Modulation of Cytokines and Extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant mice
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2020 |
| Outros Autores: | , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/45476 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. |
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Cardoso, Flávia de OliveiraValle, Tânia Zaverucha doSouza, Fernando AlmeidaSilva, Ana Lúcia AbreuCalabrese, Kátia da Silva2020-12-30T14:38:45Z2020-12-30T14:38:45Z2020CARDOSO, Flávia de Oliveira et al. Modulation of cytokines extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant mice. Frontiers in Microbiology, v. 11, Article 1986, p. 1-16, Aug. 2020.1664-302Xhttps://www.arca.fiocruz.br/handle/icict/4547610.3389/fmicb.2020.01986engFrontiers MediaLeishmanioseRatoMatriz extracelularImunopatologiaCitocinasPCR quantitativoReação em Cadeia da Polimerase em Tempo RealLeishmaniasisMiceExtracellular matrixImmunopathologyCytokinesQuantitative PCRReal-time quantitative PCRReação em Cadeia da Polimerase em Tempo RealModulation of Cytokines and Extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant miceinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil / Universidade Estadual do Maranhão. Departamento de Patologia. Laboratório de Anatomopatologia. São Luis, MA, Brasil.Universidade Estadual do Maranhão. Departamento de Patologia. Laboratório de Anatomopatologia. São Luis, MA, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil.Leishmaniases are a complex of diseases with a broad spectrum of clinical forms, which depend on the parasite species, immunological status, and genetic background of the host. In the Leishmania major model, susceptibility is associated with the Th2 pattern of cytokines production, while resistance is associated with Th1 response. However, the same dichotomy does not occur in L. amazonensis-infected mice. Cytokines are key players in these diseases progression, while the extracellular matrix (ECM) components participate in the process of parasite invasion as well as lesion healing. In this article, we analyzed the influence of host genetics on the expression of cytokines, inducible nitric oxide synthase (iNOS), and ECM proteins, as well as the parasite load in mice with different genetic backgrounds infected by L. amazonensis. C57BL/10 and C3H/He mice were subcutaneously infected with 106L. amazonensis promastigotes. Lesion kinetics, parasite load, cytokines, iNOS, and ECM proteins expression were measured by quantitative PCR (qPCR) in the footpad, draining lymph nodes, liver, and spleen at early (24 h and 30 days) and late phase (120 and 180 days) of infection. Analysis of lesion kinetics showed that C57BL/10 mice developed ulcerative lesions at the inoculation site after L. amazonensis infection, while C3H/He showed slight swelling in the footpad 180 days after infection. C57BL/10 showed progressive enhancement of parasite load in all analyzed organs, while C3H/He mice showed extremely low parasite loads. Susceptible C57BL/10 mice showed high levels of TGF-β mRNA in the footpad early in infection and high levels of proinflammatory cytokines mRNA (IL-12, TNF-α, and IFN-γ) and iNOS in the late phase of the infection. There is an association between increased expression of fibronectin, laminin, collagen III and IV, and TGF-β. On the other hand, resistant C3H/He mice presented a lower repertory of cytokines mRNA expression when compared with susceptible C57BL/10 mice, basically producing TNF-α, collagen IV, and laminin early in infection. The findings of our study indicate that L. amazonensis infection induces different cytokine expression in resistant and susceptible mice but not like the L. major model. An organ-compartmentalized cytokine response was observed in our model. Host genetics determine this response, which modulates ECM proteins expression.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/45476/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFlaviaOCardoso_KatiaSCalabrese_etal_IOC_2020.pdfFlaviaOCardoso_KatiaSCalabrese_etal_IOC_2020.pdfapplication/pdf4014078https://www.arca.fiocruz.br/bitstream/icict/45476/2/FlaviaOCardoso_KatiaSCalabrese_etal_IOC_2020.pdfd5541e734b391b179c7e3a4bbfacb737MD52TEXTFlaviaOCardoso_KatiaSCalabrese_etal_IOC_2020.pdf.txtFlaviaOCardoso_KatiaSCalabrese_etal_IOC_2020.pdf.txtExtracted texttext/plain89650https://www.arca.fiocruz.br/bitstream/icict/45476/3/FlaviaOCardoso_KatiaSCalabrese_etal_IOC_2020.pdf.txt4072a158932dcf8e8681b0add0848af5MD53icict/454762022-06-24 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| dc.title.pt_BR.fl_str_mv |
