Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2017 |
| Outros Autores: | , , , , |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/20827 https://doi.org/10.1371/journal.pone.0179615 |
Resumo: | Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
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Moreira, Claudia Maria do NascimentoBatista, Cassiano MartinFernandes, Jessica ChimenesKessler, Rafael LuisSoares, Maurilio JoséFragoso, Stenio Perdigão2017-08-29T18:19:54Z2017-08-29T18:19:54Z2017MOREIRA, Claudia Maria do Nascimento et al. Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain. PLoS ONE, v. 12, n. 7, 2017.0179615https://www.arca.fiocruz.br/handle/icict/20827https://doi.org/10.1371/journal.pone.0179615porPublic Library of ScienceTrypanosomaTranscription Factor AP-1ParasitesFactor de Transcripción AP-1ParásitosFator de Transcrição AP-1ParasitosKnockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipaininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil.The AP-1 Adaptor Complex assists clathrin-coated vesicle assembly in the trans-Golgi network (TGN) of eukaryotic cells. However, the role of AP-1 in the protozoan Trypanosoma cruzi-the Chagas disease parasite-has not been addressed. Here, we studied the function and localization of AP-1 in different T. cruzi life cycle forms, by generating a gene knockout of the large AP-1 subunit gamma adaptin (TcAP1-γ), and raising a monoclonal antibody against TcAP1-γ. Co-localization with a Golgi marker and with the clathrin light chain showed that TcAP1-γ is located in the Golgi, and it may interact with clathrin in vivo, at the TGN. Epimastigote (insect form) parasites lacking TcAP1-γ (TcγKO) have reduced proliferation and differentiation into infective metacyclic trypomastigotes (compared with wild-type parasites). TcγKO parasites have also displayed significantly reduced infectivity towards mammalian cells. Importantly, TcAP1-γ knockout impaired maturation and transport to lysosome-related organelles (reservosomes) of a key cargo-the major cysteine protease cruzipain, which is important for parasite nutrition, differentiation and infection. In conclusion, the defective processing and transport of cruzipain upon AP-1 ablation may underlie the phenotype of TcγKO parasites.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83084https://www.arca.fiocruz.br/bitstream/icict/20827/1/license.txt783568c2893d2e25a99990b126be1772MD51ORIGINALMoreiraKnock.pdfMoreiraKnock.pdfapplication/pdf406893https://www.arca.fiocruz.br/bitstream/icict/20827/2/MoreiraKnock.pdf75106aea59834195db3f69aa9be27c85MD52TEXTMoreiraKnock.pdf.txtMoreiraKnock.pdf.txtExtracted texttext/plain75751https://www.arca.fiocruz.br/bitstream/icict/20827/3/MoreiraKnock.pdf.txt365b061d0d59375a8d4f5cf9a8afdff7MD53icict/208272018-08-15 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| dc.title.pt_BR.fl_str_mv |
Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain |
| title |
Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain |
| spellingShingle |
Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain Moreira, Claudia Maria do Nascimento Trypanosoma Transcription Factor AP-1 Parasites Factor de Transcripción AP-1 Parásitos Fator de Transcrição AP-1 Parasitos |
| title_short |
Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain |
| title_full |
Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain |
| title_fullStr |
Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain |
| title_full_unstemmed |
Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain |
| title_sort |
Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain |
| author |
Moreira, Claudia Maria do Nascimento |
| author_facet |
Moreira, Claudia Maria do Nascimento Batista, Cassiano Martin Fernandes, Jessica Chimenes Kessler, Rafael Luis Soares, Maurilio José Fragoso, Stenio Perdigão |
| author_role |
author |
| author2 |
Batista, Cassiano Martin Fernandes, Jessica Chimenes Kessler, Rafael Luis Soares, Maurilio José Fragoso, Stenio Perdigão |
| author2_role |
author author author author author |
| dc.contributor.author.fl_str_mv |
Moreira, Claudia Maria do Nascimento Batista, Cassiano Martin Fernandes, Jessica Chimenes Kessler, Rafael Luis Soares, Maurilio José Fragoso, Stenio Perdigão |
| dc.subject.other.pt_BR.fl_str_mv |
Trypanosoma |
| topic |
Trypanosoma Transcription Factor AP-1 Parasites Factor de Transcripción AP-1 Parásitos Fator de Transcrição AP-1 Parasitos |
| dc.subject.en.pt_BR.fl_str_mv |
Transcription Factor AP-1 Parasites |
| dc.subject.es.pt_BR.fl_str_mv |
Factor de Transcripción AP-1 Parásitos |
| dc.subject.decs.pt_BR.fl_str_mv |
Fator de Transcrição AP-1 Parasitos |
| description |
Fundação Oswaldo Cruz. Instituto Carlos Chagas. Curitiba, PR, Brasil. |
| publishDate |
2017 |
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2017-08-29T18:19:54Z |
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2017-08-29T18:19:54Z |
| dc.date.issued.fl_str_mv |
2017 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
| dc.identifier.citation.fl_str_mv |
MOREIRA, Claudia Maria do Nascimento et al. Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain. PLoS ONE, v. 12, n. 7, 2017. |
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https://www.arca.fiocruz.br/handle/icict/20827 |
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0179615 |
| dc.identifier.doi.none.fl_str_mv |
https://doi.org/10.1371/journal.pone.0179615 |
| identifier_str_mv |
MOREIRA, Claudia Maria do Nascimento et al. Knockout of the gamma subunit of the AP-1 adaptor complex in the human parasite Trypanosoma cruzi impairs infectivity and differentiation and prevents the maturation and targeting of the major protease cruzipain. PLoS ONE, v. 12, n. 7, 2017. 0179615 |
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https://www.arca.fiocruz.br/handle/icict/20827 https://doi.org/10.1371/journal.pone.0179615 |
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por |
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por |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Public Library of Science |
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Public Library of Science |
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