Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2012 |
| Outros Autores: | |
| Tipo de documento: | Artigo |
| Idioma: | por |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/19002 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil. |
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Fonseca, Érica Lourenço daVicente, Ana Carolina Paulo2017-05-23T18:25:46Z2017-05-23T18:25:46Z2012FONSECA, Érica Lourenço da; VICENTE, Ana Carolina Paulo. Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene. Antimicrobial Agents and Chemotherapy, v. 56, n. 6, p. 3392–3394, Jun. 2012.0066-4804https://www.arca.fiocruz.br/handle/icict/19002porGeneqnrVC1 GeneIntegronintrinsic promoterFunctional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Geneinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil.Integrons are natural expression vectors due to the presence of an intrinsic promoter (Pc). Although rare, gene cassettes can harbor their own promoter. This study determined the functionality of an internal promoter in the qnrVC1 cassette whose presence was suggested by a level of transcription similar to that of the preceding cassette (aadA2) and confirmed by in silico analysis. Its functionality was determined by 5= rapid amplification of cDNA ends (RACE) and cloning into promoter-probe vectors. PqnrVC was found in the qnrVC cassette family, stressing its role in contributing to resistance manifestation.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/19002/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALerica_fonseca_anavalente_IOC_2012.pdferica_fonseca_anavalente_IOC_2012.pdfapplication/pdf256443https://www.arca.fiocruz.br/bitstream/icict/19002/2/erica_fonseca_anavalente_IOC_2012.pdf7da15d6964680bae914894ad87cb3ce4MD52TEXTerica_fonseca_anavalente_IOC_2012.pdf.txterica_fonseca_anavalente_IOC_2012.pdf.txtExtracted texttext/plain13874https://www.arca.fiocruz.br/bitstream/icict/19002/3/erica_fonseca_anavalente_IOC_2012.pdf.txt06edf93a0e87eb80e0a1db32a8da4575MD53icict/190022018-08-15 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| dc.title.pt_BR.fl_str_mv |
Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene |
| title |
Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene |
| spellingShingle |
Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene Fonseca, Érica Lourenço da Gene qnrVC1 Gene Integron intrinsic promoter |
| title_short |
Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene |
| title_full |
Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene |
| title_fullStr |
Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene |
| title_full_unstemmed |
Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene |
| title_sort |
Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene |
| author |
Fonseca, Érica Lourenço da |
| author_facet |
Fonseca, Érica Lourenço da Vicente, Ana Carolina Paulo |
| author_role |
author |
| author2 |
Vicente, Ana Carolina Paulo |
| author2_role |
author |
| dc.contributor.author.fl_str_mv |
Fonseca, Érica Lourenço da Vicente, Ana Carolina Paulo |
| dc.subject.other.pt_BR.fl_str_mv |
Gene |
| topic |
Gene qnrVC1 Gene Integron intrinsic promoter |
| dc.subject.en.pt_BR.fl_str_mv |
qnrVC1 Gene |
| dc.subject.en.en.fl_str_mv |
Integron intrinsic promoter |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Genética Molecular de Microrganismos. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2012 |
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2012 |
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2017-05-23T18:25:46Z |
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2017-05-23T18:25:46Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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FONSECA, Érica Lourenço da; VICENTE, Ana Carolina Paulo. Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene. Antimicrobial Agents and Chemotherapy, v. 56, n. 6, p. 3392–3394, Jun. 2012. |
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https://www.arca.fiocruz.br/handle/icict/19002 |
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0066-4804 |
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FONSECA, Érica Lourenço da; VICENTE, Ana Carolina Paulo. Functional Characterization of a Cassette-Specific Promoter in the Class 1 Integron-Associated qnrVC1 Gene. Antimicrobial Agents and Chemotherapy, v. 56, n. 6, p. 3392–3394, Jun. 2012. 0066-4804 |
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