Modulation of Cytokines and Extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant mice |
| title |
Modulation of Cytokines and Extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant mice |
| spellingShingle |
Modulation of Cytokines and Extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant mice Cardoso, Flávia de Oliveira Leishmaniose Rato Matriz extracelular Imunopatologia Citocinas PCR quantitativo Reação em Cadeia da Polimerase em Tempo Real Leishmaniasis Mice Extracellular matrix Immunopathology Cytokines Quantitative PCR Real-time quantitative PCR Reação em Cadeia da Polimerase em Tempo Real |
| title_short |
Modulation of Cytokines and Extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant mice |
| title_full |
Modulation of Cytokines and Extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant mice |
| title_fullStr |
Modulation of Cytokines and Extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant mice |
| title_full_unstemmed |
Modulation of Cytokines and Extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant mice |
| title_sort |
Modulation of Cytokines and Extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant mice |
| author |
Cardoso, Flávia de Oliveira |
| author_facet |
Cardoso, Flávia de Oliveira Valle, Tânia Zaverucha do Souza, Fernando Almeida Silva, Ana Lúcia Abreu Calabrese, Kátia da Silva |
| author_role |
author |
| author2 |
Valle, Tânia Zaverucha do Souza, Fernando Almeida Silva, Ana Lúcia Abreu Calabrese, Kátia da Silva |
| author2_role |
author author author author |
| dc.contributor.author.fl_str_mv |
Cardoso, Flávia de Oliveira Valle, Tânia Zaverucha do Souza, Fernando Almeida Silva, Ana Lúcia Abreu Calabrese, Kátia da Silva |
| dc.subject.other.pt_BR.fl_str_mv |
Leishmaniose Rato Matriz extracelular Imunopatologia Citocinas PCR quantitativo Reação em Cadeia da Polimerase em Tempo Real |
| topic |
Leishmaniose Rato Matriz extracelular Imunopatologia Citocinas PCR quantitativo Reação em Cadeia da Polimerase em Tempo Real Leishmaniasis Mice Extracellular matrix Immunopathology Cytokines Quantitative PCR Real-time quantitative PCR Reação em Cadeia da Polimerase em Tempo Real |
| dc.subject.en.pt_BR.fl_str_mv |
Leishmaniasis Mice Extracellular matrix Immunopathology Cytokines Quantitative PCR Real-time quantitative PCR |
| dc.subject.decs.pt_BR.fl_str_mv |
Reação em Cadeia da Polimerase em Tempo Real |
| description |
Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunomodulação e Protozoologia. Rio de Janeiro, RJ, Brasil. |
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2020 |
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2020-12-30T14:38:45Z |
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2020-12-30T14:38:45Z |
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2020 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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CARDOSO, Flávia de Oliveira et al. Modulation of cytokines extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant mice. Frontiers in Microbiology, v. 11, Article 1986, p. 1-16, Aug. 2020. |
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https://www.arca.fiocruz.br/handle/icict/45476 |
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1664-302X |
| dc.identifier.doi.none.fl_str_mv |
10.3389/fmicb.2020.01986 |
| identifier_str_mv |
CARDOSO, Flávia de Oliveira et al. Modulation of cytokines extracellular matrix proteins expression by Leishmania amazonensis in susceptible and resistant mice. Frontiers in Microbiology, v. 11, Article 1986, p. 1-16, Aug. 2020. 1664-302X 10.3389/fmicb.2020.01986 |
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Frontiers Media |
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