arca.fiocruz.br/bitstream/icict/56487/2/MarcosAndreVSantos_etal_IOC_2022.pdf269afb310ca78dcef1e35d3f4bd83216MD52icict/564872023-09-14 16:02:24.554oai:www.arca.fiocruz.br:icict/56487Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-09-14T19:02:24Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.en_US.fl_str_mv |
Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages |
| title |
Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages |
| spellingShingle |
Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages Mendonça, Lucilla Silva Oliveira Biofungicida microRNAs Trichoderma Autofagia ROS Inflamassoma Biofungicide Trichoderma microRNAs Autophagy ROS Inflammasomes |
| title_short |
Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages |
| title_full |
Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages |
| title_fullStr |
Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages |
| title_full_unstemmed |
Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages |
| title_sort |
Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages |
| author |
Mendonça, Lucilla Silva Oliveira |
| author_facet |
Mendonça, Lucilla Silva Oliveira Mendes, Érica Araújo Castro, Julyanna Oliveira Silva, Mylene Melo Santos, Andréa Gonçalves Kaneto, Carla Martins Dias, Sandro Oliveira Allaman, Ivan Bezerra Vannier-Santos, Marcos André Silva, Juneo Freitas Augusto, Danillo Gardenal Anjos, Danielle Oliveira dos Santos, Nailma Aprigio Silva Lima, Kamila Pontes Horta, Maria de Fátima Albuquerque, George Rego Costa, Márcio Gilberto Cardoso Silva-Jardim, Izaltina Santos, Jane Lima dos |
| author_role |
author |
| author2 |
Mendes, Érica Araújo Castro, Julyanna Oliveira Silva, Mylene Melo Santos, Andréa Gonçalves Kaneto, Carla Martins Dias, Sandro Oliveira Allaman, Ivan Bezerra Vannier-Santos, Marcos André Silva, Juneo Freitas Augusto, Danillo Gardenal Anjos, Danielle Oliveira dos Santos, Nailma Aprigio Silva Lima, Kamila Pontes Horta, Maria de Fátima Albuquerque, George Rego Costa, Márcio Gilberto Cardoso Silva-Jardim, Izaltina Santos, Jane Lima dos |
| author2_role |
author author author author author author author author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Mendonça, Lucilla Silva Oliveira Mendes, Érica Araújo Castro, Julyanna Oliveira Silva, Mylene Melo Santos, Andréa Gonçalves Kaneto, Carla Martins Dias, Sandro Oliveira Allaman, Ivan Bezerra Vannier-Santos, Marcos André Silva, Juneo Freitas Augusto, Danillo Gardenal Anjos, Danielle Oliveira dos Santos, Nailma Aprigio Silva Lima, Kamila Pontes Horta, Maria de Fátima Albuquerque, George Rego Costa, Márcio Gilberto Cardoso Silva-Jardim, Izaltina Santos, Jane Lima dos |
| dc.subject.other.en_US.fl_str_mv |
Biofungicida microRNAs Trichoderma Autofagia ROS Inflamassoma |
| topic |
Biofungicida microRNAs Trichoderma Autofagia ROS Inflamassoma Biofungicide Trichoderma microRNAs Autophagy ROS Inflammasomes |
| dc.subject.en.en_US.fl_str_mv |
Biofungicide Trichoderma microRNAs Autophagy ROS Inflammasomes |
| description |
Universidade Estadual de Santa Cruz. Departamento de Ciências Biológicas. Ilhéus, BA, Brasil. |
| publishDate |
2022 |
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2022 |
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2023-01-17T19:23:31Z |
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2023-01-17T19:23:31Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
MENDONÇA, Lucilla Silva Oliveira et al. Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages. Current Research in Microbial Sciences, v. 3, 100145, p. 1 - 3, June 2022. |
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https://www.arca.fiocruz.br/handle/icict/56487 |
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2666-5174 |
| dc.identifier.doi.none.fl_str_mv |
10.1016/j.crmicr.2022.100145 |
| identifier_str_mv |
MENDONÇA, Lucilla Silva Oliveira et al. Trichoderma stromaticum spores induce autophagy and downregulate inflammatory mediators in human peripheral blood-derived macrophages. Current Research in Microbial Sciences, v. 3, 100145, p. 1 - 3, June 2022. 2666-5174 10.1016/j.crmicr.2022.100145 |
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Elsevier B.V. |
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Elsevier B.V. |
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