Solid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptake

Detalhes bibliográficos
Autor(a) principal: Pires, Vinícius Couto
Data de Publicação: 2020
Outros Autores: Magalhães, Carla Pires, Ferrante, Marcos, Rebouças, Juliana de Souza, Nguewa, Paul, Severino, Patrícia, Barral, Aldina Maria Prado, Veras, Patrícia Sampaio Tavares, Formiga, Fabio Rocha
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/42999
Resumo: Programa de Excelência Acadêmica – Proex (PgPat/UFBA/FIOCRUZ), INCT-MCTI/CNPq/CAPES/FAPs N° 16/2014 and grants from the Fundação de Amparo à Pesquisa do Estado da Bahia – FAPESB and the Conselho Nacional de Pesquisa e Desenvolvimento Científico – CNPq. PSTV holds a grant from CNPq for productivity in research (307832/2015–5). In addition, this study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001. The authors also acknowledge support from the Serviço de Microscopia Eletrônica (SME) – FIOCRUZ/BA for TEM analysis and CIENAM/UFBA for size and zeta potential measurements
id CRUZ_61310deec3fa53f13cbae6ec07a9c2c5
oai_identifier_str oai:www.arca.fiocruz.br:icict/42999
network_acronym_str CRUZ
network_name_str Repositório Institucional da FIOCRUZ (ARCA)
repository_id_str 2135
spelling Pires, Vinícius CoutoMagalhães, Carla PiresFerrante, MarcosRebouças, Juliana de SouzaNguewa, PaulSeverino, PatríciaBarral, Aldina Maria PradoVeras, Patrícia Sampaio TavaresFormiga, Fabio Rocha2020-08-26T16:51:39Z2020-08-26T16:51:39Z2020PIRES, Vinícius Couto et al. Solid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptake. Acta Tropica, p. 1-5, 16 Mar. 2020.0001-706Xhttps://www.arca.fiocruz.br/handle/icict/4299910.1016/j.actatropica.2020.105595Programa de Excelência Acadêmica – Proex (PgPat/UFBA/FIOCRUZ), INCT-MCTI/CNPq/CAPES/FAPs N° 16/2014 and grants from the Fundação de Amparo à Pesquisa do Estado da Bahia – FAPESB and the Conselho Nacional de Pesquisa e Desenvolvimento Científico – CNPq. PSTV holds a grant from CNPq for productivity in research (307832/2015–5). In addition, this study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001. The authors also acknowledge support from the Serviço de Microscopia Eletrônica (SME) – FIOCRUZ/BA for TEM analysis and CIENAM/UFBA for size and zeta potential measurementsFundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade Federal de Lavras. Departamento de Medicina Veterinária. Lavras, MG, Brasil.Universidade de Pernambuco. Instituto de Ciências Biológicas. Recife, PE, Brasil.University of Navarra. Irunlarrea. Instituto de Salud Tropical. Pamplona, Spain.Laboratório de Universidade Tiradentes. Instituto de Tecnologia e Pesquisa. Nanotecnologia e