Differential gene expression analysis of sickle cell anemia in steady and crisis state
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2019 |
| Outros Autores: | , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/34731 |
Resumo: | ConselhoNacional deDesenvolvimento Científico eTecnológico,Grant/AwardNumber: 400903/2013-0. |
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Zanette, Dalila LucíolaSantiago, Rayra PereiraLeite, Ivana Paula RibeiroSantana, Sanzio SilvaGuarda, Caroline Conceição daMaffili, Vitor ValérioFerreira, Junia Raquel DutraAdanho, Corynne Stephanie AhouefaYahouedehou, Setondji Cocou Modeste AlexandreMenezes, Isa LyraGoncalves, Marilda Souza2019-08-12T14:08:30Z2019-08-12T14:08:30Z2019ZANETTE, Dalila Lucíola et al. Differential gene expression analysis of sickle cell anemia in steady and crisis state. Annals of Human Genetics, p. 1-8, 2019.0003-4800https://www.arca.fiocruz.br/handle/icict/3473110.1111/ahg.12290ConselhoNacional deDesenvolvimento Científico eTecnológico,Grant/AwardNumber: 400903/2013-0.2020-01-06Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Hospital Pediátrico Professor Hosannah de Oliveira. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Faculdade de Farmacia. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital Pediátrico Professor Hosannah de Oliveira. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil / Universidade Federal da Bahia. Faculdade de Farmacia. Salvador, BA, Brasil.Sickle cell anemia is one of the most prevalent genetic diseases worldwide, showing great clinical heterogeneity. This study compared the gene expression patterns between sickle cell anemia pediatric patients in steady state and in crisis state, as compared to age-paired, healthy individuals. RNA sequencing was performed from these groups of patients/controls using Illumina HiSeq 2500 equipment. The resulting differentially expressed genes were loaded into QIAGEN's ingenuity pathway analysis. The results showed that EIF2 pathway and NRF2-mediated oxidative stress-response pathways were more highly activated both in steady state and in crisis patients, as compared to healthy individuals. In addition, we found increased activation of eIF4 and p70S6K signaling pathways in crisis state compared to healthy individuals. The transcription factor GATA-1 was found exclusively in steady state while SPI was found exclusively in crisis state. IL6 and VEGFA were found only in crisis state, while IL-1B was found exclusively in steady state. The regulator effects analysis revealed IgG1 as an upstream regulator in steady state compared to healthy individuals, resulting in invasion of prostate cancer cell lines as the disease/function outcome. For crisis-state patients versus healthy individuals, two networks of regulator effects revealed STAT1, CD40LG, TGM2, IRF7, IRF4, and IRF1 acting as upstream regulators, resulting in disease/function outcomes, including engulfment of cells and aggregation of blood cells and inflammation of joints. Our results indicated genes and pathways that can provide clues on the molecular events involved in the severity of sickle cell disease.engBlackwell Publishing LtdExpressão gênicaAnemia falciformeVias de sinalizaçãoCrise vaso-oclusivaGene expressionSickle cell anemiaSignaling pathwaysVaso-occlusive crisisDifferential gene expression analysis of sickle cell anemia in steady and crisis stateinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/34731/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALZanette, Dalila L Differential gene.pdfZanette, Dalila L Differential gene.pdfapplication/pdf457560https://www.arca.fiocruz.br/bitstream/icict/34731/2/Zanette%2c%20Dalila%20L%20Differential%20gene.pdf21e2374f4e6fbdf9a9e1f2ee552604d8MD52TEXTZanette, Dalila L Differential gene.pdf.txtZanette, Dalila L Differential gene.pdf.txtExtracted texttext/plain38081https://www.arca.fiocruz.br/bitstream/icict/34731/3/Zanette%2c%20Dalila%20L%20Differential%20gene.pdf.txt3f8a16f071aee52aac21d2800140177eMD53icict/347312023-03-15 14:34:18.989oai:www.arca.fiocruz.br:icict/34731Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório 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| dc.title.pt_BR.fl_str_mv |
