Localization of fibroblast growth factor 23 protein in the rat hypothalamus
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2021 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/48479 |
Resumo: | Endocrine Laboratory, Department of Clinical Chemistry, Amsterdam Gastroenterology & Metabolism, Amsterdam UMC, Vrije Universiteit Amsterdam and University of Amsterdam.Amsterdam, The Netherlands. |
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Ursem, Stan R.Diepenbroek, CharleneBacic, VesnaUnmehopa, Unga A.Eggels, LeslieMonteiro, Clarissa M. MayaHeijboer, Annemieke C.la Fleur, Susanne E.2021-08-02T20:17:58Z2021-08-02T20:17:58Z2021URSEM, Stan R. et al. Localization of fibroblast growth factor 23 protein in the rat hypothalamus. European Journal of Neuroscience, v. 1, n. 11, 11 p, 2021.0953-816Xhttps://www.arca.fiocruz.br/handle/icict/4847910.1111/ejn.15375engWileyHipotálamoFator de crescimento do fibroblasto 23Terceiro ventrículoKlothoFGF23FGF23Fibroblast growth factor 23HypothalamusKlothoThird ventricleTerceiro VentrículoLocalization of fibroblast growth factor 23 protein in the rat hypothalamusinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleEndocrine Laboratory, Department of Clinical Chemistry, Amsterdam Gastroenterology & Metabolism, Amsterdam UMC, Vrije Universiteit Amsterdam and University of Amsterdam.Amsterdam, The Netherlands.Department of Endocrinology and Metabolism and Laboratory of Endocrinology, Department of Clinical Chemistry, Amsterdam Neuroscience, Amsterdam UMC, University of Amsterdam. Amsterdam, The Netherlands / Metabolism and Reward Group, Netherlands Institute for Neuroscience, An Institute of the Royal Netherlands Academy of Arts and Sciences (KNAW). Amsterdam, The Netherlands.Department of Endocrinology and Metabolism and Laboratory of Endocrinology, Department of Clinical Chemistry, Amsterdam Neuroscience, Amsterdam UMC, University of Amsterdam. Amsterdam, The Netherlands / Metabolism and Reward Group, Netherlands Institute for Neuroscience, An Institute of the Royal Netherlands Academy of Arts and Sciences (KNAW). Amsterdam, The Netherlands.Department of Endocrinology and Metabolism and Laboratory of Endocrinology, Department of Clinical Chemistry, Amsterdam Neuroscience, Amsterdam UMC, University of Amsterdam. Amsterdam, The Netherlands / Metabolism and Reward Group, Netherlands Institute for Neuroscience, An Institute of the Royal Netherlands Academy of Arts and Sciences (KNAW). Amsterdam, The Netherlands.Department of Endocrinology and Metabolism and Laboratory of Endocrinology, Department of Clinical Chemistry, Amsterdam Neuroscience, Amsterdam UMC, University of Amsterdam. Amsterdam, The Netherlands / Metabolism and Reward Group, Netherlands Institute for Neuroscience, An Institute of the Royal Netherlands Academy of Arts and Sciences (KNAW). Amsterdam, The Netherlands.Department of Endocrinology and Metabolism and Laboratory of Endocrinology, Department of Clinical Chemistry, Amsterdam Neuroscience, Amsterdam UMC, University of Amsterdam. Amsterdam, The Netherlands / Metabolism and Reward Group, Netherlands Institute for Neuroscience, An Institute of the Royal Netherlands Academy of Arts and Sciences (KNAW). Amsterdam, The Netherlands / Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunofarmacologia. Rio de Janeiro, RJ, Brasil.Endocrine Laboratory, Department of Clinical Chemistry, Amsterdam Gastroenterology & Metabolism, Amsterdam UMC, Vrije Universiteit Amsterdam and University of Amsterdam.Amsterdam, The Netherlands.Department of Endocrinology and Metabolism and Laboratory of Endocrinology, Department of Clinical Chemistry, Amsterdam Neuroscience, Amsterdam UMC, University of Amsterdam. Amsterdam, The Netherlands / Metabolism and Reward Group, Netherlands Institute for Neuroscience, An Institute of the Royal Netherlands Academy of Arts and Sciences (KNAW). Amsterdam, The Netherlands.Fibroblast growth factor 23 (FGF23) is an endocrine growth factor and known to play a pivotal role in phosphate homeostasis. Interestingly, several