Nanomedicina. Aracaju, SE, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Fundação Oswaldo Cruz. Instituto Gonçalo Moniz. Salvador, BA, Brasil.Universidade de Pernambuco. Programa de Pós-Graduação em Biologia Celular e Molecular Aplicada. Recife, PE, Brasil / Fundação Oswaldo Cruz. Instituto Aggeu Magalhães. Recife, PE, Brasil.17-N-allylamino-17-demethoxygeldanamycin (17-AAG, tanespimycin) is an inhibitor of heat shock protein 90 (Hsp90), which has been studied in the treatment of cancer such as leukemia or solid tumors. Alternatively, 17-AAG may represent a promising therapeutic agent against leishmaniasis. However, the delivery of 17-AAG is difficult due to its poor aqueous solubility. For exploring the therapeutic value of 17-AAG, we developed solid lipid nanoparticles (SLN) by double emulsion method. SLN exhibited ~100 nm, PDI < 0.2 and zeta potential ~20 mV. In addition, SLN were morphologically spherical with negligible aggregation. The entrapment efficiency of 17-AAG into the lipid matrix reached at nearly 80%. In a separate set of experiments, fluorescent SLN (FITC-labeled) showed a remarkable macrophage uptake, peaking within 2 h of incubation by confocal microscopy. Regarding the drug internalization as critical step for elimination of intracellular Leishmania, this finding demonstrates an important feature of the developed SLN. Collectively, these data indicate the feasibility of developing SLN as potential delivery systems for 17-AAG in leishmaniasis chemotherapy.engElsevierNanopartículasLipidiosIn vitroMacrófagosLeishmaniose17-n-allylamino-17-demethoxygeldanamycin (17-aag)Solid lipid nanoparticles (sln)In vitro uptakeMacrophagesLeishmaniasisSolid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptakeinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/42999/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALPires, Vinicius Couto. Solid lipid....pdfPires, Vinicius Couto. Solid lipid....pdfapplication/pdf729560https://www.arca.fiocruz.br/bitstream/icict/42999/2/Pires%2c%20Vinicius%20Couto.%20Solid%20lipid....pdfa7ea377b42504f948588af9fb877ea3eMD52TEXTPires, Vinicius Couto. Solid lipid....pdf.txtPires, Vinicius Couto. Solid lipid....pdf.txtExtracted texttext/plain22416https://www.arca.fiocruz.br/bitstream/icict/42999/3/Pires%2c%20Vinicius%20Couto.