Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues
Autor(a) principal: | |
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Data de Publicação: | 2004 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Memórias do Instituto Oswaldo Cruz |
Texto Completo: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000200012 |
Resumo: | Despite toxoplasmosis being a common infection among human and other warm-blooded animals worldwide, there are no findings about Toxoplasma gondii evolutionary forms in ancient populations. The molecular techniques used for amplification of genetic material have allowed recovery of ancient DNA (aDNA) from parasites contained in mummified tissues. The application of polymerase chain reaction (PCR) to paleoparasitological toxoplasmosis research becomes a promising option, since it might allow diagnosis, acquisition of paleoepidemiological data, access to toxoplasmosis information related origin, evolution, and distribution among the ancient populations.Furthermore, it makes possible the analysis of parasite aDNA aiming at phylogenetic studies. To standardize and evaluate PCR applicability to toxoplasmosis paleodiagnostic, an experimental mummification protocol was tested using desiccated tissues from mice infected with the ME49 strain cysts, the chronic infection group (CIG), or infected with tachyzoites (RH strain), the acute infection group (AIG). Tissues were subjected to DNA extraction followed by PCR amplification of T. gondii B1 gene. PCR recovered T. gondii DNA in thigh muscle, encephalon, heart, and lung samples. AIG presented PCR positivity in encephalon, lungs, hearts, and livers. Based on this results, we propose this molecular approach for toxoplasmosis research in past populations. |
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Memórias do Instituto Oswaldo Cruz |
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Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissuespaleoparasitologytoxoplasmosisancient DNAmummiesparasitismToxoplasma gondiiDespite toxoplasmosis being a common infection among human and other warm-blooded animals worldwide, there are no findings about Toxoplasma gondii evolutionary forms in ancient populations. The molecular techniques used for amplification of genetic material have allowed recovery of ancient DNA (aDNA) from parasites contained in mummified tissues. The application of polymerase chain reaction (PCR) to paleoparasitological toxoplasmosis research becomes a promising option, since it might allow diagnosis, acquisition of paleoepidemiological data, access to toxoplasmosis information related origin, evolution, and distribution among the ancient populations.Furthermore, it makes possible the analysis of parasite aDNA aiming at phylogenetic studies. To standardize and evaluate PCR applicability to toxoplasmosis paleodiagnostic, an experimental mummification protocol was tested using desiccated tissues from mice infected with the ME49 strain cysts, the chronic infection group (CIG), or infected with tachyzoites (RH strain), the acute infection group (AIG). Tissues were subjected to DNA extraction followed by PCR amplification of T. gondii B1 gene. PCR recovered T. gondii DNA in thigh muscle, encephalon, heart, and lung samples. AIG presented PCR positivity in encephalon, lungs, hearts, and livers. Based on this results, we propose this molecular approach for toxoplasmosis research in past populations.Instituto Oswaldo Cruz, Ministério da Saúde2004-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000200012Memórias do Instituto Oswaldo Cruz v.99 n.2 2004reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762004000200012info:eu-repo/semantics/openAccessTerra,Márcia Andreia Barge LouçãoBello,Alexandre RibeiroBastos,Otilio MachadoAmendoeira,Regina ReisCoelho,Janice Mary Chicarino de OliveiraFerreira,Luiz FernandoAraújo,Adautoeng2020-04-25T17:49:13Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:12:31.628Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue |
dc.title.none.fl_str_mv |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
title |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
spellingShingle |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues Terra,Márcia Andreia Barge Loução paleoparasitology toxoplasmosis ancient DNA mummies parasitism Toxoplasma gondii |
title_short |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
title_full |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
title_fullStr |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
title_full_unstemmed |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
title_sort |
Detection of Toxoplasma gondii DNA by polymerase chain reaction in experimentally desiccated tissues |