Differential gene expression analysis of sickle cell anemia in steady and crisis state |
| title |
Differential gene expression analysis of sickle cell anemia in steady and crisis state |
| spellingShingle |
Differential gene expression analysis of sickle cell anemia in steady and crisis state Zanette, Dalila Lucíola Expressão gênica Anemia falciforme Vias de sinalização Crise vaso-oclusiva Gene expression Sickle cell anemia Signaling pathways Vaso-occlusive crisis |
| title_short |
Differential gene expression analysis of sickle cell anemia in steady and crisis state |
| title_full |
Differential gene expression analysis of sickle cell anemia in steady and crisis state |
| title_fullStr |
Differential gene expression analysis of sickle cell anemia in steady and crisis state |
| title_full_unstemmed |
Differential gene expression analysis of sickle cell anemia in steady and crisis state |
| title_sort |
Differential gene expression analysis of sickle cell anemia in steady and crisis state |
| author |
Zanette, Dalila Lucíola |
| author_facet |
Zanette, Dalila Lucíola Santiago, Rayra Pereira Leite, Ivana Paula Ribeiro Santana, Sanzio Silva Guarda, Caroline Conceição da Maffili, Vitor Valério Ferreira, Junia Raquel Dutra Adanho, Corynne Stephanie Ahouefa Yahouedehou, Setondji Cocou Modeste Alexandre Menezes, Isa Lyra Goncalves, Marilda Souza |
| author_role |
author |
| author2 |
Santiago, Rayra Pereira Leite, Ivana Paula Ribeiro Santana, Sanzio Silva Guarda, Caroline Conceição da Maffili, Vitor Valério Ferreira, Junia Raquel Dutra Adanho, Corynne Stephanie Ahouefa Yahouedehou, Setondji Cocou Modeste Alexandre Menezes, Isa Lyra Goncalves, Marilda Souza |
| author2_role |
author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Zanette, Dalila Lucíola Santiago, Rayra Pereira Leite, Ivana Paula Ribeiro Santana, Sanzio Silva Guarda, Caroline Conceição da Maffili, Vitor Valério Ferreira, Junia Raquel Dutra Adanho, Corynne Stephanie Ahouefa Yahouedehou, Setondji Cocou Modeste Alexandre Menezes, Isa Lyra Goncalves, Marilda Souza |
| dc.subject.other.pt_BR.fl_str_mv |
Expressão gênica Anemia falciforme Vias de sinalização Crise vaso-oclusiva |
| topic |
Expressão gênica Anemia falciforme Vias de sinalização Crise vaso-oclusiva Gene expression Sickle cell anemia Signaling pathways Vaso-occlusive crisis |
| dc.subject.en.pt_BR.fl_str_mv |
Gene expression Sickle cell anemia Signaling pathways Vaso-occlusive crisis |
| description |
ConselhoNacional deDesenvolvimento Científico eTecnológico,Grant/AwardNumber: 400903/2013-0. |
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2019 |
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2019-08-12T14:08:30Z |
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2019-08-12T14:08:30Z |
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2019 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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ZANETTE, Dalila Lucíola et al. Differential gene expression analysis of sickle cell anemia in steady and crisis state. Annals of Human Genetics, p. 1-8, 2019. |
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https://www.arca.fiocruz.br/handle/icict/34731 |
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0003-4800 |
| dc.identifier.doi.none.fl_str_mv |
10.1111/ahg.12290 |
| identifier_str_mv |
ZANETTE, Dalila Lucíola et al. Differential gene expression analysis of sickle cell anemia in steady and crisis state. Annals of Human Genetics, p. 1-8, 2019. 0003-4800 10.1111/ahg.12290 |
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Blackwell Publishing Ltd |
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Blackwell Publishing Ltd |
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