Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil

Detalhes bibliográficos
Autor(a) principal: Calegar, Deiviane Aparecida
Data de Publicação: 2016
Outros Autores: Nunes, Beatriz Coronato, Monteiro, Kerla Joeline Lima, Santos, Jéssica Pereira dos, Toma, Helena Keiko, Gomes, Tais Ferreira, Lima, Marli Maria, Bóia, Márcio Neves, Carvalho-Costa, Filipe Anibal
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14642
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil.
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spelling Calegar, Deiviane AparecidaNunes, Beatriz CoronatoMonteiro, Kerla Joeline LimaSantos, Jéssica Pereira dosToma, Helena KeikoGomes, Tais FerreiraLima, Marli MariaBóia, Márcio NevesCarvalho-Costa, Filipe Anibal2016-06-23T18:50:16Z2016-07-07T11:59:19Z2016-06-23T18:50:16Z2016-07-07T11:59:19Z2016CALEGAR, Deiviane Aparecida; et al. Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil. Mem Inst Oswaldo Cruz, v.111, n.2, p.114-119, Feb. 2016.0074-0276https://www.arca.fiocruz.br/handle/icict/1464210.1590/0074-027601503831678-8080engFundação Oswaldo CruzParasitosParasitologiaEntamebíaseEpidemiologiaBrasilParasitesProtozoaentamoebiasisEpidemiologyBrazilFrequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazilinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escritório Técnico Regional Fiocruz Piauí. Terezina, PI, Brasil.Universidade Federal do Rio de Janeiro. Laboratório de Diagnóstico Molecular e Hematologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ecoepidemiologia da Doença de Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Ecoepidemiologia da Doença de Chagas. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Epidemiologia e Sistemática Molecular. Rio de Janeiro, RJ, Brasil / Fundação Oswaldo Cruz. Escritório Técnico Regional Fiocruz Piauí. Terezina, PI, Brasil.This study aimed to estimate the frequency, associated factors, and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni infections. We performed a survey (n = 213 subjects) to obtain parasitological, sanitation, and sociodemographic data. Faecal samples were processed through flotation and centrifugation methods. E. histolytica, E. dispar, E. moshkovskii, and E. hartmanni were identified by nested-polymerase chain reaction (PCR). The overall prevalence of infection was 22/213 (10.3%). The infection rate among subjects who drink rainwater collected from roofs in tanks was higher than the rate in subjects who drink desalinated water pumped from wells; similarly, the infection rate among subjects who practice open defecation was significantly higher than that of subjects with latrines. Out of the 22 samples positive for morphologically indistinguishable Entamoeba species, the differentiation by PCR was successful for 21. The species distribution was as follows: 57.1% to E. dispar, 23.8% to E. histolytica, 14.3% to E. histolytica and E. dispar, and 4.8% E. dispar and E. hartmanni. These data suggest a high prevalence of asymptomatic infection by the group of morphologically indistinguishable Entamoeba histolytica/dispar/moshkovskii complex and E. hartmanni species. In this context of water scarcity, the sanitary and socioenvironmental characteristics of the region appear to favour transmission.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTdeiviane_calegar_etal_IOC_2016.pdf.txtdeiviane_calegar_etal_IOC_2016.pdf.txtExtracted texttext/plain28002https://www.arca.fiocruz.br/bitstream/icict/14642/4/deiviane_calegar_etal_IOC_2016.pdf.txt0e5f2235fb2e00edf58a08652733d91dMD54ORIGINALdeiviane_calegar_etal_IOC_2016.pdfapplication/pdf619400https://www.arca.fiocruz.br/bitstream/icict/14642/2/deiviane_calegar_etal_IOC_2016.pdfba411f42484bb4ae009503cf495216c5MD52LICENSElicense.txttext/plain2991https://www.arca.fiocruz.br/bitstream/icict/14642/3/license.txt5a560609d32a3863062d77ff32785d58MD53icict/146422022-06-24 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dc.title.pt_BR.fl_str_mv Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil
title Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil
spellingShingle Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil
Calegar, Deiviane Aparecida
Parasitos
Parasitologia
Entamebíase
Epidemiologia
Brasil
Parasites
Protozoa
entamoebiasis
Epidemiology
Brazil
title_short Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil
title_full Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil
title_fullStr Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil
title_full_unstemmed Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil
title_sort Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil
author Calegar, Deiviane Aparecida
author_facet Calegar, Deiviane Aparecida
Nunes, Beatriz Coronato
Monteiro, Kerla Joeline Lima
Santos, Jéssica Pereira dos
Toma, Helena Keiko
Gomes, Tais Ferreira
Lima, Marli Maria
Bóia, Márcio Neves
Carvalho-Costa, Filipe Anibal
author_role author
author2 Nunes, Beatriz Coronato
Monteiro, Kerla Joeline Lima
Santos, Jéssica Pereira dos
Toma, Helena Keiko
Gomes, Tais Ferreira
Lima, Marli Maria
Bóia, Márcio Neves
Carvalho-Costa, Filipe Anibal
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Calegar, Deiviane Aparecida
Nunes, Beatriz Coronato
Monteiro, Kerla Joeline Lima
Santos, Jéssica Pereira dos
Toma, Helena Keiko
Gomes, Tais Ferreira
Lima, Marli Maria
Bóia, Márcio Neves
Carvalho-Costa, Filipe Anibal
dc.subject.other.pt_BR.fl_str_mv Parasitos
Parasitologia
Entamebíase
Epidemiologia
Brasil
topic Parasitos
Parasitologia
Entamebíase
Epidemiologia
Brasil
Parasites
Protozoa
entamoebiasis
Epidemiology
Brazil
dc.subject.en.pt_BR.fl_str_mv Parasites
Protozoa
entamoebiasis
Epidemiology
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia e Parasitologia de Mamíferos Silvestres Reservatórios. Rio de Janeiro, RJ, Brasil.
publishDate 2016
dc.date.accessioned.fl_str_mv 2016-06-23T18:50:16Z
2016-07-07T11:59:19Z
dc.date.available.fl_str_mv 2016-06-23T18:50:16Z
2016-07-07T11:59:19Z
dc.date.issued.fl_str_mv 2016
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv CALEGAR, Deiviane Aparecida; et al. Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil. Mem Inst Oswaldo Cruz, v.111, n.2, p.114-119, Feb. 2016.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14642
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.pt_BR.fl_str_mv 10.1590/0074-02760150383
dc.identifier.eissn.pt_BR.fl_str_mv 1678-8080
identifier_str_mv CALEGAR, Deiviane Aparecida; et al. Frequency and molecular characterisation of Entamoeba histolytica, Entamoeba dispar, Entamoeba moshkovskii, and Entamoeba hartmanni in the context of water scarcity in northeastern Brazil. Mem Inst Oswaldo Cruz, v.111, n.2, p.114-119, Feb. 2016.
0074-0276
10.1590/0074-02760150383
1678-8080
url https://www.arca.fiocruz.br/handle/icict/14642
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
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instacron_str FIOCRUZ
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