studies point towards a function of FGF23 in the hypothalamus. FGF23 clas sically activates the FGF receptor 1 in the presence of the co-receptor αKlotho, of both gene expression in the brain was previously established. However, studies on gene and protein expression of FGF23 in the brain are scarce and have been inconsistent. Therefore, our aim was to localise FGF23 gene and protein expression in the rat brain with focus on the hypothalamus. Also, we investigated the protein expression of αKlotho. Adult rat brains were used to localise and visualise FGF23 and αKlotho protein in the hypo thalamus by immunofluorescence labelling. Furthermore, western blots were used for assessing hypothalamic FGF23 protein expression. FGF23 gene expression was investigated by qPCR in punches of the arcuate nucleus, lat eral hypothalamus, paraventricular nucleus, choroid plexus, ventrolateral thalamic nucleus and the ventromedial hypothalamus. Immunoreactivity for FGF23 and αKlotho protein was found in the hypothalamus, third ventricle lining and the choroid plexus. Western blot analysis of the hypothalamus Fibroblast growth factor 23 (FGF23) is an endocrine growth factor and known to play a pivotal role in phosphate homeostasis. Interestingly, several studies point towards a function of FGF23 in the hypothalamus. FGF23 clas sically activates the FGF receptor 1 in the presence of the co-receptor αKlotho, of both gene expression in the brain was previously established. However, studies on gene and protein expression of FGF23 in the brain are scarce and have been inconsistent. Therefore, our aim was to localise FGF23 gene and protein expression in the rat brain with focus on the hypothalamus. Also, we investigated the protein expression of αKlotho. Adult rat brains were used to localise and visualise FGF23 and αKlotho protein in the hypo thalamus by immunofluorescence labelling. Furthermore, western blots were used for assessing hypothalamic FGF23 protein expression. FGF23 gene expression was investigated by qPCR in punches of the arcuate nucleus, lat eral hypothalamus, paraventricular nucleus, choroid plexus, ventrolateral thalamic nucleus and the ventromedial hypothalamus. Immunoreactivity for FGF23 and αKlotho protein was found in the hypothalamus, third ventricle lining and the choroid plexus. Western blot analysis of the hypothalamusFibroblast growth factor 23 (FGF23) is an endocrine growth factor and known to play a pivotal role in phosphate homeostasis. Interestingly, several studies point towards a function of FGF23 in the hypothalamus. FGF23 clas sically activates the FGF receptor 1 in the presence of the co-receptor αKlotho, of both gene expression in the brain was previously established. However, studies on gene and protein expression of FGF23 in the brain are scarce and have been inconsistent. Therefore, our aim was to localise FGF23 gene and protein expression in the rat brain with focus on the hypothalamus. Also, we investigated the protein expression of αKlotho. Adult rat brains were used to localise and visualise FGF23 and αKlotho protein in the hypo thalamus by immunofluorescence labelling. Furthermore, western blots were used for assessing hypothalamic FGF23 protein expression. FGF23 gene expression was investigated by qPCR in punches of the arcuate nucleus, lat eral hypothalamus, paraventricular nucleus, choroid plexus, ventrolateral thalamic nucleus and the ventromedial hypothalamus. Immunoreactivity for FGF23 and αKlotho protein was found in the hypothalamus, third ventricle lining and the choroid plexus. Western blot analysis of the hypothalamusconfirmed the presence of FGF23. Gene expression of FGF23 was not detected, suggesting that the observed FGF23 protein is not brain-derived. Several FGF receptors are known to be present in the brain. Therefore, we conclude that the machinery for FGF23 signal transduction is present in sev eral brain areas, indeed suggesting a role for FGF23 in the brain.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/48479/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALClarissaM_Monteiro_etal_IOC_2021.pdfClarissaM_Monteiro_etal_IOC_2021.pdfapplication/pdf5850412https://www.arca.fiocruz.br/bitstream/icict/48479/2/ClarissaM_Monteiro_etal_IOC_2021.pdf0115f4cc130a1c6f90829e5ea2670de0MD52TEXTClarissaM_Monteiro_etal_IOC_2021.pdf.txtClarissaM_Monteiro_etal_IOC_2021.pdf.txtExtracted texttext/plain43077https://www.arca.fiocruz.br/bitstream/icict/48479/3/ClarissaM_Monteiro_etal_IOC_2021.pdf.txtdf135fab858bc8610e8116253a59ee29MD53icict/484792021-08-03 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| dc.title.pt_BR.fl_str_mv |
