Agathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in rats
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2022 |
| Outros Autores: | , , , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/52590 |
Resumo: | Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Coordenação de Pessoal Aperfeiçoamento de Nível Superior (CAPES). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). |
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Nascimento, Ravena P. doJesus, Lívia B. deOliveira Junior, Markley S.Almeida, Aurea M.Moreira, Eduardo L. T.Paredes, Bruno D.David, Jorge M.Souza, Bruno S. F.Costa, Maria De Fátima DiasButt, Arthur M.Silva, Victor Diogenes A.Costa, Silvia L.2022-05-09T17:44:02Z2022-05-09T17:44:02Z2022NASCIMENTO, Ravena P. do et al. Agathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in rats. Frontiers in Pharmacology, v. 13, p. 1-14, 2022.1663-9812https://www.arca.fiocruz.br/handle/icict/5259010.3389/fphar.2022.858190Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Coordenação de Pessoal Aperfeiçoamento de Nível Superior (CAPES). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica. Laboratório de Neuroquímica de Biologia Celular. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica. Laboratório de Neuroquímica de Biologia Celular. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica. Laboratório de Neuroquímica de Biologia Celular. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica. Laboratório de Neuroquímica de Biologia Celular. Salvador, BA, Brasil.Universidade Federal da Bahia. Hospital de Medicina Veterinária. Departamento de Anatomia, Patologia e Clínica Veterinária. Salvador, Brasil.D'Or Instituto de Pesquisa e Educação. Centro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Química. Departamento de Química Geral e Inorgânica. Salvador, BA, Brasil.D'Or Instituto de Pesquisa e Educação. Centro de Biotecnologia e Terapia Celular. Hospital São Rafael. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica. Laboratório de Neuroquímica de Biologia Celular. Salvador, BA, Brasil / INCT– Neurociência Translacional. Salvador, BA, Brasil.University of Portsmouth. School of Pharmacy and Biomedical Sciences. Portsmouth, United Kingdom.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica. Laboratório de Neuroquímica de Biologia Celular. Salvador, BA, Brasil / INCT para Excitotoxicidade e Neuroproteção. Salvador, BA, Brasil.Universidade Federal da Bahia. Instituto de Ciências da Saúde. Departamento de Bioquímica e Biofísica. Laboratório de Neuroquímica de Biologia Celular. Salvador, BA, Brasil / Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil / INCT para Excitotoxicidade e Neuroproteção. Salvador, BA, Brasil.Agathisflavone is a flavonoid with anti-neuroinflammatory and myelinogenic properties, being also capable to induce neurogenesis. This study evaluated the therapeutic effects of agathisflavone—both as a pharmacological therapy administered in vivo and as an in vitro pre-treatment aiming to enhance rat mesenchymal stem cells (r)MSCs properties–in a rat model of acute spinal cord injury (SCI). Adult male Wistar rats (n = 6/group) underwent acute SCI with an F-2 Fogarty catheter and after 4 h were treated daily with agathisflavone (10 mg/kg ip, for 7 days), or administered with a single i.v. dose of 1 × 106 rMSCs either unstimulated cells (control) or pretreated with agathisflavone (1 µM, every 2 days, for 21 days in vitro). Control rats (n = 6/group) were treated with a single dose methylprednisolone (MP, 60 mg/kg ip). BBB scale was used to evaluate the motor functions of the animals; after 7 days of treatment, the SCI area was analyzed after H&E staining, and RT-qPCR was performed to analyze the expression of neurotrophins and arginase. Treatment with agathisflavone alone or with of 21-day agathisflavone–treated rMSCs was able to protect the injured spinal cord tissue, being associated with increased expression of NGF, GDNF and arginase, and reduced macrophage infiltrate. In addition, treatment of animals with agathisflavone alone was able to protect injured spinal cord tissue and to increase expression of neurotrophins, modulating the inflammatory response. These results support a pro-regenerative effect of agathisflavone that holds developmental potential for clinical applications in the future.engFrontiers MediaMSCsCélulas-tronco mesenquimaisAgatisflavonaLesão medular agudaNeurotrofinasRegeneraçãoMSCsMesenchymal stem cellsAgathisflavoneAcute spinal cord injuryNeurotrophinsRegenerationMSCCélulas madre mesenquimalesAgathisflavonaLesión aguda de la médula espinalNeurotrofinasRegeneraciónMSCLes cellules souches mésenchymateusesAgathisflavoneLésion aiguë de la moelle épinièreNeurotrophinesRégénérationCélulas- tronco mesenquimaisFatores de crescimento neuralRegeneraçãoAgathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in ratsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; 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| dc.title.pt_BR.fl_str_mv |
Agathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in rats |
| title |
Agathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in rats |
| spellingShingle |
Agathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in rats Nascimento, Ravena P. do MSCs Células-tronco mesenquimais Agatisflavona Lesão medular aguda Neurotrofinas Regeneração MSCs Mesenchymal stem cells Agathisflavone Acute spinal cord injury Neurotrophins Regeneration MSC Células madre mesenquimales Agathisflavona Lesión aguda de la médula espinal Neurotrofinas Regeneración MSC Les cellules souches mésenchymateuses Agathisflavone Lésion aiguë de la moelle épinière Neurotrophines Régénération Células- tronco mesenquimais Fatores de crescimento neural Regeneração |
| title_short |
Agathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in rats |
| title_full |
Agathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in rats |
| title_fullStr |
Agathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in rats |
| title_full_unstemmed |
Agathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in rats |
| title_sort |
Agathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in rats |
| author |
Nascimento, Ravena P. do |
| author_facet |
Nascimento, Ravena P. do Jesus, Lívia B. de Oliveira Junior, Markley S. Almeida, Aurea M. Moreira, Eduardo L. T. Paredes, Bruno D. David, Jorge M. Souza, Bruno S. F. Costa, Maria De Fátima Dias Butt, Arthur M. Silva, Victor Diogenes A. Costa, Silvia L. |
| author_role |
author |
| author2 |
Jesus, Lívia B. de Oliveira Junior, Markley S. Almeida, Aurea M. Moreira, Eduardo L. T. Paredes, Bruno D. David, Jorge M. Souza, Bruno S. F. Costa, Maria De Fátima Dias Butt, Arthur M. Silva, Victor Diogenes A. Costa, Silvia L. |
| author2_role |
author author author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Nascimento, Ravena P. do Jesus, Lívia B. de Oliveira Junior, Markley S. Almeida, Aurea M. Moreira, Eduardo L. T. Paredes, Bruno D. David, Jorge M. Souza, Bruno S. F. Costa, Maria De Fátima Dias Butt, Arthur M. Silva, Victor Diogenes A. Costa, Silvia L. |
| dc.subject.other.pt_BR.fl_str_mv |
MSCs Células-tronco mesenquimais Agatisflavona Lesão medular aguda Neurotrofinas Regeneração |
| topic |
MSCs Células-tronco mesenquimais Agatisflavona Lesão medular aguda Neurotrofinas Regeneração MSCs Mesenchymal stem cells Agathisflavone Acute spinal cord injury Neurotrophins Regeneration MSC Células madre mesenquimales Agathisflavona Lesión aguda de la médula espinal Neurotrofinas Regeneración MSC Les cellules souches mésenchymateuses Agathisflavone Lésion aiguë de la moelle épinière Neurotrophines Régénération Células- tronco mesenquimais Fatores de crescimento neural Regeneração |
| dc.subject.en.pt_BR.fl_str_mv |
MSCs Mesenchymal stem cells Agathisflavone Acute spinal cord injury Neurotrophins Regeneration |
| dc.subject.es.pt_BR.fl_str_mv |
MSC Células madre mesenquimales Agathisflavona Lesión aguda de la médula espinal Neurotrofinas Regeneración |
| dc.subject.fr.pt_BR.fl_str_mv |
MSC Les cellules souches mésenchymateuses Agathisflavone Lésion aiguë de la moelle épinière Neurotrophines Régénération |
| dc.subject.decs.pt_BR.fl_str_mv |
Células- tronco mesenquimais Fatores de crescimento neural Regeneração |
| description |
Fundação de Amparo à Pesquisa do Estado da Bahia (FAPESB). Coordenação de Pessoal Aperfeiçoamento de Nível Superior (CAPES). Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). |
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2022 |
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2022-05-09T17:44:02Z |
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2022-05-09T17:44:02Z |
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2022 |
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NASCIMENTO, Ravena P. do et al. Agathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in rats. Frontiers in Pharmacology, v. 13, p. 1-14, 2022. |
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https://www.arca.fiocruz.br/handle/icict/52590 |
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1663-9812 |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fphar.2022.858190 |
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NASCIMENTO, Ravena P. do et al. Agathisflavone as a single therapy or in association with mesenchymal stem cells improves tissue repair in a spinal cord injury model in rats. Frontiers in Pharmacology, v. 13, p. 1-14, 2022. 1663-9812 10.3389/fphar.2022.858190 |
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eng |
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Frontiers Media |
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Frontiers Media |
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