Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment

Detalhes bibliográficos
Autor(a) principal: Santana, Marlon G. Veloso de
Data de Publicação: 2014
Outros Autores: Neves, Patrícia C. C., Santos, Juliana Ribeiro dos, Lima, Noemia S., Santos, Alexandre A. C. dos, Watkins, David I., Galler, Ricardo, Ronaldo, Myrna C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10203
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil / University of Miami. Miller School of Medicine. Department of Pathology. Miami, USA.
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spelling Santana, Marlon G. Veloso deNeves, Patrícia C. C.Santos, Juliana Ribeiro dosLima, Noemia S.Santos, Alexandre A. C. dosWatkins, David I.Galler, RicardoRonaldo, Myrna C.2015-05-04T17:07:29Z2015-05-04T17:07:29Z2014SANTANA, Marlon G. Veloso et al. Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment. Virology, n. 452-453, p. 202–211, 2014.1743-422Xhttps://www.arca.fiocruz.br/handle/icict/1020310.1016/j.virol.2014.01.017engElsevierFebre AmarelaYellow Fever 17D viruViral vectorGenetic stabilitySIV gag IRESSUV GagRecombinant virusFiebre AmarillaCiências BiológicasImproved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragmentinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil / University of Miami. Miller School of Medicine. Department of Pathology. Miami, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.University of Miami. Miller School of Medicine. Department of Pathology. Miami, USA.Fundação Oswaldo Cruz. Instituto deTecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil.We have previously designed a method to construct viable recombinant Yellow Fever (YF) 17D viruses expressing heterologous polypeptides including part of the Simian Immunodeficiency Virus (SIV) Gag protein. However, the expressed region, encompassing amino acid residues from 45 to 269, was genetically unstable. In this study, we improved the genetic stability of this recombinant YF 17D virus by introducing mutations in the IRES element localized at the 5' end of the SIV gag gene. The new stable recombinant virus elicited adaptive immune responses similar to those induced by the original recombinant virus. It is, therefore, possible to increase recombinant stability by removing functional motifs from the insert that may have deleterious effects on recombinant YF viral fitness.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10203/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmarlon_santanetal_IOC_2014.pdfapplication/pdf4845417https://www.arca.fiocruz.br/bitstream/icict/10203/2/marlon_santanetal_IOC_2014.pdf6dd0f945412cceb3fab0ae0abb9e08f7MD52TEXTmarlon_santanetal_IOC_2014.pdf.txtmarlon_santanetal_IOC_2014.pdf.txtExtracted texttext/plain56371https://www.arca.fiocruz.br/bitstream/icict/10203/3/marlon_santanetal_IOC_2014.pdf.txt1e5e8dc2ee8bb04de337383272664414MD53icict/102032022-06-24 13:10:49.057oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:10:49Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment
title Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment
spellingShingle Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment
Santana, Marlon G. Veloso de
Febre Amarela
Yellow Fever 17D viru
Viral vector
Genetic stability
SIV gag IRES
SUV Gag
Recombinant virus
Fiebre Amarilla
Ciências Biológicas
title_short Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment
title_full Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment
title_fullStr Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment
title_full_unstemmed Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment
title_sort Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment
author Santana, Marlon G. Veloso de
author_facet Santana, Marlon G. Veloso de
Neves, Patrícia C. C.
Santos, Juliana Ribeiro dos
Lima, Noemia S.
Santos, Alexandre A. C. dos
Watkins, David I.
Galler, Ricardo
Ronaldo, Myrna C.
author_role author
author2 Neves, Patrícia C. C.
Santos, Juliana Ribeiro dos
Lima, Noemia S.
Santos, Alexandre A. C. dos
Watkins, David I.
Galler, Ricardo
Ronaldo, Myrna C.
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santana, Marlon G. Veloso de
Neves, Patrícia C. C.
Santos, Juliana Ribeiro dos
Lima, Noemia S.
Santos, Alexandre A. C. dos
Watkins, David I.
Galler, Ricardo
Ronaldo, Myrna C.
dc.subject.other.pt_BR.fl_str_mv Febre Amarela
topic Febre Amarela
Yellow Fever 17D viru
Viral vector
Genetic stability
SIV gag IRES
SUV Gag
Recombinant virus
Fiebre Amarilla
Ciências Biológicas
dc.subject.en.pt_BR.fl_str_mv Yellow Fever 17D viru
Viral vector
Genetic stability
SIV gag IRES
SUV Gag
Recombinant virus
dc.subject.es.pt_BR.fl_str_mv Fiebre Amarilla
dc.subject.decs.pt_BR.fl_str_mv Ciências Biológicas
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular de Flavivírus. Rio de Janeiro, RJ, Brasil / University of Miami. Miller School of Medicine. Department of Pathology. Miami, USA.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-05-04T17:07:29Z
dc.date.available.fl_str_mv 2015-05-04T17:07:29Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTANA, Marlon G. Veloso et al. Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment. Virology, n. 452-453, p. 202–211, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10203
dc.identifier.issn.pt_BR.fl_str_mv 1743-422X
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.virol.2014.01.017
identifier_str_mv SANTANA, Marlon G. Veloso et al. Improved genetic stability of recombinant yellow fever 17D virus expressing a lentiviral Gag gene fragment. Virology, n. 452-453, p. 202–211, 2014.
1743-422X
10.1016/j.virol.2014.01.017
url https://www.arca.fiocruz.br/handle/icict/10203
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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