Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas

Detalhes bibliográficos
Autor(a) principal: Gomes, Larissa Rodrigues
Data de Publicação: 2019
Outros Autores: Lavigne, Aline, Brasil, Patrícia, Peterka, Cassio Leonel, Ménard, Didier, Daniel-Ribeiro, Cláudio Tadeu, Ferreira-da-Cruz, Maria de Fátima
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/32100
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.
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spelling Gomes, Larissa RodriguesLavigne, AlineBrasil, PatríciaPeterka, Cassio LeonelMénard, DidierDaniel-Ribeiro, Cláudio TadeuFerreira-da-Cruz, Maria de Fátima2019-03-15T11:52:08Z2019-03-15T11:52:08Z2019GOMES, Larissa Rodrigues et al. Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-6, 2019.0074-0276https://www.arca.fiocruz.br/handle/icict/3210010.1590/0074-027601804251678-8060engInstituto Oswaldo CruzLack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Nacional de Infectologia Evandro Chagas. Laboratório de Doenças Febris Agudas. Rio de Janeiro, RJ, Brasil.Ministério da Saúde. Secretaria de Vigilância em Saúde. Programa Nacional de Prevenção e Controle da Malária. Brasília, DF, Brasil.Institut Pasteur. Biology of Host-Parasite Interactions Unit. Malaria Genetic and Resistance Group. Paris, France.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.Background and objective Brazil is responsible for a large number of Plasmodium vivax cases in America. Given the emergence of P. vivax parasites resistant to chloroquine and the effectiveness of antifolates in vivax malaria treatment together with a correlation between mutations in P. vivax dhfr and dhps genes and SP treatment failure, the point mutations in these genes were investigated. Methods Blood samples from 54 patients experiencing vivax malaria symptomatic episodes in the Amazonian Region were investigated. Genomic DNA was extracted using a DNA extraction kit (QIAGENTM). Nested polymerase chain reaction (PCR) amplification was carried out followed by Sanger sequencing to detect single nucleotide polymorphisms (SNPs). Findings All tested isolates showed non-synonymous mutations in pvdhfr gene: 117N (54/54, 100%) and 58R (25/54, 46%). Double mutant allele 58R/117N (FRTNI, 28%) was the most frequent followed by triple mutant alleles (58R/117N/173L, FRTNL, 11%; 58R/61M/117N, FRMNI, 5% 117N/173L, FSTNL, 4%) and quadruple mutant allele (58R/61M/117N/173L, FRMNL, 2%). A single mutation was observed at codon C383G in pvdhps gene (SGKAV, 48%). Conclusion No evidence of molecular signatures associated with P. vivax resistance to SP was observed in the Brazilian samples.Plasmodium vivaxMalariaPvdhfrPvdhpsChemoresistanceinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83104https://www.arca.fiocruz.br/bitstream/icict/32100/1/license.txt79178e5f2a0eb066867a274556814938MD51ORIGINALve_Gomes_Larissa_etal_INI_2019.pdfve_Gomes_Larissa_etal_INI_2019.pdfapplication/pdf318448https://www.arca.fiocruz.br/bitstream/icict/32100/2/ve_Gomes_Larissa_etal_INI_2019.pdf32042dac37b56b2f43502edac7bd87f8MD52TEXTve_Gomes_Larissa_etal_INI_2019.pdf.txtve_Gomes_Larissa_etal_INI_2019.pdf.txtExtracted texttext/plain26460https://www.arca.fiocruz.br/bitstream/icict/32100/3/ve_Gomes_Larissa_etal_INI_2019.pdf.txt55b705ba5fc0ae0a228df833108a1999MD53icict/321002023-10-30 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dc.title.pt_BR.fl_str_mv Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas
title Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas
spellingShingle Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas
Gomes, Larissa Rodrigues
Plasmodium vivax
Malaria
Pvdhfr
Pvdhps
Chemoresistance
title_short Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas
title_full Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas
title_fullStr Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas
title_full_unstemmed Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas
title_sort Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas
author Gomes, Larissa Rodrigues
author_facet Gomes, Larissa Rodrigues
Lavigne, Aline
Brasil, Patrícia
Peterka, Cassio Leonel
Ménard, Didier
Daniel-Ribeiro, Cláudio Tadeu
Ferreira-da-Cruz, Maria de Fátima
author_role author
author2 Lavigne, Aline
Brasil, Patrícia
Peterka, Cassio Leonel
Ménard, Didier
Daniel-Ribeiro, Cláudio Tadeu
Ferreira-da-Cruz, Maria de Fátima
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Gomes, Larissa Rodrigues
Lavigne, Aline
Brasil, Patrícia
Peterka, Cassio Leonel
Ménard, Didier
Daniel-Ribeiro, Cláudio Tadeu
Ferreira-da-Cruz, Maria de Fátima
dc.subject.en.pt_BR.fl_str_mv Plasmodium vivax
Malaria
Pvdhfr
Pvdhps
Chemoresistance
topic Plasmodium vivax
Malaria
Pvdhfr
Pvdhps
Chemoresistance
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Pesquisa em Malária. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Centro de Pesquisa, Diagnóstico e Treinamento em Malária. Rio de Janeiro, RJ, Brasil.
publishDate 2019
dc.date.accessioned.fl_str_mv 2019-03-15T11:52:08Z
dc.date.available.fl_str_mv 2019-03-15T11:52:08Z
dc.date.issued.fl_str_mv 2019
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv GOMES, Larissa Rodrigues et al. Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-6, 2019.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/32100
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/0074-02760180425
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv GOMES, Larissa Rodrigues et al. Lack of quadruple and quintuple mutant alleles associated with sulfadoxine-pyrimethamine resistance in Plasmodium vivax isolates from Brazilian endemic areas. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 114, p. 1-6, 2019.
0074-0276
10.1590/0074-02760180425
1678-8060
url https://www.arca.fiocruz.br/handle/icict/32100
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz
publisher.none.fl_str_mv Instituto Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/32100/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/32100/2/ve_Gomes_Larissa_etal_INI_2019.pdf
https://www.arca.fiocruz.br/bitstream/icict/32100/3/ve_Gomes_Larissa_etal_INI_2019.pdf.txt
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