Application of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areas

Detalhes bibliográficos
Autor(a) principal: Rusinol, Marta
Data de Publicação: 2014
Outros Autores: Fernandez-Cassi, Xavier, Hundesa, Ayalkibet, Vieira, Carmen, Kern, Anita, Eriksson, Irene, Ziros, Panos, Kay, David, Miagostovich, Marize, Vargha, Marta, Allard, Annika, Vantarakis, Apostolos, Wyn-Jones, Peter, Bofill-Mas, Silvia, Girones, Rosina
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/10825
Resumo: University of Barcelona. Department of Microbiology.Laboratory of Virus Contaminants of Water and Food. Barcelona, Spain.
id CRUZ_604ad3c1f6d1894255d33191827d62cb
oai_identifier_str oai:www.arca.fiocruz.br:icict/10825
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Rusinol, MartaFernandez-Cassi, XavierHundesa, AyalkibetVieira, CarmenKern, AnitaEriksson, IreneZiros, PanosKay, DavidMiagostovich, MarizeVargha, MartaAllard, AnnikaVantarakis, ApostolosWyn-Jones, PeterBofill-Mas, SilviaGirones, Rosina2015-06-12T13:57:53Z2015-06-12T13:57:53Z2014RUSIÑOL, Marta et al. Application of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areas. Water Research, v.59, p.119-129, 2014.0043-135https://www.arca.fiocruz.br/handle/icict/1082510.1016/j.watres.2014.04.013engElsevierAdenovirusÁgua do RioMicrobial Source Tracking (MST)Água do RioPolyomavirusRiver waterSeawaterPolyomavirusÁgua do MarÁgua do RioApplication of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areasinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversity of Barcelona. Department of Microbiology.Laboratory of Virus Contaminants of Water and Food. Barcelona, Spain.University of Barcelona. Department of Microbiology.Laboratory of Virus Contaminants of Water and Food. Barcelona, Spain.University of Barcelona. Department of Microbiology.Laboratory of Virus Contaminants of Water and Food. Barcelona, Spain.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.National Institute for Environmental Health. Department of Water Microbiology. Budapest, Hungary.Umea° University. Department of Virology. Umeá, Sweden.University of Patras. Medical School. Department of Public Health. Environmental Microbiology Unit. Patras, Greece.Aberystwyth University. Institute of Geography and Earth Sciences (IGES). Durham City, United Kingdom.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Virologia Comparada e Ambiental. Rio de Janeiro, RJ, Brasil.National Institute for Environmental Health. Department of Water Microbiology. Budapest, Hungary.Umea° University. Department of Virology. Umeá, Sweden.University of Patras. Medical School. Department of Public Health. Environmental Microbiology Unit. Patras, Greece.Aberystwyth University. Institute of Geography and Earth Sciences (IGES). Durham City, United Kingdom.University of Barcelona. Department of Microbiology.Laboratory of Virus Contaminants of Water and Food. Barcelona, Spain.University of Barcelona. Department of Microbiology.Laboratory of Virus Contaminants of Water and Food. Barcelona, Spain.Integrated river basin management planning to mitigate the impacts of economic, demographic and climate change is an important issue for the future protection of water resources. Identifying sources of microbial contamination via the emerging science of Microbial Source Tracking (MST) plays a key role in risk assessment and the design of remediation strategies. Following an 18-month surveillance program within the EU-FP7-funded VIROCLIME project, specific MST tools were used to assess human markers such as adenoviruses (HAdV) and JC polyomaviruses (JCPyV) and porcine and bovine markers such as porcine adenoviruses (PAdV) and bovine polyomaviruses (BPyV) via quantification with real-time PCR to analyze surface water collected from five sites within different climatic zones: the Negro River (Brazil), Glafkos River (Greece), Tisza River (Hungary), Llobregat River (Spain) and Umeälven River (Sweden). The utility of the viral MST tools and the prevalence and abundance of specific human and animal viruses in the five river catchments and adjacent seawater, which is impacted by riverine contributions from the upstream catchments, were examined. In areas where no sanitation systems have been implemented, sewage can directly enter surface waters, and river water exhibited high viral loads; HAdV and JCPyV could be detected at mean concentrations of 10(5) and 10(4) Genome Copies/Liter (GC/L), respectively. In general, river water samples upstream of urban discharges presented lower human viral loads than downstream sampling sites, and those differences appeared to increase with urban populations but decrease in response to high river flow, as the elevated river water volume dilutes microbial loads. During dry seasons, river water flow decreases dramatically, and secondary effluents can represent the bulk of the riverine discharge. We also observed that ice cover that formed over the river during the winter in the studied areas in North Europe could preserve viral stability due to the low temperatures and/or the lack of solar inactivation. Porcine and bovine