Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.

Detalhes bibliográficos
Autor(a) principal: Vale, Ellen de Moura
Data de Publicação: 2014
Outros Autores: Heringer, Angelo Schuabb, Barroso, Tatiana, Ferreira, André Teixeira da Silva, Costa, Monique Nunes da, Perales, Jonas Enrique Aguilar, Catarina, Claudete Santa, Silveira, Vanildo
Tipo de documento: Artigo
Idioma: por
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10706
Resumo: Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB). Laboratório de Biotecnologia. Campos dos Goytacases, RJ, Brasil.
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spelling Vale, Ellen de MouraHeringer, Angelo SchuabbBarroso, TatianaFerreira, André Teixeira da SilvaCosta, Monique Nunes daPerales, Jonas Enrique AguilarCatarina, Claudete SantaSilveira, Vanildo2015-06-08T14:01:54Z2015-06-08T14:01:54Z2014VALE, Ellen de Moura et al. Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.. Proteome Science, v.12:37 , 17p, 2014.1477-5956https://www.arca.fiocruz.br/handle/icict/1070610.1186/1477-5956-12-37porBioMed CentralComparative proteomic analysis of somatic embryo maturation in Carica papaya L.info:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB). Laboratório de Biotecnologia. Campos dos Goytacases, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB). Laboratório de Biotecnologia. Campos dos Goytacases, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB). Laboratório de Biotecnologia. Campos dos Goytacases, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto de Toxinologia. Rio de janeiro, RJ, Brasil.Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto de Toxinologia. Rio de janeiro, RJ, Brasil.Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Instituto de Toxinologia. Rio de janeiro, RJ, Brasil.Rio de Janeiro, RJ, Brasil.Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB).Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB). Laboratório de Biologia Celular e Tecidual. Rio de janeiro, RJ, Brasil.Background Somatic embryogenesis is a complex process regulated by numerous factors. The identification of proteins that are differentially expressed during plant development could result in the development of molecular markers of plant metabolism and provide information contributing to the monitoring and understanding of different biological responses. In addition, the identification of molecular markers could lead to the optimization of protocols allowing the use of biotechnology for papaya propagation and reproduction. This work aimed to investigate the effects of polyethylene glycol (PEG) on somatic embryo development and the protein expression profile during somatic embryo maturation in papaya (Carica papaya L.). Results The maturation treatment supplemented with 6% PEG (PEG6) resulted in the greatest number of somatic embryos and induced differential protein expression compared with cultures grown under the control treatment. Among 135 spots selected for MS/MS analysis, 76 spots were successfully identified, 38 of which were common to both treatments, while 14 spots were unique to the control treatment, and 24 spots were unique to the PEG6 treatment. The identified proteins were assigned to seven categories or were unclassified. The most representative class of proteins observed