Serodiagnosis of Chronic Chagas Infection by Using EIERecombinant-Chagas-Biomanguinhos Kit
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2001 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/44131 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. |
| id |
CRUZ_5ff2caf672fb17f3dd1009471b07eaa5 |
|---|---|
| oai_identifier_str |
oai:www.arca.fiocruz.br:icict/44131 |
| network_acronym_str |
CRUZ |
| network_name_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| repository_id_str |
2135 |
| spelling |
Gomes, Yara M.Pereira, Valéria R. A.Nakazawa, MineoRosa, Daniela S.Barros, Maria das Neves D. S .Ferreira, Antonio G. P.Silva, Edimilson D.Ogatta, Sueli F. YamadaKrieger, Marco AurélioGoldenberg, Samuel2020-10-22T19:31:54Z2020-10-22T19:31:54Z2001GOMES, Yara M. et al. Serodiagnosis of Chronic Chagas Infection by Using EIE-Recombinant-Chagas-Biomanguinhos Kit . Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 4, p. 497-201, May 2001.0074-0276https://www.arca.fiocruz.br/handle/icict/4413110.1590/S0074-027620010004000091678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.SorodiagnósticoDoença de ChagasTrypanosoma cruziAntígenos recombinantesSerodiagnosisRecombinant antigensTrypanosoma cruziChagas diseaseVacinas SintéticasSerodiagnosis of Chronic Chagas Infection by Using EIERecombinant-Chagas-Biomanguinhos Kitinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil.Universidade Federal de Pernambuco. Hospital Universitário Oswaldo Cruz. Ambulatório de Doença de Chagas. Recife, PE, Brasil..Universidade Federal de Pernambuco. Hospital Universitário Oswaldo Cruz. Ambulatório de Doença de Chagas. Recife, PE, Brasil..Universidade Federal de Pernambuco. Hospital Universitário Oswaldo Cruz. Ambulatório de Doença de Chagas. Recife, PE, Brasil..Universidade Federal de Pernambuco. Hospital Universitário Oswaldo Cruz. Ambulatório de Doença de Chagas. Recife, PE, Brasil..Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Reativos do Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Bio-Manguinhos. Laboratório de Reativos do Instituto de Tecnologia em Imunobiológicos. Rio de Janeiro, RJ, Brasil.Universidade Estadual de Londrina. Departamento de Microbiologia. Londrina, PR, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Entomologia. Bioquímica e Biologia Molecular. Rio de Janeiro, RJ, Brasil.A kit based on an enzyme immunoassay, EIE-Recombinant-Chagas-Biomanguinhos, developed by the Oswaldo Cruz Foundation, was evaluated for the serodiagnosis of chronic Chagas disease. Evaluation was performed with 368 serum samples collected from individuals living in an endemic area for Chagas disease: 131 patients in the chronic phase with confirmed clinical, epidemiological, and serological diagnosis (indirect immunofluorescence, indirect hemagglutination or enzyme-linked immunosorbent assay) and 237 nonchagasic seronegative individuals were considered negative control. The EIE-Recombinant-Chagas-Biomanguinhos kit showed high sensitivity, 100% (CI 95%: 96.4- 100%) and high specificity, 100% (CI 95%: 98-100%). The data obtained were in full agreement with clinical and conventional serology data. In addition, no cross-reaction was observed with sera from patients with cutaneous (n=14) and visceral (n=3) leishmaniasis. However, when these sera were tested by conventional serological assays for Chagas disease, cross-reactions were detected in 14.3% and 33.3% of the patients with cutaneous and visceral leishmaniasis, respectively. No cross-reactions were observed when sera from nonchagasic seronegative patients bearing other infectious disease (syphilis, n=8; HTLV, n=8; HCV, n=7 and HBV, n=12) were tested. In addition, sera of patients with inconclusive results for Chagas disease by conventional serology showed results in agreement with clinical evaluation, when tested by the kit. These results are relevant and indicate that the refered kit provides a safe immunodiagnosis of Chagas disease and could be used in blood bank screening.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44131/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALSamuelGondenberg_YaraMGomes_etal_IOC_2001.pdfSamuelGondenberg_YaraMGomes_etal_IOC_2001.pdfapplication/pdf57220https://www.arca.fiocruz.br/bitstream/icict/44131/2/SamuelGondenberg_YaraMGomes_etal_IOC_2001.pdf6842add1420e8a7d01b2cb60623772d1MD52TEXTSamuelGondenberg_YaraMGomes_etal_IOC_2001.pdf.txtSamuelGondenberg_YaraMGomes_etal_IOC_2001.pdf.txtExtracted texttext/plain21070https://www.arca.fiocruz.br/bitstream/icict/44131/3/SamuelGondenberg_YaraMGomes_etal_IOC_2001.pdf.txt753c7e3ee82880e076787365205b53d7MD53icict/441312022-06-24 13:03:46.576oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:03:46Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Serodiagnosis of Chronic Chagas Infection by Using EIERecombinant-Chagas-Biomanguinhos Kit |
