Genomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazil

Detalhes bibliográficos
Autor(a) principal: Anjos, Taís Ramalho dos
Data de Publicação: 2022
Outros Autores: Castro, Vinícius Silva, Machado Filho, Edson Silva, Suffys, Philip Noel, Gomes, Harrison Magdinier, Duarte, Rafael Silva, Figueiredo, Eduardo Eustáquio de Souza, Carvalho, Ricardo César Tavares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/55937
Resumo: Universidade de Cuiabá (UNIC). Faculdade de Medicina Veterinária. Programa de Pós-graduação em Biociência Animal - Stricto Sensu. Cuiabá. MT, Brasil.
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spelling Anjos, Taís Ramalho dosCastro, Vinícius SilvaMachado Filho, Edson SilvaSuffys, Philip NoelGomes, Harrison MagdinierDuarte, Rafael SilvaFigueiredo, Eduardo Eustáquio de SouzaCarvalho, Ricardo César Tavares2022-12-14T17:35:49Z2022-12-14T17:35:49Z2022ANJOS, Taís Ramalho dos et al. Genomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazil. Frontiers in Veterinary Science, v. 9, 1006090, , p. 1 - 14, Nov. 2022.2299-1769https://www.arca.fiocruz.br/handle/icict/5593710.3389/fvets.2022.1006090engFrontiers MediaGenomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade de Cuiabá (UNIC). Faculdade de Medicina Veterinária. Programa de Pós-graduação em Biociência Animal - Stricto Sensu. Cuiabá. MT, Brasil.Universidade Federal de Mato Grosso (UFMT). Faculdade de Agronomia e Zootecnia. Programa de Pós-graduação em Ciência Animal. Cuiabá, MT, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicado a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicado a Micobactérias. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular Aplicado a Micobactérias. Rio de Janeiro, RJ, Brasil.Universidade Federal do Rio de Janeiro (UFRJ). Instituto de Microbiologia Paulo de Góes. Departamento de Microbiologia Médica. Laboratório de Micobactérias. Rio de Janeiro, RJ, Brasil.Universidade Federal de Mato Grosso (UFMT). Faculdade de Nutrição. Laboratório de Microbiologia Molecular de Alimentos. Cuiabá, MT, Brasil.Universidade de Cuiabá (UNIC). Faculdade de Medicina Veterinária. Programa de Pós-graduação em Biociência Animal - Stricto Sensu. Cuiabá. MT, Brasil.The species Mycobacterium tuberculosis variant bovis (M. tuberculosis var. bovis) is associated with tuberculosis, mainly in cattle and bualoes. This pathogen has the potential to infect other mammals, including humans. Tuberculosis caused by M. tuberculosis var. bovis is a zoonosis clinically identical to tuberculosis caused by Mycobacterium tuberculosis, and the recommended treatment in humans results in the use of antibiotics. In this study, we used the whole genome sequencing (WGS) methodology Illumina NovaSeq 6000 System platform to characterize the genome of M. tuberculosis var. bovis in cattle circulating in Mato Grosso, identify mutations related to drug resistance genes, compare with other strains of M. tuberculosis var. bovis brazilian and assess potential drug resistance. Four isolates of M. tuberculosis var. bovis of cattle origin representing the main livestock circuits, which had been more prevalent in previous studies in the state of Mato Grosso, were selected for the genomic study. The genome sizes of the sequenced strains ranged from 4,306,423 to 4,332,964 bp, and the GC content was 65.6%. The four strains from Mato Grosso presented resistance genes to pncA (pyrazinamide), characterized as drug-resistant strains. In addition to verifying several point mutations in the pncA, rpsA, rpsL, gid, rpoB, katG, gyrB, gyrA, tlyA, embA, embB, embC, fgd, fbiB, and fbiC genes, these genes were similar to antibiotic resistance in more than 92% of the Brazilian strains. Therefore, our results indicated a high genetic diversity between our isolates and other M. tuberculosis var. bovis isolated in Brazil. Thus, multiple transmission routes of this pathogen may be present in the production chain. So, to achieve a bovine tuberculosis-free health status, the use of the WGS as a control and monitoring tool will be crucial to determine these transmission routes.Mycobacterium tuberculosis variant bovisMutationPhylogenyDrug resistanceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALEdsonMFilho_PhilipNoel_etal_IOC_2022.pdfEdsonMFilho_PhilipNoel_etal_IOC_2022.pdfapplication/pdf952741https://www.arca.fiocruz.br/bitstream/icict/55937/2/EdsonMFilho_PhilipNoel_etal_IOC_2022.pdf2dac561c3121c16a6d4d54f64f5999adMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/55937/1/license.txt5a560609d32a3863062d77ff32785d58MD51icict/559372023-09-14 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dc.title.en_US.fl_str_mv Genomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazil
title Genomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazil
spellingShingle Genomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazil
Anjos, Taís Ramalho dos
Mycobacterium tuberculosis variant bovis
Mutation
Phylogeny
Drug resistance
title_short Genomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazil
title_full Genomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazil
title_fullStr Genomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazil
title_full_unstemmed Genomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazil
title_sort Genomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazil
author Anjos, Taís Ramalho dos
author_facet Anjos, Taís Ramalho dos
Castro, Vinícius Silva
Machado Filho, Edson Silva
Suffys, Philip Noel
Gomes, Harrison Magdinier
Duarte, Rafael Silva
Figueiredo, Eduardo Eustáquio de Souza
Carvalho, Ricardo César Tavares
author_role author
author2 Castro, Vinícius Silva
Machado Filho, Edson Silva
Suffys, Philip Noel
Gomes, Harrison Magdinier
Duarte, Rafael Silva
Figueiredo, Eduardo Eustáquio de Souza
Carvalho, Ricardo César Tavares
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Anjos, Taís Ramalho dos
Castro, Vinícius Silva
Machado Filho, Edson Silva
Suffys, Philip Noel
Gomes, Harrison Magdinier
Duarte, Rafael Silva
Figueiredo, Eduardo Eustáquio de Souza
Carvalho, Ricardo César Tavares
dc.subject.en.en_US.fl_str_mv Mycobacterium tuberculosis variant bovis
Mutation
Phylogeny
Drug resistance
topic Mycobacterium tuberculosis variant bovis
Mutation
Phylogeny
Drug resistance
description Universidade de Cuiabá (UNIC). Faculdade de Medicina Veterinária. Programa de Pós-graduação em Biociência Animal - Stricto Sensu. Cuiabá. MT, Brasil.
publishDate 2022
dc.date.accessioned.fl_str_mv 2022-12-14T17:35:49Z
dc.date.available.fl_str_mv 2022-12-14T17:35:49Z
dc.date.issued.fl_str_mv 2022
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv ANJOS, Taís Ramalho dos et al. Genomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazil. Frontiers in Veterinary Science, v. 9, 1006090, , p. 1 - 14, Nov. 2022.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/55937
dc.identifier.issn.en_US.fl_str_mv 2299-1769
dc.identifier.doi.none.fl_str_mv 10.3389/fvets.2022.1006090
identifier_str_mv ANJOS, Taís Ramalho dos et al. Genomic analysis of Mycobacterium tuberculosis variant bovis strains isolated from bovine in the state of Mato Grosso, Brazil. Frontiers in Veterinary Science, v. 9, 1006090, , p. 1 - 14, Nov. 2022.
2299-1769
10.3389/fvets.2022.1006090
url https://www.arca.fiocruz.br/handle/icict/55937
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/55937/2/EdsonMFilho_PhilipNoel_etal_IOC_2022.pdf
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