in the control treatment was associated with the stress response (25.8%), while those under PEG6 treatment were carbohydrate and energy metabolism (18.4%) and the stress response (18.4%). Conclusions The differential expression of three proteins (enolase, esterase and ADH3) induced by PEG6 treatment could play an important role in maturation, and these proteins could be characterized as candidate biomarkers of somatic embryogenesis in papaya.PapayaSomatic embryogenesisProtein profileMass spectrometryTwo-dimensional electrophoresisCarica papaya L.CaricaDesenvolvimento Embrionárioinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10706/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALellen_valletal_IOC_2014.pdfapplication/pdf5367800https://www.arca.fiocruz.br/bitstream/icict/10706/2/ellen_valletal_IOC_2014.pdfdd684f9aec983b8c83cfb6c7430a57c9MD52TEXTellen_valletal_IOC_2014.pdf.txtellen_valletal_IOC_2014.pdf.txtExtracted texttext/plain79052https://www.arca.fiocruz.br/bitstream/icict/10706/3/ellen_valletal_IOC_2014.pdf.txt90b0b5139a2f7fe47bc4dd0b4f950a67MD53icict/107062022-06-24 13:12:33.617oai:www.arca.fiocruz.br:icict/10706TElDRU7Dh0EgREUgRElTVFJJQlVJw4fDg08gTsODTy1FWENMVVNJVkEKCkFvIGNvbmNvcmRhciBlIGFjZWl0YXIgZXN0YSBsaWNlbsOnYSB2b2PDqiAoYXV0b3Igb3UgZGV0ZW50b3IgZG9zIGRpcmVpdG9zIGF1dG9yYWlzKToKCmEpIERlY2xhcmEgcXVlIGNvbmhlY2UgYSBwb2zDrXRpY2EgZGUgY29weXJpZ2h0IGRhIGVkaXRvcmEgZG8gc2V1IGRvY3VtZW50by4KCmIpIERlY2xhcmEgcXVlIGNvbmhlY2UgZSBhY2VpdGEgYXMgRGlyZXRyaXplcyBwYXJhIG8gUmVwb3NpdMOzcmlvIEluc3RpdHVjaW9uYWwgZGEgRnVuZGHDp8OjbyBPc3dhbGRvIENydXogKEZJT0NSVVopLgoKYykgQ29uY2VkZSDDoCBGSU9DUlVaIG8gZGlyZWl0byBuw6NvLWV4Y2x1c2l2byBkZSBhcnF1aXZhciwgcmVwcm9kdXppciwgY29udmVydGVyIChjb21vIGRlZmluaWRvIGEgc2VndWlyKSwgY29tdW5pY2FyCiAKZS9vdSBkaXN0cmlidWlyIG5vIFJlcG9zaXTDs3JpbyBkYSBGSU9DUlVaLCBvIGRvY3VtZW50byBlbnRyZWd1ZSAoaW5jbHVpbmRvIG8gcmVzdW1vL2Fic3RyYWN0KSBlbSBmb3JtYXRvIGRpZ2l0YWwgb3UgCgpwb3IgcXVhbHF1ZXIgb3V0cm8gbWVpby4KCmQpIERlY2xhcmEgcXVlIGF1dG9yaXphIGEgRklPQ1JVWiBhIGFycXVpdmFyIG1haXMgZGUgdW1hIGPDs3BpYSBkZXN0ZSBkb2N1bWVudG8gZSBjb252ZXJ0w6otbG8sIHNlbSBhbHRlcmFyIG8gc2V1IGNvbnRlw7pkbywgCgpwYXJhIHF1YWxxdWVyIGZvcm1hdG8gZGUgYXJxdWl2bywgbWVpbyBvdSBzdXBvcnRlLCBwYXJhIGVmZWl0b3MgZGUgc2VndXJhbsOnYSwgcHJlc2VydmHDp8OjbyAoYmFja3VwKSBlIGFjZXNzby4KCmUpIERlY2xhcmEgcXVlIG8gZG9jdW1lbnRvIHN1Ym1ldGlkbyDDqSBvIHNldSB0cmFiYWxobyBvcmlnaW5hbCwgZSBxdWUgZGV0w6ltIG8gZGlyZWl0byBkZSBjb25jZWRlciBhIHRlcmNlaXJvcyBvcyBkaXJlaXRvcyAKCmNvbnRpZG9zIG5lc3RhIGxpY2Vuw6dhLiBEZWNsYXJhIHRhbWLDqW0gcXVlIGEgZW50cmVnYSBkbyBkb2N1bWVudG8gbsOjbyBpbmZyaW5nZSBvcyBkaXJlaXRvcyBkZSBxdWFscXVlciBvdXRyYSBwZXNzb2Egb3UgZW50aWRhZGUuCgpmKSBEZWNsYXJhIHF1ZSwgbm8gY2FzbyBkbyBkb2N1bWVudG8gc3VibWV0aWRvIGNvbnRlciBtYXRlcmlhbCBkbyBxdWFsIG7Do28gZGV0w6ltIG9zIGRpcmVpdG9zIGRlIGF1dG9yLCBvYnRldmUgYSBhdXRvcml6YcOnw6NvIAoKaXJyZXN0cml0YSBkbyByZXNwZWN0aXZvIGRldGVudG9yIGRlc3NlcyBkaXJlaXRvcywgcGFyYSBjZWRlciBhIEZJT0NSVVogb3MgZGlyZWl0b3MgcmVxdWVyaWRvcyBwb3IgZXN0YSBMaWNlbsOnYSBlIGF1dG9yaXphciBhIAoKdXRpbGl6w6EtbG9zIGxlZ2FsbWVudGUuIERlY2xhcmEgdGFtYsOpbSBxdWUgZXNzZSBtYXRlcmlhbCBjdWpvcyBkaXJlaXRvcyBzw6NvIGRlIHRlcmNlaXJvcyBlc3TDoSBjbGFyYW1lbnRlIGlkZW50aWZpY2FkbyBlIHJlY29uaGVjaWRvIAoKbm8gdGV4dG8gb3UgY29udGXDumRvIGRvIGRvY3VtZW50byBlbnRyZWd1ZS4KCmcpIFNFIE8gRE9DVU1FTlRPIEVOVFJFR1VFIMOJIEJBU0VBRE8gRU0gVFJBQkFMSE8gRklOQU5DSUFETyBPVSBBUE9JQURPIFBPUiBPVVRSQSBJTlNUSVRVScOHw4NPIFFVRSBOw4NPIEEgRklPQ1JVWiwgREVDTEFSQSBRVUUgQ1VNUFJJVSAKClFVQUlTUVVFUiBPQlJJR0HDh8OVRVMgRVhJR0lEQVMgUEVMTyBSRVNQRUNUSVZPIENPTlRSQVRPIE9VIEFDT1JETy4gQSBGSU9DUlVaIGlkZW50aWZpY2Fyw6EgY2xhcmFtZW50ZSBvKHMpIG5vbWUocykgZG8ocykgYXV0b3IoZXMpIGRvcyAKCmRpcmVpdG9zIGRvIGRvY3VtZW50byBlbnRyZWd1ZSBlIG7Do28gZmFyw6EgcXVhbHF1ZXIgYWx0ZXJhw6fDo28sIHBhcmEgYWzDqW0gZG8gcHJldmlzdG8gbmEgYWzDrW5lYSBjKS4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:12:33Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.
title Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.
spellingShingle Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.
Vale, Ellen de Moura
Papaya
Somatic embryogenesis
Protein profile
Mass spectrometry
Two-dimensional electrophoresis
Carica papaya L.
Carica
Desenvolvimento Embrionário
title_short Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.
title_full Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.
title_fullStr Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.
title_full_unstemmed Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.
title_sort Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.
author Vale, Ellen de Moura
author_facet Vale, Ellen de Moura
Heringer, Angelo Schuabb
Barroso, Tatiana
Ferreira, André Teixeira da Silva
Costa, Monique Nunes da
Perales, Jonas Enrique Aguilar
Catarina, Claudete Santa
Silveira, Vanildo
author_role author
author2 Heringer, Angelo Schuabb
Barroso, Tatiana
Ferreira, André Teixeira da Silva
Costa, Monique Nunes da
Perales, Jonas Enrique Aguilar
Catarina, Claudete Santa
Silveira, Vanildo
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Vale, Ellen de Moura
Heringer, Angelo Schuabb
Barroso, Tatiana
Ferreira, André Teixeira da Silva
Costa, Monique Nunes da
Perales, Jonas Enrique Aguilar
Catarina, Claudete Santa
Silveira, Vanildo
dc.subject.en.pt_BR.fl_str_mv Papaya
Somatic embryogenesis
Protein profile
Mass spectrometry
Two-dimensional electrophoresis
Carica papaya L.
topic Papaya
Somatic embryogenesis
Protein profile
Mass spectrometry
Two-dimensional electrophoresis
Carica papaya L.
Carica
Desenvolvimento Embrionário
dc.subject.decs.pt_BR.fl_str_mv Carica
Desenvolvimento Embrionário
description Universidade Estadual do Norte Fluminense Darcy Ribeiro (UENF), Centro de Biociências e Biotecnologia (CBB). Laboratório de Biotecnologia. Campos dos Goytacases, RJ, Brasil.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-08T14:01:54Z
dc.date.available.fl_str_mv 2015-06-08T14:01:54Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv VALE, Ellen de Moura et al. Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.. Proteome Science, v.12:37 , 17p, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10706
dc.identifier.issn.pt_BR.fl_str_mv 1477-5956
dc.identifier.doi.pt_BR.fl_str_mv 10.1186/1477-5956-12-37
identifier_str_mv VALE, Ellen de Moura et al. Comparative proteomic analysis of somatic embryo maturation in Carica papaya L.. Proteome Science, v.12:37 , 17p, 2014.
1477-5956
10.1186/1477-5956-12-37
url https://www.arca.fiocruz.br/handle/icict/10706
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dc.publisher.none.fl_str_mv BioMed Central
publisher.none.fl_str_mv BioMed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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