| title |
Serodiagnosis of Chronic Chagas Infection by Using EIERecombinant-Chagas-Biomanguinhos Kit |
| spellingShingle |
Serodiagnosis of Chronic Chagas Infection by Using EIERecombinant-Chagas-Biomanguinhos Kit Gomes, Yara M. Sorodiagnóstico Doença de Chagas Trypanosoma cruzi Antígenos recombinantes Serodiagnosis Recombinant antigens Trypanosoma cruzi Chagas disease Vacinas Sintéticas |
| title_short |
Serodiagnosis of Chronic Chagas Infection by Using EIERecombinant-Chagas-Biomanguinhos Kit |
| title_full |
Serodiagnosis of Chronic Chagas Infection by Using EIERecombinant-Chagas-Biomanguinhos Kit |
| title_fullStr |
Serodiagnosis of Chronic Chagas Infection by Using EIERecombinant-Chagas-Biomanguinhos Kit |
| title_full_unstemmed |
Serodiagnosis of Chronic Chagas Infection by Using EIERecombinant-Chagas-Biomanguinhos Kit |
| title_sort |
Serodiagnosis of Chronic Chagas Infection by Using EIERecombinant-Chagas-Biomanguinhos Kit |
| author |
Gomes, Yara M. |
| author_facet |
Gomes, Yara M. Pereira, Valéria R. A. Nakazawa, Mineo Rosa, Daniela S. Barros, Maria das Neves D. S . Ferreira, Antonio G. P. Silva, Edimilson D. Ogatta, Sueli F. Yamada Krieger, Marco Aurélio Goldenberg, Samuel |
| author_role |
author |
| author2 |
Pereira, Valéria R. A. Nakazawa, Mineo Rosa, Daniela S. Barros, Maria das Neves D. S . Ferreira, Antonio G. P. Silva, Edimilson D. Ogatta, Sueli F. Yamada Krieger, Marco Aurélio Goldenberg, Samuel |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Gomes, Yara M. Pereira, Valéria R. A. Nakazawa, Mineo Rosa, Daniela S. Barros, Maria das Neves D. S . Ferreira, Antonio G. P. Silva, Edimilson D. Ogatta, Sueli F. Yamada Krieger, Marco Aurélio Goldenberg, Samuel |
| dc.subject.other.pt_BR.fl_str_mv |
Sorodiagnóstico Doença de Chagas Trypanosoma cruzi Antígenos recombinantes |
| topic |
Sorodiagnóstico Doença de Chagas Trypanosoma cruzi Antígenos recombinantes Serodiagnosis Recombinant antigens Trypanosoma cruzi Chagas disease Vacinas Sintéticas |
| dc.subject.en.pt_BR.fl_str_mv |
Serodiagnosis Recombinant antigens Trypanosoma cruzi Chagas disease |
| dc.subject.decs.pt_BR.fl_str_mv |
Vacinas Sintéticas |
| description |
Fundação Oswaldo Cruz. Centro de Pesquisas Aggeu Magalhães. Departamento de Imunologia. Recife, PE, Brasil. |
| publishDate |
2001 |
| dc.date.issued.fl_str_mv |
2001 |
| dc.date.accessioned.fl_str_mv |
2020-10-22T19:31:54Z |
| dc.date.available.fl_str_mv |
2020-10-22T19:31:54Z |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.citation.fl_str_mv |
GOMES, Yara M. et al. Serodiagnosis of Chronic Chagas Infection by Using EIE-Recombinant-Chagas-Biomanguinhos Kit . Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 4, p. 497-201, May 2001. |
| dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/44131 |
| dc.identifier.issn.pt_BR.fl_str_mv |
0074-0276 |
| dc.identifier.doi.none.fl_str_mv |
10.1590/S0074-02762001000400009 |
| dc.identifier.eissn.none.fl_str_mv |
1678-8060 |
| identifier_str_mv |
GOMES, Yara M. et al. Serodiagnosis of Chronic Chagas Infection by Using EIE-Recombinant-Chagas-Biomanguinhos Kit . Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 96, n. 4, p. 497-201, May 2001. 0074-0276 10.1590/S0074-02762001000400009 1678-8060 |
| url |
https://www.arca.fiocruz.br/handle/icict/44131 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
| publisher.none.fl_str_mv |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. |
| dc.source.none.fl_str_mv |
reponame:Repositório Institucional da FIOCRUZ (ARCA) instname:Fundação Oswaldo Cruz (FIOCRUZ) instacron:FIOCRUZ |
| instname_str |
Fundação Oswaldo Cruz (FIOCRUZ) |
| instacron_str |
FIOCRUZ |
| institution |
FIOCRUZ |
| reponame_str |
Repositório Institucional da FIOCRUZ (ARCA) |
| collection |
Repositório Institucional da FIOCRUZ (ARCA) |
| bitstream.url.fl_str_mv |
https://www.arca.fiocruz.br/bitstream/icict/44131/1/license.txt https://www.arca.fiocruz.br/bitstream/icict/44131/2/SamuelGondenberg_YaraMGomes_etal_IOC_2001.pdf https://www.arca.fiocruz.br/bitstream/icict/44131/3/SamuelGondenberg_YaraMGomes_etal_IOC_2001.pdf.txt |
| bitstream.checksum.fl_str_mv |
5a560609d32a3863062d77ff32785d58 6842add1420e8a7d01b2cb60623772d1 753c7e3ee82880e076787365205b53d7 |
| bitstream.checksumAlgorithm.fl_str_mv |
MD5 MD5 MD5 |
| repository.name.fl_str_mv |
Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ) |
| repository.mail.fl_str_mv |
repositorio.arca@fiocruz.br |
| _version_ |
1798324793257230336 |