%20Solid%20lipid....pdf.txt0a6399f6dc455f9adb1404afa15e9ff7MD53icict/429992023-03-15 14:34:25.5oai:www.arca.fiocruz.br:icict/42999Q0VTU8ODTyBOw4NPIEVYQ0xVU0lWQSBERSBESVJFSVRPUyBBVVRPUkFJUwoKQW8gYWNlaXRhciBvcyBURVJNT1MgZSBDT05EScOHw5VFUyBkZXN0YSBDRVNTw4NPLCBvIEFVVE9SIGUvb3UgVElUVUxBUiBkZSBkaXJlaXRvcwphdXRvcmFpcyBzb2JyZSBhIE9CUkEgZGUgcXVlIHRyYXRhIGVzdGUgZG9jdW1lbnRvOgoKKDEpIENFREUgZSBUUkFOU0ZFUkUsIHRvdGFsIGUgZ3JhdHVpdGFtZW50ZSwgw6AgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaLCBlbQpjYXLDoXRlciBwZXJtYW5lbnRlLCBpcnJldm9nw6F2ZWwgZSBOw4NPIEVYQ0xVU0lWTywgdG9kb3Mgb3MgZGlyZWl0b3MgcGF0cmltb25pYWlzIE7Dg08KQ09NRVJDSUFJUyBkZSB1dGlsaXphw6fDo28gZGEgT0JSQSBhcnTDrXN0aWNhIGUvb3UgY2llbnTDrWZpY2EgaW5kaWNhZGEgYWNpbWEsIGluY2x1c2l2ZSBvcyBkaXJlaXRvcwpkZSB2b3ogZSBpbWFnZW0gdmluY3VsYWRvcyDDoCBPQlJBLCBkdXJhbnRlIHRvZG8gbyBwcmF6byBkZSBkdXJhw6fDo28gZG9zIGRpcmVpdG9zIGF1dG9yYWlzLCBlbQpxdWFscXVlciBpZGlvbWEgZSBlbSB0b2RvcyBvcyBwYcOtc2VzOwoKKDIpIEFDRUlUQSBxdWUgYSBjZXNzw6NvIHRvdGFsIG7Do28gZXhjbHVzaXZhLCBwZXJtYW5lbnRlIGUgaXJyZXZvZ8OhdmVsIGRvcyBkaXJlaXRvcyBhdXRvcmFpcwpwYXRyaW1vbmlhaXMgbsOjbyBjb21lcmNpYWlzIGRlIHV0aWxpemHDp8OjbyBkZSBxdWUgdHJhdGEgZXN0ZSBkb2N1bWVudG8gaW5jbHVpLCBleGVtcGxpZmljYXRpdmFtZW50ZSwKb3MgZGlyZWl0b3MgZGUgZGlzcG9uaWJpbGl6YcOnw6NvIGUgY29tdW5pY2HDp8OjbyBww7pibGljYSBkYSBPQlJBLCBlbSBxdWFscXVlciBtZWlvIG91IHZlw61jdWxvLAppbmNsdXNpdmUgZW0gUmVwb3NpdMOzcmlvcyBEaWdpdGFpcywgYmVtIGNvbW8gb3MgZGlyZWl0b3MgZGUgcmVwcm9kdcOnw6NvLCBleGliacOnw6NvLCBleGVjdcOnw6NvLApkZWNsYW1hw6fDo28sIHJlY2l0YcOnw6NvLCBleHBvc2nDp8OjbywgYXJxdWl2YW1lbnRvLCBpbmNsdXPDo28gZW0gYmFuY28gZGUgZGFkb3MsIHByZXNlcnZhw6fDo28sIGRpZnVzw6NvLApkaXN0cmlidWnDp8OjbywgZGl2dWxnYcOnw6NvLCBlbXByw6lzdGltbywgdHJhZHXDp8OjbywgZHVibGFnZW0sIGxlZ2VuZGFnZW0sIGluY2x1c8OjbyBlbSBub3ZhcyBvYnJhcyBvdQpjb2xldMOibmVhcywgcmV1dGlsaXphw6fDo28sIGVkacOnw6NvLCBwcm9kdcOnw6NvIGRlIG1hdGVyaWFsIGRpZMOhdGljbyBlIGN1cnNvcyBvdSBxdWFscXVlciBmb3JtYSBkZQp1dGlsaXphw6fDo28gbsOjbyBjb21lcmNpYWw7CgooMykgUkVDT05IRUNFIHF1ZSBhIGNlc3PDo28gYXF1aSBlc3BlY2lmaWNhZGEgY29uY2VkZSDDoCBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPCkNSVVogbyBkaXJlaXRvIGRlIGF1dG9yaXphciBxdWFscXVlciBwZXNzb2Eg4oCTIGbDrXNpY2Egb3UganVyw61kaWNhLCBww7pibGljYSBvdSBwcml2YWRhLCBuYWNpb25hbCBvdQplc3RyYW5nZWlyYSDigJMgYSBhY2Vzc2FyIGUgdXRpbGl6YXIgYW1wbGFtZW50ZSBhIE9CUkEsIHNlbSBleGNsdXNpdmlkYWRlLCBwYXJhIHF1YWlzcXVlcgpmaW5hbGlkYWRlcyBuw6NvIGNvbWVyY2lhaXM7CgooNCkgREVDTEFSQSBxdWUgYSBvYnJhIMOpIGNyaWHDp8OjbyBvcmlnaW5hbCBlIHF1ZSDDqSBvIHRpdHVsYXIgZG9zIGRpcmVpdG9zIGFxdWkgY2VkaWRvcyBlIGF1dG9yaXphZG9zLApyZXNwb25zYWJpbGl6YW5kby1zZSBpbnRlZ3JhbG1lbnRlIHBlbG8gY29udGXDumRvIGUgb3V0cm9zIGVsZW1lbnRvcyBxdWUgZmF6ZW0gcGFydGUgZGEgT0JSQSwKaW5jbHVzaXZlIG9zIGRpcmVpdG9zIGRlIHZveiBlIGltYWdlbSB2aW5jdWxhZG9zIMOgIE9CUkEsIG9icmlnYW5kby1zZSBhIGluZGVuaXphciB0ZXJjZWlyb3MgcG9yCmRhbm9zLCBiZW0gY29tbyBpbmRlbml6YXIgZSByZXNzYXJjaXIgYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVogZGUKZXZlbnR1YWlzIGRlc3Blc2FzIHF1ZSB2aWVyZW0gYSBzdXBvcnRhciwgZW0gcmF6w6NvIGRlIHF1YWxxdWVyIG9mZW5zYSBhIGRpcmVpdG9zIGF1dG9yYWlzIG91CmRpcmVpdG9zIGRlIHZveiBvdSBpbWFnZW0sIHByaW5jaXBhbG1lbnRlIG5vIHF1ZSBkaXogcmVzcGVpdG8gYSBwbMOhZ2lvIGUgdmlvbGHDp8O1ZXMgZGUgZGlyZWl0b3M7CgooNSkgQUZJUk1BIHF1ZSBjb25oZWNlIGEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTwpPU1dBTERPIENSVVogZSBhcyBkaXJldHJpemVzIHBhcmEgbyBmdW5jaW9uYW1lbnRvIGRvIHJlcG9zaXTDs3JpbyBpbnN0aXR1Y2lvbmFsIEFSQ0EuCgpBIFBvbMOtdGljYSBJbnN0aXR1Y2lvbmFsIGRlIEFjZXNzbyBBYmVydG8gZGEgRklPQ1JVWiAtIEZVTkRBw4fDg08gT1NXQUxETyBDUlVaIHJlc2VydmEKZXhjbHVzaXZhbWVudGUgYW8gQVVUT1Igb3MgZGlyZWl0b3MgbW9yYWlzIGUgb3MgdXNvcyBjb21lcmNpYWlzIHNvYnJlIGFzIG9icmFzIGRlIHN1YSBhdXRvcmlhCmUvb3UgdGl0dWxhcmlkYWRlLCBzZW5kbyBvcyB0ZXJjZWlyb3MgdXN1w6FyaW9zIHJlc3BvbnPDoXZlaXMgcGVsYSBhdHJpYnVpw6fDo28gZGUgYXV0b3JpYSBlIG1hbnV0ZW7Dp8OjbwpkYSBpbnRlZ3JpZGFkZSBkYSBPQlJBIGVtIHF1YWxxdWVyIHV0aWxpemHDp8Ojby4KCkEgUG9sw610aWNhIEluc3RpdHVjaW9uYWwgZGUgQWNlc3NvIEFiZXJ0byBkYSBGSU9DUlVaIC0gRlVOREHDh8ODTyBPU1dBTERPIENSVVoKcmVzcGVpdGEgb3MgY29udHJhdG9zIGUgYWNvcmRvcyBwcmVleGlzdGVudGVzIGRvcyBBdXRvcmVzIGNvbSB0ZXJjZWlyb3MsIGNhYmVuZG8gYW9zIEF1dG9yZXMKaW5mb3JtYXIgw6AgSW5zdGl0dWnDp8OjbyBhcyBjb25kacOnw7VlcyBlIG91dHJhcyByZXN0cmnDp8O1ZXMgaW1wb3N0YXMgcG9yIGVzdGVzIGluc3RydW1lbnRvcy4KRepositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:34:25Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Solid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptake
title Solid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptake
spellingShingle Solid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptake
Pires, Vinícius Couto
Nanopartículas
Lipidios
In vitro
Macrófagos
Leishmaniose
17-n-allylamino-17-demethoxygeldanamycin (17-aag)
Solid lipid nanoparticles (sln)
In vitro uptake
Macrophages
Leishmaniasis
title_short Solid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptake
title_full Solid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptake
title_fullStr Solid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptake
title_full_unstemmed Solid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptake
title_sort Solid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptake
author Pires, Vinícius Couto
author_facet Pires, Vinícius Couto
Magalhães, Carla Pires
Ferrante, Marcos
Rebouças, Juliana de Souza
Nguewa, Paul
Severino, Patrícia
Barral, Aldina Maria Prado
Veras, Patrícia Sampaio Tavares
Formiga, Fabio Rocha
author_role author
author2 Magalhães, Carla Pires
Ferrante, Marcos
Rebouças, Juliana de Souza
Nguewa, Paul
Severino, Patrícia
Barral, Aldina Maria Prado
Veras, Patrícia Sampaio Tavares
Formiga, Fabio Rocha
author2_role author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Pires, Vinícius Couto
Magalhães, Carla Pires
Ferrante, Marcos
Rebouças, Juliana de Souza
Nguewa, Paul
Severino, Patrícia
Barral, Aldina Maria Prado
Veras, Patrícia Sampaio Tavares
