Anatomy of somatic embryogenesis in Carica papaya L.

Detalhes bibliográficos
Autor(a) principal: Fernando,Juliana A.
Data de Publicação: 2001
Outros Autores: Melo,Murilo, Soares,Marli K. M., Appezzato-da-Glória,Beatriz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Archives of Biology and Technology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300005
Resumo: Mature zygotic embryos of Carica papaya L. ‘Sunrise Solo’ were used as explants for embryogenesis induction. The explants were inoculated on Murashige and Skoog culture medium supplemented with 2 mg.L-1 2,4-dichlorophenoxyacetic acid and incubated in darkness at 25+2°C. Histological analysis of callogenesis and somatic embryogenesis indicated occurrence of direct and indirect somatic embryogenesis development. Direct somatic embryo formation was observed from hypocotyledonary epidermic cells only from explant 18 days after inoculation. Somatic embryos formed indirectly were originated from embryogenic superficial cells of pre-embryonic complexes located on peripherical and on internal cell layers of callus 49 days after inoculation. Diverse morphological differences including disformed embryos were observed among the somatic embryos.
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spelling Anatomy of somatic embryogenesis in Carica papaya L.Anatomysomatic embryogenesispapayaMature zygotic embryos of Carica papaya L. ‘Sunrise Solo’ were used as explants for embryogenesis induction. The explants were inoculated on Murashige and Skoog culture medium supplemented with 2 mg.L-1 2,4-dichlorophenoxyacetic acid and incubated in darkness at 25+2°C. Histological analysis of callogenesis and somatic embryogenesis indicated occurrence of direct and indirect somatic embryogenesis development. Direct somatic embryo formation was observed from hypocotyledonary epidermic cells only from explant 18 days after inoculation. Somatic embryos formed indirectly were originated from embryogenic superficial cells of pre-embryonic complexes located on peripherical and on internal cell layers of callus 49 days after inoculation. Diverse morphological differences including disformed embryos were observed among the somatic embryos.Instituto de Tecnologia do Paraná - Tecpar2001-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300005Brazilian Archives of Biology and Technology v.44 n.3 2001reponame:Brazilian Archives of Biology and Technologyinstname:Instituto de Tecnologia do Paraná (Tecpar)instacron:TECPAR10.1590/S1516-89132001000300005info:eu-repo/semantics/openAccessFernando,Juliana A.Melo,MuriloSoares,Marli K. M.Appezzato-da-Glória,Beatrizeng2002-02-06T00:00:00Zoai:scielo:S1516-89132001000300005Revistahttps://www.scielo.br/j/babt/https://old.scielo.br/oai/scielo-oai.phpbabt@tecpar.br||babt@tecpar.br1678-43241516-8913opendoar:2002-02-06T00:00Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)false
dc.title.none.fl_str_mv Anatomy of somatic embryogenesis in Carica papaya L.
title Anatomy of somatic embryogenesis in Carica papaya L.
spellingShingle Anatomy of somatic embryogenesis in Carica papaya L.
Fernando,Juliana A.
Anatomy
somatic embryogenesis
papaya
title_short Anatomy of somatic embryogenesis in Carica papaya L.
title_full Anatomy of somatic embryogenesis in Carica papaya L.
title_fullStr Anatomy of somatic embryogenesis in Carica papaya L.
title_full_unstemmed Anatomy of somatic embryogenesis in Carica papaya L.
title_sort Anatomy of somatic embryogenesis in Carica papaya L.
author Fernando,Juliana A.
author_facet Fernando,Juliana A.
Melo,Murilo
Soares,Marli K. M.
Appezzato-da-Glória,Beatriz
author_role author
author2 Melo,Murilo
Soares,Marli K. M.
Appezzato-da-Glória,Beatriz
author2_role author
author
author
dc.contributor.author.fl_str_mv Fernando,Juliana A.
Melo,Murilo
Soares,Marli K. M.
Appezzato-da-Glória,Beatriz
dc.subject.por.fl_str_mv Anatomy
somatic embryogenesis
papaya
topic Anatomy
somatic embryogenesis
papaya
description Mature zygotic embryos of Carica papaya L. ‘Sunrise Solo’ were used as explants for embryogenesis induction. The explants were inoculated on Murashige and Skoog culture medium supplemented with 2 mg.L-1 2,4-dichlorophenoxyacetic acid and incubated in darkness at 25+2°C. Histological analysis of callogenesis and somatic embryogenesis indicated occurrence of direct and indirect somatic embryogenesis development. Direct somatic embryo formation was observed from hypocotyledonary epidermic cells only from explant 18 days after inoculation. Somatic embryos formed indirectly were originated from embryogenic superficial cells of pre-embryonic complexes located on peripherical and on internal cell layers of callus 49 days after inoculation. Diverse morphological differences including disformed embryos were observed among the somatic embryos.
publishDate 2001
dc.date.none.fl_str_mv 2001-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300005
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132001000300005
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1516-89132001000300005
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
publisher.none.fl_str_mv Instituto de Tecnologia do Paraná - Tecpar
dc.source.none.fl_str_mv Brazilian Archives of Biology and Technology v.44 n.3 2001
reponame:Brazilian Archives of Biology and Technology
instname:Instituto de Tecnologia do Paraná (Tecpar)
instacron:TECPAR
instname_str Instituto de Tecnologia do Paraná (Tecpar)
instacron_str TECPAR
institution TECPAR
reponame_str Brazilian Archives of Biology and Technology
collection Brazilian Archives of Biology and Technology
repository.name.fl_str_mv Brazilian Archives of Biology and Technology - Instituto de Tecnologia do Paraná (Tecpar)
repository.mail.fl_str_mv babt@tecpar.br||babt@tecpar.br
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