Localization of fibroblast growth factor 23 protein in the rat hypothalamus |
| title |
Localization of fibroblast growth factor 23 protein in the rat hypothalamus |
| spellingShingle |
Localization of fibroblast growth factor 23 protein in the rat hypothalamus Ursem, Stan R. Hipotálamo Fator de crescimento do fibroblasto 23 Terceiro ventrículo Klotho FGF23 FGF23 Fibroblast growth factor 23 Hypothalamus Klotho Third ventricle Terceiro Ventrículo |
| title_short |
Localization of fibroblast growth factor 23 protein in the rat hypothalamus |
| title_full |
Localization of fibroblast growth factor 23 protein in the rat hypothalamus |
| title_fullStr |
Localization of fibroblast growth factor 23 protein in the rat hypothalamus |
| title_full_unstemmed |
Localization of fibroblast growth factor 23 protein in the rat hypothalamus |
| title_sort |
Localization of fibroblast growth factor 23 protein in the rat hypothalamus |
| author |
Ursem, Stan R. |
| author_facet |
Ursem, Stan R. Diepenbroek, Charlene Bacic, Vesna Unmehopa, Unga A. Eggels, Leslie Monteiro, Clarissa M. Maya Heijboer, Annemieke C. la Fleur, Susanne E. |
| author_role |
author |
| author2 |
Diepenbroek, Charlene Bacic, Vesna Unmehopa, Unga A. Eggels, Leslie Monteiro, Clarissa M. Maya Heijboer, Annemieke C. la Fleur, Susanne E. |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Ursem, Stan R. Diepenbroek, Charlene Bacic, Vesna Unmehopa, Unga A. Eggels, Leslie Monteiro, Clarissa M. Maya Heijboer, Annemieke C. la Fleur, Susanne E. |
| dc.subject.other.pt_BR.fl_str_mv |
Hipotálamo Fator de crescimento do fibroblasto 23 Terceiro ventrículo Klotho FGF23 |
| topic |
Hipotálamo Fator de crescimento do fibroblasto 23 Terceiro ventrículo Klotho FGF23 FGF23 Fibroblast growth factor 23 Hypothalamus Klotho Third ventricle Terceiro Ventrículo |
| dc.subject.en.pt_BR.fl_str_mv |
FGF23 Fibroblast growth factor 23 Hypothalamus Klotho Third ventricle |
| dc.subject.decs.pt_BR.fl_str_mv |
Terceiro Ventrículo |
| description |
Endocrine Laboratory, Department of Clinical Chemistry, Amsterdam Gastroenterology & Metabolism, Amsterdam UMC, Vrije Universiteit Amsterdam and University of Amsterdam.Amsterdam, The Netherlands. |
| publishDate |
2021 |
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2021-08-02T20:17:58Z |
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2021-08-02T20:17:58Z |
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2021 |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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URSEM, Stan R. et al. Localization of fibroblast growth factor 23 protein in the rat hypothalamus. European Journal of Neuroscience, v. 1, n. 11, 11 p, 2021. |
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https://www.arca.fiocruz.br/handle/icict/48479 |
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0953-816X |
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10.1111/ejn.15375 |
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URSEM, Stan R. et al. Localization of fibroblast growth factor 23 protein in the rat hypothalamus. European Journal of Neuroscience, v. 1, n. 11, 11 p, 2021. 0953-816X 10.1111/ejn.15375 |
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https://www.arca.fiocruz.br/handle/icict/48479 |
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Wiley |
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Wiley |
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