Formiga, Fabio Rocha
dc.subject.other.pt_BR.fl_str_mv Nanopartículas
Lipidios
In vitro
Macrófagos
Leishmaniose
topic Nanopartículas
Lipidios
In vitro
Macrófagos
Leishmaniose
17-n-allylamino-17-demethoxygeldanamycin (17-aag)
Solid lipid nanoparticles (sln)
In vitro uptake
Macrophages
Leishmaniasis
dc.subject.en.pt_BR.fl_str_mv 17-n-allylamino-17-demethoxygeldanamycin (17-aag)
Solid lipid nanoparticles (sln)
In vitro uptake
Macrophages
Leishmaniasis
description Programa de Excelência Acadêmica – Proex (PgPat/UFBA/FIOCRUZ), INCT-MCTI/CNPq/CAPES/FAPs N° 16/2014 and grants from the Fundação de Amparo à Pesquisa do Estado da Bahia – FAPESB and the Conselho Nacional de Pesquisa e Desenvolvimento Científico – CNPq. PSTV holds a grant from CNPq for productivity in research (307832/2015–5). In addition, this study was financed in part by the Coordenação de Aperfeiçoamento de Pessoal de Nível Superior – Brasil (CAPES) – Finance Code 001. The authors also acknowledge support from the Serviço de Microscopia Eletrônica (SME) – FIOCRUZ/BA for TEM analysis and CIENAM/UFBA for size and zeta potential measurements
publishDate 2020
dc.date.accessioned.fl_str_mv 2020-08-26T16:51:39Z
dc.date.available.fl_str_mv 2020-08-26T16:51:39Z
dc.date.issued.fl_str_mv 2020
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv PIRES, Vinícius Couto et al. Solid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptake. Acta Tropica, p. 1-5, 16 Mar. 2020.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/42999
dc.identifier.issn.pt_BR.fl_str_mv 0001-706X
dc.identifier.doi.none.fl_str_mv 10.1016/j.actatropica.2020.105595
identifier_str_mv PIRES, Vinícius Couto et al. Solid lipid nanoparticles as a novel formulation approach for tanespimycin (17-AAG) against leishmania infections: Preparation, characterization and macrophage uptake. Acta Tropica, p. 1-5, 16 Mar. 2020.
0001-706X
10.1016/j.actatropica.2020.105595
url https://www.arca.fiocruz.br/handle/icict/42999
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
bitstream.url.fl_str_mv https://www.arca.fiocruz.br/bitstream/icict/42999/1/license.txt
https://www.arca.fiocruz.br/bitstream/icict/42999/2/Pires%2c%20Vinicius%20Couto.%20Solid%20lipid....pdf
https://www.arca.fiocruz.br/bitstream/icict/42999/3/Pires%2c%20Vinicius%20Couto.%20Solid%20lipid....pdf.txt
bitstream.checksum.fl_str_mv 5a560609d32a3863062d77ff32785d58
a7ea377b42504f948588af9fb877ea3e
0a6399f6dc455f9adb1404afa15e9ff7
bitstream.checksumAlgorithm.fl_str_mv MD5
MD5
MD5
repository.name.fl_str_mv Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)
repository.mail.fl_str_mv repositorio.arca@fiocruz.br
_version_ 1798324743324041216

Registros relacionados