Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR

Detalhes bibliográficos
Autor(a) principal: D'Ávila, Daniella Alchaar
Data de Publicação: 2018
Outros Autores: Galvão, Lúcia Maria C., Sousa, Giovane R., Britto, Constança, Moreira, Otacilio C., Chiari, Egler
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/31226
Resumo: Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Programa de Pós-Graduação em Parasitologia. Belo Horizonte, MG, Brasil.
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spelling D'Ávila, Daniella AlchaarGalvão, Lúcia Maria C.Sousa, Giovane R.Britto, ConstançaMoreira, Otacilio C.Chiari, Egler2019-01-22T15:25:08Z2019-01-22T15:25:08Z2018D`AVILA, Daniella Alchaar; et al. Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR. Plos One, v.13, n.11, e0208133, 16p, Nov. 2018.1932-6203https://www.arca.fiocruz.br/handle/icict/3122610.1371/journal.pone.0208133engPublic Library of ScienceDoença de ChagasCarga parasitáriaHemoculturaReação em Cadeia da Polimerase em Tempo realPacientesChagas DiseaseParasite loadBlood cultureQuantitative real time PCRPatientsReação em Cadeia da Polimerase em Tempo RealMonitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCRinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleUniversidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Programa de Pós-Graduação em Parasitologia. Belo Horizonte, MG, Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Programa de Pós-Graduação em Parasitologia. Belo Horizonte, MG, Brasil.Harvard Medical School. Joslin Diabetes Center. Section on Immunobiology. Boston, MA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Biologia Molecular e Doenças Endêmicas. Rio de Janeiro, RJ. Brasil.Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Programa de Pós-Graduação em Parasitologia. Belo Horizonte, MG, Brasil.Despite the improvements in diagnostic tools for detection of Trypanosoma cruzi in human blood samples, the isolation of parasite from bloodstream in the chronic phase of Chagas disease is challenging. Thus, there is an increasing interest in the development of strategies that allow an accurate monitoring of the parasite load in bloodstream of Chagas disease patients. Given that, the comparison of a classical diagnostic method such as blood culture and multiplex quantitative real-time PCR (qPCR) was few explored so far. Therefore, this study aimed to compare the detection and quantification of T. cruzi load in the circulating blood of patients with chronic Chagas disease, using blood culture and qPCR techniques.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/31226/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALoctaciliomoreira_constancabrito_etal_IOC_2018.pdfoctaciliomoreira_constancabrito_etal_IOC_2018.pdfapplication/pdf1085095https://www.arca.fiocruz.br/bitstream/icict/31226/2/octaciliomoreira_constancabrito_etal_IOC_2018.pdfde53150ee3d08baae6fad1386a17fa17MD52TEXToctaciliomoreira_constancabrito_etal_IOC_2018.pdf.txtoctaciliomoreira_constancabrito_etal_IOC_2018.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR
title Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR
spellingShingle Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR
D'Ávila, Daniella Alchaar
Doença de Chagas
Carga parasitária
Hemocultura
Reação em Cadeia da Polimerase em Tempo real
Pacientes
Chagas Disease
Parasite load
Blood culture
Quantitative real time PCR
Patients
Reação em Cadeia da Polimerase em Tempo Real
title_short Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR
title_full Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR
title_fullStr Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR
title_full_unstemmed Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR
title_sort Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR
author D'Ávila, Daniella Alchaar
author_facet D'Ávila, Daniella Alchaar
Galvão, Lúcia Maria C.
Sousa, Giovane R.
Britto, Constança
Moreira, Otacilio C.
Chiari, Egler
author_role author
author2 Galvão, Lúcia Maria C.
Sousa, Giovane R.
Britto, Constança
Moreira, Otacilio C.
Chiari, Egler
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv D'Ávila, Daniella Alchaar
Galvão, Lúcia Maria C.
Sousa, Giovane R.
Britto, Constança
Moreira, Otacilio C.
Chiari, Egler
dc.subject.other.pt_BR.fl_str_mv Doença de Chagas
Carga parasitária
Hemocultura
Reação em Cadeia da Polimerase em Tempo real
Pacientes
topic Doença de Chagas
Carga parasitária
Hemocultura
Reação em Cadeia da Polimerase em Tempo real
Pacientes
Chagas Disease
Parasite load
Blood culture
Quantitative real time PCR
Patients
Reação em Cadeia da Polimerase em Tempo Real
dc.subject.en.pt_BR.fl_str_mv Chagas Disease
Parasite load
Blood culture
Quantitative real time PCR
Patients
dc.subject.decs.pt_BR.fl_str_mv Reação em Cadeia da Polimerase em Tempo Real
description Universidade Federal de Minas Gerais. Instituto de Ciências Biológicas. Departamento de Parasitologia. Programa de Pós-Graduação em Parasitologia. Belo Horizonte, MG, Brasil.
publishDate 2018
dc.date.issued.fl_str_mv 2018
dc.date.accessioned.fl_str_mv 2019-01-22T15:25:08Z
dc.date.available.fl_str_mv 2019-01-22T15:25:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv D`AVILA, Daniella Alchaar; et al. Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR. Plos One, v.13, n.11, e0208133, 16p, Nov. 2018.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/31226
dc.identifier.issn.pt_BR.fl_str_mv 1932-6203
dc.identifier.doi.none.fl_str_mv 10.1371/journal.pone.0208133
identifier_str_mv D`AVILA, Daniella Alchaar; et al. Monitoring the parasite load in chronic Chagas disease patients: comparison between blood culture and quantitative real time PCR. Plos One, v.13, n.11, e0208133, 16p, Nov. 2018.
1932-6203
10.1371/journal.pone.0208133
url https://www.arca.fiocruz.br/handle/icict/31226
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Public Library of Science
publisher.none.fl_str_mv Public Library of Science
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
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instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
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collection Repositório Institucional da FIOCRUZ (ARCA)
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