Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/14642 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil. |
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Calegar, Deiviane AparecidaNunes, Beatriz CoronatoMonteiro, Kerla Joeline LimaSantos, Jéssica Pereira dosToma, Helena KeikoGomes, Tais FerreiraLima, Marli MariaBóia, Márcio NevesCarvalho-Costa, Filipe Anibal2016-06-23T18:50:16Z2016-07-07T11:59:19Z2016-06-23T18:50:16Z2016-07-07T11:59:19Z2016CALEGAR, Deiviane Aparecida; et al. Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil. Mem Inst Oswaldo Cruz, v.111, n.2, p.114-119, Feb. 2016.0074-0276https://www.arca.fiocruz.br/handle/icict/1464210.1590/0074-027601503831678-8080engFundação Oswaldo CruzParasitosParasitologiaEntamebíaseEpidemiologiaBrasilParasitesProtozoaentamoebiasisEpidemiologyBrazilFrequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escritório Técnico Regional Fiocruz Piauí. Terezina, PI, Brasil.Universidade Federal do Rio de Janeiro. Laboratório de Diagnóstico Molecular e Hematologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ecoepidemiologia da Doença de Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ecoepidemiologia da Doença de Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Escritório Técnico Regional Fiocruz Piauí. Terezina, PI, Brasil.This study aimed to estimate the frequency, associated factors, and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni infections. We performed a survey (n = 213 subjects) to obtain parasitological, sanitation, and sociodemographic data. Faecal samples were processed through flotation and centrifugation methods. E. histolytica, E. dispar, E. moshkovskii, and E. hartmanni were identified by nested-polymerase chain reaction (PCR). The overall prevalence of infection was 22/213 (10.3%). The infection rate among subjects who drink rainwater collected from roofs in tanks was higher than the rate in subjects who drink desalinated water pumped from wells; similarly, the infection rate among subjects who practice open defecation was significantly higher than that of subjects with latrines. Out of the 22 samples positive for morphologically indistinguishable Entamoeba species, the differentiation by PCR was successful for 21. The species distribution was as follows: 57.1% to E. dispar, 23.8% to E. histolytica, 14.3% to E. histolytica and E. dispar, and 4.8% E. dispar and E. hartmanni. These data suggest a high prevalence of asymptomatic infection by the group of morphologically indistinguishable Entamoeba histolytica/dispar/moshkovskii complex and E. hartmanni species. In this context of water scarcity, the sanitary and socioenvironmental characteristics of the region appear to favour transmission.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTdeiviane_calegar_etal_IOC_2016.pdf.txtdeiviane_calegar_etal_IOC_2016.pdf.txtExtracted texttext/plain28002https://www.arca.fiocruz.br/bitstream/icict/14642/4/deiviane_calegar_etal_IOC_2016.pdf.txt0e5f2235fb2e00edf58a08652733d91dMD54ORIGINALdeiviane_calegar_etal_IOC_2016.pdfapplication/pdf619400https://www.arca.fiocruz.br/bitstream/icict/14642/2/deiviane_calegar_etal_IOC_2016.pdfba411f42484bb4ae009503cf495216c5MD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14642/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/146422022-06-24 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dc.title.pt_BR.fl_str_mv |
Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil |
title |
Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil |
spellingShingle |
Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil Calegar, Deiviane Aparecida Parasitos Parasitologia Entamebíase Epidemiologia Brasil Parasites Protozoa entamoebiasis Epidemiology Brazil |
title_short |
Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil |
title_full |
Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil |
title_fullStr |
Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil |
title_full_unstemmed |
Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil |
title_sort |
Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil |
author |
Calegar, Deiviane Aparecida |
author_facet |
Calegar, Deiviane Aparecida Nunes, Beatriz Coronato Monteiro, Kerla Joeline Lima Santos, Jéssica Pereira dos Toma, Helena Keiko Gomes, Tais Ferreira Lima, Marli Maria Bóia, Márcio Neves Carvalho-Costa, Filipe Anibal |
author_role |
author |
author2 |
Nunes, Beatriz Coronato Monteiro, Kerla Joeline Lima Santos, Jéssica Pereira dos Toma, Helena Keiko Gomes, Tais Ferreira Lima, Marli Maria Bóia, Márcio Neves Carvalho-Costa, Filipe Anibal |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Calegar, Deiviane Aparecida Nunes, Beatriz Coronato Monteiro, Kerla Joeline Lima Santos, Jéssica Pereira dos Toma, Helena Keiko Gomes, Tais Ferreira Lima, Marli Maria Bóia, Márcio Neves Carvalho-Costa, Filipe Anibal |
dc.subject.other.pt_BR.fl_str_mv |
Parasitos Parasitologia Entamebíase Epidemiologia Brasil |
topic |
Parasitos Parasitologia Entamebíase Epidemiologia Brasil Parasites Protozoa entamoebiasis Epidemiology Brazil |
dc.subject.en.pt_BR.fl_str_mv |
Parasites Protozoa entamoebiasis Epidemiology Brazil |
description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil. |
publishDate |
2016 |
dc.date.accessioned.fl_str_mv |
2016-06-23T18:50:16Z 2016-07-07T11:59:19Z |
dc.date.available.fl_str_mv |
2016-06-23T18:50:16Z 2016-07-07T11:59:19Z |
dc.date.issued.fl_str_mv |
2016 |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CALEGAR, Deiviane Aparecida; et al. Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil. Mem Inst Oswaldo Cruz, v.111, n.2, p.114-119, Feb. 2016. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/14642 |
dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
dc.identifier.doi.pt_BR.fl_str_mv |
10.1590/0074-02760150383 |
dc.identifier.eissn.pt_BR.fl_str_mv |
1678-8080 |
identifier_str_mv |
CALEGAR, Deiviane Aparecida; et al. Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil. Mem Inst Oswaldo Cruz, v.111, n.2, p.114-119, Feb. 2016. 0074-0276 10.1590/0074-02760150383 1678-8080 |
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https://www.arca.fiocruz.br/handle/icict/14642 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Fundação Oswaldo Cruz |
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Fundação Oswaldo Cruz |
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reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
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