Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis

Detalhes bibliográficos
Autor(a) principal: Souza, Daniel Menezes
Data de Publicação: 2012
Outros Autores: Sá, Renata Guerra, Carneiro, Cláudia Martins, Souza, Juliana Vitoriano, Giunchetti, Rodolfo Cordeiro, Carvalho, Andréa Teixeira de, Lemos, Denise da Silveira, Oliveira, Guilherme Corrêa de, Oliveira, Rodrigo Corrêa de, Reis, Alexandre Barbosa
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/7763
Resumo: Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular.. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Bioquímica e Biologia Molecular. Ouro Preto, MG, Brasil
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spelling Souza, Daniel MenezesSá, Renata GuerraCarneiro, Cláudia MartinsSouza, Juliana VitorianoGiunchetti, Rodolfo CordeiroCarvalho, Andréa Teixeira deLemos, Denise da SilveiraOliveira, Guilherme Corrêa deOliveira, Rodrigo Corrêa deReis, Alexandre Barbosa2014-06-03T19:00:00Z2014-06-03T19:00:00Z2012SOUZA, Daniel Menezes et al. Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis. Plos Neglected Tropical Disease. 2012, vol.6, n.4, pp. e1566.https://www.arca.fiocruz.br/handle/icict/776310.1371/journal.pntd.0001566engPublice Science LibraryHigher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular.. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Bioquímica e Biologia Molecular. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Bioquímica e Biologia Molecular. Ouro Preto, MG, Brasil/Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Ciências Biológicas. Ouro Preto, Minas Gerais, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Instituto de Ciências Exatas e Biológicas. Departamento de Ciências Biológicas. Ouro Preto, Minas Gerais, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Biomarcadores de Diagnóstico e Monitoração. Belo Horizonte, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunoparasitologia. Ouro Preto, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Parasitologia Celular e Molecular. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, BrasilUniversidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Escola de Farmácia. Departamento de Análises Clínicas. Ouro Preto, MG, BrasilBackground The immune response in the skin of dogs infected with Leishmania infantum is poorly understood, and limited studies have described the immunopathological profile with regard to distinct levels of tissue parasitism and the clinical progression of canine visceral leishmaniasis (CVL). Methodology/Principal Findings A detailed analysis of inflammatory cells (neutrophils, eosinophils, mast cells, lymphocytes, and macrophages) as well as the expression of chemokines (CCL2, CCL4, CCL5, CCL13, CCL17, CCL21, CCL24, and CXCL8) was carried out in dermis skin samples from 35 dogs that were naturally infected with L. infantum. The analysis was based on real-time polymerase chain reaction (PCR) in the context of skin parasitism and the clinical status of CVL. We demonstrated increased inflammatory infiltrate composed mainly of mononuclear cells in the skin of animals with severe forms of CVL and high parasite density. Analysis of the inflammatory cell profile of the skin revealed an increase in the number of macrophages and reductions in lymphocytes, eosinophils, and mast cells that correlated with clinical progression of the disease. Additionally, enhanced parasite density was correlated with an increase in macrophages and decreases in eosinophils and mast cells. The chemokine mRNA expression demonstrated that enhanced parasite density was positively correlated with the expression of CCL2, CCL4, CCL5, CCL21, and CXCL8. In contrast, there was a negative correlation between parasite density and CCL24 expression. Conclusions/Significance These findings represent an advance in the knowledge about skin inflammatory infiltrates in CVL and the systemic consequences. Additionally, the findings may contribute to the design of new and more efficient prophylactic tools and immunological therapies against CVL.ChemokinesDogsInflammationLeishmaniaLeishmania infantumMacrophagesParasitic diseasesSkin infectionsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALHigher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated .pdfHigher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated .pdfapplication/pdf2662918https://www.arca.fiocruz.br/bitstream/icict/7763/1/Higher%20expression%20of%20CCL2%2c%20CCL4%2c%20CCL5%2c%20CCL21%2c%20and%20CXCL8%20chemokines%20in%20the%20skin%20associated%20.pdff9a79bde761bca0ecb26ddb736cb32aaMD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/7763/2/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD52TEXTHigher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated .pdf.txtHigher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated .pdf.txtExtracted texttext/plain11https://www.arca.fiocruz.br/bitstream/icict/7763/5/Higher%20expression%20of%20CCL2%2c%20CCL4%2c%20CCL5%2c%20CCL21%2c%20and%20CXCL8%20chemokines%20in%20the%20skin%20associated%20.pdf.txtb308b7fe5c1c2bbdc0cb686d451b84aaMD55THUMBNAILHigher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated .pdf.jpgHigher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated .pdf.jpgGenerated Thumbnailimage/jpeg2242https://www.arca.fiocruz.br/bitstream/icict/7763/4/Higher%20expression%20of%20CCL2%2c%20CCL4%2c%20CCL5%2c%20CCL21%2c%20and%20CXCL8%20chemokines%20in%20the%20skin%20associated%20.pdf.jpg595f238d37ac521fd6dbdd452850c7ebMD54icict/77632018-04-06 08:48:43.678oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352018-04-06T11:48:43Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis
title Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis
spellingShingle Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis
Souza, Daniel Menezes
Chemokines
Dogs
Inflammation
Leishmania
Leishmania infantum
Macrophages
Parasitic diseases
Skin infections
title_short Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis
title_full Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis
title_fullStr Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis
title_full_unstemmed Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis
title_sort Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis
author Souza, Daniel Menezes
author_facet Souza, Daniel Menezes
Sá, Renata Guerra
Carneiro, Cláudia Martins
Souza, Juliana Vitoriano
Giunchetti, Rodolfo Cordeiro
Carvalho, Andréa Teixeira de
Lemos, Denise da Silveira
Oliveira, Guilherme Corrêa de
Oliveira, Rodrigo Corrêa de
Reis, Alexandre Barbosa
author_role author
author2 Sá, Renata Guerra
Carneiro, Cláudia Martins
Souza, Juliana Vitoriano
Giunchetti, Rodolfo Cordeiro
Carvalho, Andréa Teixeira de
Lemos, Denise da Silveira
Oliveira, Guilherme Corrêa de
Oliveira, Rodrigo Corrêa de
Reis, Alexandre Barbosa
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Souza, Daniel Menezes
Sá, Renata Guerra
Carneiro, Cláudia Martins
Souza, Juliana Vitoriano
Giunchetti, Rodolfo Cordeiro
Carvalho, Andréa Teixeira de
Lemos, Denise da Silveira
Oliveira, Guilherme Corrêa de
Oliveira, Rodrigo Corrêa de
Reis, Alexandre Barbosa
dc.subject.en.pt_BR.fl_str_mv Chemokines
Dogs
Inflammation
Leishmania
Leishmania infantum
Macrophages
Parasitic diseases
Skin infections
topic Chemokines
Dogs
Inflammation
Leishmania
Leishmania infantum
Macrophages
Parasitic diseases
Skin infections
description Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Imunopatologia. Ouro Preto, MG, Brasil/Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Imunologia Celular e Molecular.. Belo Horizonte, MG, Brasil/Universidade Federal de Ouro Preto. Núcleo de Pesquisas em Ciências Biológicas. Laboratório de Bioquímica e Biologia Molecular. Ouro Preto, MG, Brasil
publishDate 2012
dc.date.issued.fl_str_mv 2012
dc.date.accessioned.fl_str_mv 2014-06-03T19:00:00Z
dc.date.available.fl_str_mv 2014-06-03T19:00:00Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SOUZA, Daniel Menezes et al. Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis. Plos Neglected Tropical Disease. 2012, vol.6, n.4, pp. e1566.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/7763
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pntd.0001566
identifier_str_mv SOUZA, Daniel Menezes et al. Higher expression of CCL2, CCL4, CCL5, CCL21, and CXCL8 chemokines in the skin associated with parasite density in canine visceral leishmaniasis. Plos Neglected Tropical Disease. 2012, vol.6, n.4, pp. e1566.
10.1371/journal.pntd.0001566
url https://www.arca.fiocruz.br/handle/icict/7763
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Publice Science Library
publisher.none.fl_str_mv Publice Science Library
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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