Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature

Detalhes bibliográficos
Autor(a) principal: Costa, Gabriel Luíz
Data de Publicação: 2017
Outros Autores: Amaral, Lara Cotta, Fontes, Cor Jesus Fernandes, Carvalho, Luzia Helena de, Brito, Cristiana Ferreira Alves de, Sousa, Taís Nóbrega de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/25477
Resumo: 2020-01-01
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spelling Costa, Gabriel LuízAmaral, Lara CottaFontes, Cor Jesus FernandesCarvalho, Luzia Helena deBrito, Cristiana Ferreira Alves deSousa, Taís Nóbrega de2018-03-23T18:13:08Z2018-03-23T18:13:08Z2017COSTA, Gabriel Luíz et al. Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature. Malaria Journal, v. 16, art. 152, 20171475-2875https://www.arca.fiocruz.br/handle/icict/2547710.1186/s12936-017-1806-zengBiomed CentralMalariaPlasmodium falciparumPlasmodium vivaxresistencia a drogasMalariaPlasmodium falciparumPlasmodium vivaxDrug resistanceAssessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literatureinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/article2020-01-01Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Biologia Molecular e Grupo de Pesquisa Imunologia em Malária. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Biologia Molecular e Grupo de Pesquisa Imunologia em Malária. Belo Horizonte, MG, BrasilUniversidade Federal de Mato Grosso. Hospital Julio Muller. Cuiabá, MT, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Biologia Molecular e Grupo de Pesquisa Imunologia em Malária. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Biologia Molecular e Grupo de Pesquisa Imunologia em Malária. Belo Horizonte, MG, BrasilFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Biologia Molecular e Grupo de Pesquisa Imunologia em Malária. Belo Horizonte, MG, BrasilBackground: Parasite resistance to anti-malarials represents a great obstacle for malaria elimination. The majority of studies have investigated the association between single-nucleotide polymorphisms (SNPs) and drug resistance; however, it is becoming clear that the copy number variation (CNV) is also associated with this parasite phenotype. To provide a baseline for molecular surveillance of anti-malarial drug resistance in the Brazilian Amazon, the present study characterized the genetic profile of both markers in the most common genes associated with drug resistance in Plasmodium falciparum and Plasmodium vivax isolates. Additionally, these data were compared to data published elsewhere applying a systematic review of the literature published over a 20-year time period. Methods: The genomic DNA of 67 patients infected by P. falciparum and P. vivax from three Brazilian States was obtained between 2002 and 2012. CNV in P. falciparum multidrug resistance gene-1 (pfmdr1), GTP cyclohydrolase 1 (pfgch1) and P. vivax multidrug resistance gene-1 (pvmdr1) were assessed by real-time PCR assays. SNPs in the pfmdr1 and pfcrt genes were assessed by PCR–RFLP. A literature search for studies that analysed CNP in the same genes of P. falciparum and P. vivax was conducted between May 2014 and March 2017 across four databases. Results: All analysed samples of P. falciparum carried only one copy of pfmdr1 or pfgch1. Although the pfcrt K76T polymorphism, a determinant of CQ resistance, was present in all samples genotyped, the pfmdr1 N86Y was absent. For P. vivax isolates, an amplification rate of 20% was found for the pvmdr1 gene. The results of the study are in agreement with the low amplification rates for pfmdr1 gene evidenced in the Americas and Africa, while higher rates have been described in Southeast Asia. For P. vivax, very low rates of amplification for pvmdr1 have been described worldwide, with exceptions in French Guiana, Cambodia, Thailand and Brazil. Conclusions: The present study was the first to evaluate gch1 CNV in P. falciparum isolates from Brazil, showing an absence of amplification of this gene more than 20 years after the withdrawal of the Brazilian antifolates therapeutic scheme. Furthermore, the rate of pvmdr1 amplification was significantly higher than that previously reported for isolates circulating in Northern Brazil.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-83082https://www.arca.fiocruz.br/bitstream/icict/25477/1/license.txt9193a7c197bc67acd023525e72a03240MD51ORIGINALAssessment of copy number.pdfAssessment of copy number.pdfapplication/pdf6464124https://www.arca.fiocruz.br/bitstream/icict/25477/2/Assessment%20of%20copy%20number.pdfa8ed91cf94c7e44c8d9e2e7387a1bca6MD52TEXTAssessment of copy number.pdf.txtAssessment of copy number.pdf.txtExtracted texttext/plain588https://www.arca.fiocruz.br/bitstream/icict/25477/3/Assessment%20of%20copy%20number.pdf.txt8ca5f324631e5ae23d6e7cd8e1e4a8d4MD53icict/254772021-03-24 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dc.title.pt_BR.fl_str_mv Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature
title Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature
spellingShingle Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature
Costa, Gabriel Luíz
Malaria
Plasmodium falciparum
Plasmodium vivax
resistencia a drogas
Malaria
Plasmodium falciparum
Plasmodium vivax
Drug resistance
title_short Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature
title_full Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature
title_fullStr Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature
title_full_unstemmed Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature
title_sort Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature
author Costa, Gabriel Luíz
author_facet Costa, Gabriel Luíz
Amaral, Lara Cotta
Fontes, Cor Jesus Fernandes
Carvalho, Luzia Helena de
Brito, Cristiana Ferreira Alves de
Sousa, Taís Nóbrega de
author_role author
author2 Amaral, Lara Cotta
Fontes, Cor Jesus Fernandes
Carvalho, Luzia Helena de
Brito, Cristiana Ferreira Alves de
Sousa, Taís Nóbrega de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Costa, Gabriel Luíz
Amaral, Lara Cotta
Fontes, Cor Jesus Fernandes
Carvalho, Luzia Helena de
Brito, Cristiana Ferreira Alves de
Sousa, Taís Nóbrega de
dc.subject.other.pt_BR.fl_str_mv Malaria
Plasmodium falciparum
Plasmodium vivax
resistencia a drogas
topic Malaria
Plasmodium falciparum
Plasmodium vivax
resistencia a drogas
Malaria
Plasmodium falciparum
Plasmodium vivax
Drug resistance
dc.subject.en.pt_BR.fl_str_mv Malaria
Plasmodium falciparum
Plasmodium vivax
Drug resistance
description 2020-01-01
publishDate 2017
dc.date.issued.fl_str_mv 2017
dc.date.accessioned.fl_str_mv 2018-03-23T18:13:08Z
dc.date.available.fl_str_mv 2018-03-23T18:13:08Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv COSTA, Gabriel Luíz et al. Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature. Malaria Journal, v. 16, art. 152, 2017
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/25477
dc.identifier.issn.pt_BR.fl_str_mv 1475-2875
dc.identifier.doi.none.fl_str_mv 10.1186/s12936-017-1806-z
identifier_str_mv COSTA, Gabriel Luíz et al. Assessment of copy number variation in genes related to drug resistance in Plasmodium vivax and Plasmodium falciparum isolates from the Brazilian Amazon and a systematic review of the literature. Malaria Journal, v. 16, art. 152, 2017
1475-2875
10.1186/s12936-017-1806-z
url https://www.arca.fiocruz.br/handle/icict/25477
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Biomed Central
publisher.none.fl_str_mv Biomed Central
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
instname_str Fundação Oswaldo Cruz (FIOCRUZ)
instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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