markers were detected where intensive livestock and agricultural activities were present; mean concentration values of 10(3) GC/L indicated that farms were sometimes unexpected and important sources of fecal contamination in water. During spring and summer, when livestock is outdoors and river flows are low, animal pollution increases due to diffuse contamination and direct voiding of feces onto the catchment surface. The field studies described here demonstrate the dynamics of fecal contamination in all catchments studied, and the data obtained is currently being used to develop dissemination models of fecal contamination in water with respect to future climate change scenarios. The results concerning human and animal targets presented in this study demonstrate the specificity and applicability of the viral quantitative parameters developed to widely divergent geographical areas and their high interest as new indicators of human and animal fecal contamination in water and as MST tools.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/10825/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALcarmen_vieiraetal_IOC_2014.pdfapplication/pdf1480469https://www.arca.fiocruz.br/bitstream/icict/10825/2/carmen_vieiraetal_IOC_2014.pdf75c0ef02d512173822cde70b459ec1d5MD52TEXTcarmen_vieiraetal_IOC_2014.pdf.txtcarmen_vieiraetal_IOC_2014.pdf.txtExtracted texttext/plain48240https://www.arca.fiocruz.br/bitstream/icict/10825/3/carmen_vieiraetal_IOC_2014.pdf.txt6e0099bf99c4dde33be69939a79f04bdMD53icict/108252022-06-24 13:10:47.388oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:10:47Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Application of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areas
title Application of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areas
spellingShingle Application of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areas
Rusinol, Marta
Adenovirus
Água do Rio
Microbial Source Tracking (MST)
Água do Rio
Polyomavirus
River water
Seawater
Polyomavirus
Água do Mar
Água do Rio
title_short Application of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areas
title_full Application of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areas
title_fullStr Application of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areas
title_full_unstemmed Application of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areas
title_sort Application of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areas
author Rusinol, Marta
author_facet Rusinol, Marta
Fernandez-Cassi, Xavier
Hundesa, Ayalkibet
Vieira, Carmen
Kern, Anita
Eriksson, Irene
Ziros, Panos
Kay, David
Miagostovich, Marize
Vargha, Marta
Allard, Annika
Vantarakis, Apostolos
Wyn-Jones, Peter
Bofill-Mas, Silvia
Girones, Rosina
author_role author
author2 Fernandez-Cassi, Xavier
Hundesa, Ayalkibet
Vieira, Carmen
Kern, Anita
Eriksson, Irene
Ziros, Panos
Kay, David
Miagostovich, Marize
Vargha, Marta
Allard, Annika
Vantarakis, Apostolos
Wyn-Jones, Peter
Bofill-Mas, Silvia
Girones, Rosina
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Rusinol, Marta
Fernandez-Cassi, Xavier
Hundesa, Ayalkibet
Vieira, Carmen
Kern, Anita
Eriksson, Irene
Ziros, Panos
Kay, David
Miagostovich, Marize
Vargha, Marta
Allard, Annika
Vantarakis, Apostolos
Wyn-Jones, Peter
Bofill-Mas, Silvia
Girones, Rosina
dc.subject.other.pt_BR.fl_str_mv Adenovirus
Água do Rio
topic Adenovirus
Água do Rio
Microbial Source Tracking (MST)
Água do Rio
Polyomavirus
River water
Seawater
Polyomavirus
Água do Mar
Água do Rio
dc.subject.en.pt_BR.fl_str_mv Microbial Source Tracking (MST)
Água do Rio
Polyomavirus
River water
Seawater
dc.subject.decs.pt_BR.fl_str_mv Polyomavirus
Água do Mar
Água do Rio
description University of Barcelona. Department of Microbiology.Laboratory of Virus Contaminants of Water and Food. Barcelona, Spain.
publishDate 2014
dc.date.issued.fl_str_mv 2014
dc.date.accessioned.fl_str_mv 2015-06-12T13:57:53Z
dc.date.available.fl_str_mv 2015-06-12T13:57:53Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv RUSIÑOL, Marta et al. Application of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areas. Water Research, v.59, p.119-129, 2014.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/10825
dc.identifier.issn.pt_BR.fl_str_mv 0043-135
dc.identifier.doi.pt_BR.fl_str_mv 10.1016/j.watres.2014.04.013
identifier_str_mv RUSIÑOL, Marta et al. Application of human and animal viral microbial source tracking tools in fresh and marine waters from five different geographical areas. Water Research, v.59, p.119-129, 2014.
0043-135
10.1016/j.watres.2014.04.013
url https://www.arca.fiocruz.br/handle/icict/10825
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/10825/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/10825/2/carmen_vieiraetal_IOC_2014.pdf
https://www.arca.fiocruz.br/bitstream/icict/10825/3/carmen_vieiraetal_IOC_2014.pdf.txt
bitstream.checksum.fl_str_mv 7d48279ffeed55da8dfe2f8e81f3b81f
75c0ef02d512173822cde70b459ec1d5
6e0099bf99c4dde33be69939a79f04bd
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1813009085591715840

Registros relacionados