HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
Texto Completo: | https://www.arca.fiocruz.br/handle/icict/26766 |
Resumo: | Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). |
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Cunha, Joana Paixão MonteiroSouza, Juliana Sacramento Mota deDiaz, Ricardo SobhieRego, Filipe Ferreira de AlmeidaAndrade, ESilva, Márcio de OliveiraMarta, GiovanettiOliveira, T. deOliveira, AAlves, Carlos Roberto BritesAlcantara, Luiz Carlos Júnior2018-06-07T14:08:10Z2018-06-07T14:08:10Z2017CUNHA, J. P. M. et al. HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals. Journal of AIDS and HIV Infections, v.3, n. 1, p. 103, 2017.2454-499Xhttps://www.arca.fiocruz.br/handle/icict/26766Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq).Federal University of Bahia. Core Bioinformatics Department Bioformatics. Salvador, BA, Brazil / Bahia School of Medicine and Public Health. Bahia Foundation for the Development of Sciences. Salvador, BA, BrazilFederal University of Bahia. Core Bioinformatics Department Bioformatics. Salvador, BA, BrazilFederal University of São Paulo. Department of Medicine. São Paulo, SP, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, BrasilUniversity of KwaZulu-Natal. Africa Centre for Health and Population Studies. Durban, South AfricaState Center Specialized in Diagnosis. Assistance and Research. Salvador, BA, BrazilFederal University of Bahia. Professor Edgard Santos University Hospital Complex. Laboratory of Infectology Research. Salvador, BA, BrazilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Laboratório de Hematologia, Genética e Biologia Computacional. Salvador, BA, Brasil499XThe distribution of different human immunodeficiency virus type 1 (HIV-1) genotypes and the prevalence of transmitted drug resistance (TDR) mutations vary greatly across different Brazilian regions. This study aimed to describe the HIV-1 molecular diversity and TDR prevalence among treatment-naïve HIV-1 infected individuals in an urban area of Northeastern Brazil. DNA samples from 97 infected individuals were obtained and pol sequences were generated by Polimerase Chain Reaction (PCR) and direct sequencing. Bioinformatics tools were used to identify the presence of associated mutations with drug resistance, to reconstruct the phylogeny and to detect recombination. The median age and estimated time of HIV-1 diagnosis were 34 years and 12 months, respectively and 61% of the patients were male. The main exposure category was sexual (87.5%) and the median TCD4+ lymphocyte counts and viral load were 438 cells/mm3 and 4.4 log10, respectively. The overall TDR prevalence was 5.1% while within each drug class (Non-nucleoside reverse-transcriptase inhibitors - NNRTIs, Nucleoside analog reverse-transcriptase inhibitors - NRTIs and Protease inhibitors - PIs) this rate was 2.1% individually. These TDR mutations confer intermediate to high-level resistance to different drugs that are used as first-line treatment options, which reinforce the importance of genotype testing prior to treatment initia-tion. Phylogenetic analysis showed that 73.2% of the samples were subtype B, 14.4% were subtype F, 4.1% were subtype C and 8.3% were BF recombinants. Our data indicate that the genotypic profile of HIV-1 strains circulating in Bahia is diversified and show an increase of subtype C prevalence in Northeast BrazilengAnnex PublishersGenotipagemMutaçãoAntirretroviralSubtiposHIVResistênciaIntrodução à PrevalênciaGenotypingMutationAntiretroviralSubtypesHIVResistancePrevalence IntroductionHIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individualsinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/26766/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALCunha JPM HIV-1-Molecular-Characterization-and-Transmitted-Drug-Resistance-Prevalence-among-Treatment-Naive-Individuals.pdfCunha JPM 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dc.title.pt_BR.fl_str_mv |
HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals |
title |
HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals |
spellingShingle |
HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals Cunha, Joana Paixão Monteiro Genotipagem Mutação Antirretroviral Subtipos HIV Resistência Introdução à Prevalência Genotyping Mutation Antiretroviral Subtypes HIV Resistance Prevalence Introduction |
title_short |
HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals |
title_full |
HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals |
title_fullStr |
HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals |
title_full_unstemmed |
HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals |
title_sort |
HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals |
author |
Cunha, Joana Paixão Monteiro |
author_facet |
Cunha, Joana Paixão Monteiro Souza, Juliana Sacramento Mota de Diaz, Ricardo Sobhie Rego, Filipe Ferreira de Almeida Andrade, E Silva, Márcio de Oliveira Marta, Giovanetti Oliveira, T. de Oliveira, A Alves, Carlos Roberto Brites Alcantara, Luiz Carlos Júnior |
author_role |
author |
author2 |
Souza, Juliana Sacramento Mota de Diaz, Ricardo Sobhie Rego, Filipe Ferreira de Almeida Andrade, E Silva, Márcio de Oliveira Marta, Giovanetti Oliveira, T. de Oliveira, A Alves, Carlos Roberto Brites Alcantara, Luiz Carlos Júnior |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Cunha, Joana Paixão Monteiro Souza, Juliana Sacramento Mota de Diaz, Ricardo Sobhie Rego, Filipe Ferreira de Almeida Andrade, E Silva, Márcio de Oliveira Marta, Giovanetti Oliveira, T. de Oliveira, A Alves, Carlos Roberto Brites Alcantara, Luiz Carlos Júnior |
dc.subject.other.pt_BR.fl_str_mv |
Genotipagem Mutação Antirretroviral Subtipos HIV Resistência Introdução à Prevalência |
topic |
Genotipagem Mutação Antirretroviral Subtipos HIV Resistência Introdução à Prevalência Genotyping Mutation Antiretroviral Subtypes HIV Resistance Prevalence Introduction |
dc.subject.en.pt_BR.fl_str_mv |
Genotyping Mutation Antiretroviral Subtypes HIV Resistance Prevalence Introduction |
description |
Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). |
publishDate |
2017 |
dc.date.issued.fl_str_mv |
2017 |
dc.date.accessioned.fl_str_mv |
2018-06-07T14:08:10Z |
dc.date.available.fl_str_mv |
2018-06-07T14:08:10Z |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
format |
article |
status_str |
publishedVersion |
dc.identifier.citation.fl_str_mv |
CUNHA, J. P. M. et al. HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals. Journal of AIDS and HIV Infections, v.3, n. 1, p. 103, 2017. |
dc.identifier.uri.fl_str_mv |
https://www.arca.fiocruz.br/handle/icict/26766 |
dc.identifier.issn.pt_BR.fl_str_mv |
2454-499X |
identifier_str_mv |
CUNHA, J. P. M. et al. HIV-1 Molecular Characterization and Transmitted Drug Resistance Prevalence among Treatment-Naïve Individuals. Journal of AIDS and HIV Infections, v.3, n. 1, p. 103, 2017. 2454-499X |
url |
https://www.arca.fiocruz.br/handle/icict/26766 |
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eng |
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eng |
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info:eu-repo/semantics/openAccess |
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openAccess |
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Annex Publishers |
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Annex Publishers |
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