Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2004 |
| Outros Autores: | , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/33628 |
Resumo: | Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Departamento de Endemias Samuel Pessoa. Laboratório de Paleoparasitologia. Rio de Janeiro, RJ, Brasil. |
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Terra, Márcia Andreia Barge LouçãoBello, Alexandre RibeiroBastos, Otilio MachadoAmendoeira, Maria Regina ReisCoelho, Janice Mary Chicarino de OliveiraFerreira, Luiz FernandoAraújo, Adauto2019-06-24T12:33:13Z2019-06-24T12:33:13Z2004TERRA, Márcia Andreia Barge Loução et al. Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 2, p. 185-188, Mar. 2004.0074-0276https://www.arca.fiocruz.br/handle/icict/3362810.1590/S0074-027620040002000121678-8060engInstituto Oswaldo CruzDetection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissuesinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Departamento de Endemias Samuel Pessoa. Laboratório de Paleoparasitologia. Rio de Janeiro, RJ, Brasil.Universidade do Estado do Rio de Janeiro. Faculdade de Ciências Médicas. Laboratório de Biologia Molecular. Disciplina de Parasitologia. Rio de Janeiro, RJ, Brasil.Universidade Federal Fluminense. Instituto Biomédico. Departamento de Microbiologia e Parasitologia. Laboratório da Disciplina de Parasitologia. Niterói, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Protozoologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto de Pesquisa Clínica Evandro Chagas. Laboratório de Anatomia Patológica. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Departamento de Endemias Samuel Pessoa. Laboratório de Paleoparasitologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Departamento de Endemias Samuel Pessoa. Laboratório de Paleoparasitologia. Rio de Janeiro, RJ, Brasil.Despite toxoplasmosis being a common infection among human and other warm-blooded animals worldwide, there are no findings about Toxoplasma gondii evolutionary forms in ancient populations. The molecular techniques used for amplification of genetic material have allowed recovery of ancient DNA (aDNA) from parasites contained in mummified tissues. The application of polymerase chain reaction (PCR) to paleoparasitological toxoplasmosis research becomes a promising option, since it might allow diagnosis, acquisition of paleoepidemiological data, access to toxoplasmosis information related origin, evolution, and distribution among the ancient populations. Furthermore, it makes possible the analysis of parasite aDNA aiming at phylogenetic studies. To standardize and evaluate PCR applicability to toxoplasmosis paleodiagnostic, an experimental mummification protocol was tested using desiccated tissues from mice infected with the ME49 strain cysts, the chronic infection group (CIG), or infected with tachyzoites (RH strain), the acute infection group (AIG). Tissues were subjected to DNA extraction followed by PCR amplification of T. gondii B1 gene. PCR recovered T. gondii DNA in thigh muscle, encephalon, heart, and lung samples. AIG presented PCR positivity in encephalon, lungs, hearts, and livers. Based on this results, we propose this molecular approach for toxoplasmosis research in past populations.PaleoparasitologyToxoplasmosisAncient DNAMummiesParasitismToxoplasma gondiiinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1748https://www.arca.fiocruz.br/bitstream/icict/33628/1/license.txt8a4605be74aa9ea9d79846c1fba20a33MD51ORIGINALve_Terra_Márcia_etal_INI_2004.pdfve_Terra_Márcia_etal_INI_2004.pdfapplication/pdf82867https://www.arca.fiocruz.br/bitstream/icict/33628/2/ve_Terra_M%c3%a1rcia_etal_INI_2004.pdf09f29cb3fb8fbd8a98254ce809d965aeMD52TEXTve_Terra_Márcia_etal_INI_2004.pdf.txtve_Terra_Márcia_etal_INI_2004.pdf.txtExtracted texttext/plain22116https://www.arca.fiocruz.br/bitstream/icict/33628/3/ve_Terra_M%c3%a1rcia_etal_INI_2004.pdf.txt533a11763ceea68c7982d2a9f6ad10c3MD53icict/336282019-06-25 02:03:00.919oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-25T05:03Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
| title |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
| spellingShingle |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues Terra, Márcia Andreia Barge Loução Paleoparasitology Toxoplasmosis Ancient DNA Mummies Parasitism Toxoplasma gondii |
| title_short |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
| title_full |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
| title_fullStr |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
| title_full_unstemmed |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
| title_sort |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
| author |
Terra, Márcia Andreia Barge Loução |
| author_facet |
Terra, Márcia Andreia Barge Loução Bello, Alexandre Ribeiro Bastos, Otilio Machado Amendoeira, Maria Regina Reis Coelho, Janice Mary Chicarino de Oliveira Ferreira, Luiz Fernando Araújo, Adauto |
| author_role |
author |
| author2 |
Bello, Alexandre Ribeiro Bastos, Otilio Machado Amendoeira, Maria Regina Reis Coelho, Janice Mary Chicarino de Oliveira Ferreira, Luiz Fernando Araújo, Adauto |
| author2_role |
author author author author author author |
| dc.contributor.author.fl_str_mv |
Terra, Márcia Andreia Barge Loução Bello, Alexandre Ribeiro Bastos, Otilio Machado Amendoeira, Maria Regina Reis Coelho, Janice Mary Chicarino de Oliveira Ferreira, Luiz Fernando Araújo, Adauto |
| dc.subject.en.pt_BR.fl_str_mv |
Paleoparasitology Toxoplasmosis Ancient DNA Mummies Parasitism Toxoplasma gondii |
| topic |
Paleoparasitology Toxoplasmosis Ancient DNA Mummies Parasitism Toxoplasma gondii |
| description |
Fundação Oswaldo Cruz. Escola Nacional de Saúde Pública. Departamento de Endemias Samuel Pessoa. Laboratório de Paleoparasitologia. Rio de Janeiro, RJ, Brasil. |
| publishDate |
2004 |
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2004 |
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2019-06-24T12:33:13Z |
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2019-06-24T12:33:13Z |
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TERRA, Márcia Andreia Barge Loução et al. Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 2, p. 185-188, Mar. 2004. |
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https://www.arca.fiocruz.br/handle/icict/33628 |
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0074-0276 |
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10.1590/S0074-02762004000200012 |
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1678-8060 |
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TERRA, Márcia Andreia Barge Loução et al. Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 99, n. 2, p. 185-188, Mar. 2004. 0074-0276 10.1590/S0074-02762004000200012 1678-8060 |
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