Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling

Detalhes bibliográficos
Autor(a) principal: Blank, Walter A
Data de Publicação: 2009
Outros Autores: Reis, Eliana Almeida Gomes, Thiong, Fred W, Braghiroli, Joao F, Santos, Jarbas M, Melo, Paulo Roberto Santana, Guimarães, Isabel C. S, Silva, Luciano Kalabric, Carmo, Theomira Mauadie de Azevedo, Reis, Mitermayer Galvão dos, Blanton, Ronald Edward
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/14915
Resumo: Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
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spelling Blank, Walter AReis, Eliana Almeida GomesThiong, Fred WBraghiroli, Joao FSantos, Jarbas MMelo, Paulo Roberto SantanaGuimarães, Isabel C. SSilva, Luciano KalabricCarmo, Theomira Mauadie de AzevedoReis, Mitermayer Galvão dosBlanton, Ronald Edward2016-07-05T17:02:47Z2016-07-14T19:07:31Z2016-07-05T17:02:47Z2016-07-14T19:07:31Z2009BLANK, W. A. et al. Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling. Journal of Parasitology, v. 95, n. 4, p. 881–889, 2009.0022-3395https://www.arca.fiocruz.br/handle/icict/14915engAmerican Society of ParasitologistsDirect analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg samplinginfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversity of Nairobi. Nairobi, KenyaFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilUniversidade Federal da Bahia. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilFundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, BrasilCase Western Reserve University. Center for Global Health and Diseases. Cleveland, OhioMany parasite populations are difficult to sample because they are non-uniformly distributed between several host species and are often not easily collected from the living host, limiting sample size and possibly distorting the representation of the population. For the parasite Schistosoma mansoni, we investigated the use of the aggregated eggs found in the stool of infected individuals as a simple and representative sample. Previously, we demonstrated that microsatellite allele frequencies can be accurately estimated from pooled DNA of cloned S. mansoni adults, and we show here that genotyping parasite populations from reproductively isolated laboratory strains can be used to identify these specific populations based on characteristic patterns of allele frequencies, as observed by polyacrylamide gel electrophoresis and automated sequencer analysis of fluorescently labeled PCR products. In addition, microsatellites used to genotype aggregates of eggs collected from stools of infected individuals produced results consistent with the geographic distribution of the samples. Direct analysis of total stool eggs can be an important approach to questions of population genetics for this parasite by increasing the sample size to thousands per individual and reducing bias.Schistosoma mansoniParasitesGenotypeMicrosatelliteElectrophoresisGenetics, PopulationHumansinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZTEXTBlank W A Direct analysis of .....pdf.txtBlank W A Direct analysis of .....pdf.txtExtracted texttext/plain38538https://www.arca.fiocruz.br/bitstream/icict/14915/4/Blank%20W%20A%20Direct%20analysis%20of%20.....pdf.txtae8857339164d03216b6ee8ced883e08MD54ORIGINALBlank W A Direct analysis of 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dc.title.pt_BR.fl_str_mv Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling
title Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling
spellingShingle Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling
Blank, Walter A
Schistosoma mansoni
Parasites
Genotype
Microsatellite
Electrophoresis
Genetics, Population
Humans
title_short Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling
title_full Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling
title_fullStr Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling
title_full_unstemmed Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling
title_sort Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling
author Blank, Walter A
author_facet Blank, Walter A
Reis, Eliana Almeida Gomes
Thiong, Fred W
Braghiroli, Joao F
Santos, Jarbas M
Melo, Paulo Roberto Santana
Guimarães, Isabel C. S
Silva, Luciano Kalabric
Carmo, Theomira Mauadie de Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
author_role author
author2 Reis, Eliana Almeida Gomes
Thiong, Fred W
Braghiroli, Joao F
Santos, Jarbas M
Melo, Paulo Roberto Santana
Guimarães, Isabel C. S
Silva, Luciano Kalabric
Carmo, Theomira Mauadie de Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Blank, Walter A
Reis, Eliana Almeida Gomes
Thiong, Fred W
Braghiroli, Joao F
Santos, Jarbas M
Melo, Paulo Roberto Santana
Guimarães, Isabel C. S
Silva, Luciano Kalabric
Carmo, Theomira Mauadie de Azevedo
Reis, Mitermayer Galvão dos
Blanton, Ronald Edward
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni
Parasites
Genotype
Microsatellite
Electrophoresis
Genetics, Population
Humans
topic Schistosoma mansoni
Parasites
Genotype
Microsatellite
Electrophoresis
Genetics, Population
Humans
description Fundação Gonçalo Moniz. Centro de Pesquisas Gonçalo Moniz. Salvador, BA, Brasil
publishDate 2009
dc.date.issued.fl_str_mv 2009
dc.date.accessioned.fl_str_mv 2016-07-05T17:02:47Z
2016-07-14T19:07:31Z
dc.date.available.fl_str_mv 2016-07-05T17:02:47Z
2016-07-14T19:07:31Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
format article
status_str publishedVersion
dc.identifier.citation.fl_str_mv BLANK, W. A. et al. Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling. Journal of Parasitology, v. 95, n. 4, p. 881–889, 2009.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/14915
dc.identifier.issn.pt_BR.fl_str_mv 0022-3395
identifier_str_mv BLANK, W. A. et al. Direct analysis of Schistosoma Mansoni population structure using aggregated individuals from laboratory strains and total fecal egg sampling. Journal of Parasitology, v. 95, n. 4, p. 881–889, 2009.
0022-3395
url https://www.arca.fiocruz.br/handle/icict/14915
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv American Society of Parasitologists
publisher.none.fl_str_mv American Society of Parasitologists
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
instname:Fundação Oswaldo Cruz (FIOCRUZ)
instacron:FIOCRUZ
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instacron_str FIOCRUZ
institution FIOCRUZ
reponame_str Repositório Institucional da FIOCRUZ (ARCA)
collection Repositório Institucional da FIOCRUZ (ARCA)
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