author |
Terra,Márcia Andreia Barge Loução |
author_facet |
Terra,Márcia Andreia Barge Loução Bello,Alexandre Ribeiro Bastos,Otilio Machado Amendoeira,Regina Reis Coelho,Janice Mary Chicarino de Oliveira Ferreira,Luiz Fernando Araújo,Adauto |
author_role |
author |
author2 |
Bello,Alexandre Ribeiro Bastos,Otilio Machado Amendoeira,Regina Reis Coelho,Janice Mary Chicarino de Oliveira Ferreira,Luiz Fernando Araújo,Adauto |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Terra,Márcia Andreia Barge Loução Bello,Alexandre Ribeiro Bastos,Otilio Machado Amendoeira,Regina Reis Coelho,Janice Mary Chicarino de Oliveira Ferreira,Luiz Fernando Araújo,Adauto |
dc.subject.por.fl_str_mv |
paleoparasitology toxoplasmosis ancient DNA mummies parasitism Toxoplasma gondii |
topic |
paleoparasitology toxoplasmosis ancient DNA mummies parasitism Toxoplasma gondii |
dc.description.none.fl_txt_mv |
Despite toxoplasmosis being a common infection among human and other warm-blooded animals worldwide, there are no findings about Toxoplasma gondii evolutionary forms in ancient populations. The molecular techniques used for amplification of genetic material have allowed recovery of ancient DNA (aDNA) from parasites contained in mummified tissues. The application of polymerase chain reaction (PCR) to paleoparasitological toxoplasmosis research becomes a promising option, since it might allow diagnosis, acquisition of paleoepidemiological data, access to toxoplasmosis information related origin, evolution, and distribution among the ancient populations.Furthermore, it makes possible the analysis of parasite aDNA aiming at phylogenetic studies. To standardize and evaluate PCR applicability to toxoplasmosis paleodiagnostic, an experimental mummification protocol was tested using desiccated tissues from mice infected with the ME49 strain cysts, the chronic infection group (CIG), or infected with tachyzoites (RH strain), the acute infection group (AIG). Tissues were subjected to DNA extraction followed by PCR amplification of T. gondii B1 gene. PCR recovered T. gondii DNA in thigh muscle, encephalon, heart, and lung samples. AIG presented PCR positivity in encephalon, lungs, hearts, and livers. Based on this results, we propose this molecular approach for toxoplasmosis research in past populations. |
description |
Despite toxoplasmosis being a common infection among human and other warm-blooded animals worldwide, there are no findings about Toxoplasma gondii evolutionary forms in ancient populations. The molecular techniques used for amplification of genetic material have allowed recovery of ancient DNA (aDNA) from parasites contained in mummified tissues. The application of polymerase chain reaction (PCR) to paleoparasitological toxoplasmosis research becomes a promising option, since it might allow diagnosis, acquisition of paleoepidemiological data, access to toxoplasmosis information related origin, evolution, and distribution among the ancient populations.Furthermore, it makes possible the analysis of parasite aDNA aiming at phylogenetic studies. To standardize and evaluate PCR applicability to toxoplasmosis paleodiagnostic, an experimental mummification protocol was tested using desiccated tissues from mice infected with the ME49 strain cysts, the chronic infection group (CIG), or infected with tachyzoites (RH strain), the acute infection group (AIG). Tissues were subjected to DNA extraction followed by PCR amplification of T. gondii B1 gene. PCR recovered T. gondii DNA in thigh muscle, encephalon, heart, and lung samples. AIG presented PCR positivity in encephalon, lungs, hearts, and livers. Based on this results, we propose this molecular approach for toxoplasmosis research in past populations. |
publishDate |
2004 |
dc.date.none.fl_str_mv |
2004-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000200012 |
url |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762004000200012 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S0074-02762004000200012 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
publisher.none.fl_str_mv |
Instituto Oswaldo Cruz, Ministério da Saúde |
dc.source.none.fl_str_mv |
Memórias do Instituto Oswaldo Cruz v.99 n.2 2004 reponame:Memórias do Instituto Oswaldo Cruz instname:Fundação Oswaldo Cruz instacron:FIOCRUZ |
reponame_str |
Memórias do Instituto Oswaldo Cruz |
collection |
Memórias do Instituto Oswaldo Cruz |
instname_str |
Fundação Oswaldo Cruz |
instacron_str |
FIOCRUZ |
institution |
FIOCRUZ |
repository.name.fl_str_mv |
Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz |
repository.mail.fl_str_mv |
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1669937690331578368 |