Interaction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite

Detalhes bibliográficos
Autor(a) principal: Mattos, Ana Carolina Alves de
Data de Publicação: 2011
Outros Autores: Souza, Raquel Lopes Martins, Kusel, John Robert, Coelho, Paulo Marcos Zech
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/8670
Resumo: Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
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spelling Mattos, Ana Carolina Alves deSouza, Raquel Lopes MartinsKusel, John RobertCoelho, Paulo Marcos Zech2014-10-24T11:38:46Z2014-10-24T11:38:46Z2011MATTOS, Ana Carolina Alves de et al. Interaction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite. Memorias do Instituto Oswaldo Cruz. 2011; 106(4): 424-432.0074-0276https://www.arca.fiocruz.br/handle/icict/86700074-0276 10.1590/S0074-02762011000400007engFundação Oswaldo CruzInteraction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasiteinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilUniversidade Federal de Alfenas. Laboratório de Parasitologia. Alfenas, MG, BrasilUniversity of Glasgow. Division of Infection and Immunity. Scotland, UKFundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, BrasilThe outcome of the interaction between Biomphalaria and Schistosoma mansoni depends on the response of the host internal defence system (IDS) and the escape mechanisms of the parasite. The aim of this study was to evaluate the responsiveness of the IDS (haemocytes and soluble haemolymph factors) of resistant and susceptible Biomphalaria tenagophila lineages and Biomphalaria glabrata lineages in the presence of in vitro-transformed primary sporocysts and secondary sporocysts obtained from infected B. glabrata. To do this, we assayed the cellular adhesion index (CAI), analysed viability/mortality, used fluorescent markers to evaluate the tegumental damage and transplanted secondary sporocysts. B. tenagophila Taim was more effective against primary and secondary sporocystes than the susceptible lineage and B. glabrata. Compared with secondary sporocysts exposed to B. tenagophila, primary sporocysts showed a higher CAI, a greater percentage of dead sporocysts and were labelled by lectin from Glycine max and Alexa-Fluor 488 fluorescent probes at a higher rate than the secondary sporocysts. However, the two B. tenagophila lineages showed no cercarial shedding after inoculation with secondary sporocysts. Our hypothesis that secondary sporocysts can escape the B. tenagophila IDS cannot be confirmed by the transplantation experiments. These data suggest that there are additional mechanisms involved in the lower susceptibilty of B. tenagophila to S. mansoni infection.Schistosoma mansoni/sporocystsBiomphalaria tenagophila/internal defence systemBiomphalaria tenagophila/escape mechanismsinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALInteraction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite.pdfInteraction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite.pdfapplication/pdf1257614https://www.arca.fiocruz.br/bitstream/icict/8670/2/Interaction%20between%20primary%20and%20secondary%20sporocysts%20of%20Schistosoma%20mansoni%20and%20internal%20defence%20system%20of%20Biomphalaria%20resistat%20and%20susceptible%20to%20the%20parasite.pdfb079bae4a0237f7132b6bd1b95bc39cdMD52LICENSElicense.txtlicense.txttext/plain; charset=utf-81914https://www.arca.fiocruz.br/bitstream/icict/8670/3/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD53TEXTInteraction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite.pdf.txtInteraction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite.pdf.txtExtracted texttext/plain43450https://www.arca.fiocruz.br/bitstream/icict/8670/4/Interaction%20between%20primary%20and%20secondary%20sporocysts%20of%20Schistosoma%20mansoni%20and%20internal%20defence%20system%20of%20Biomphalaria%20resistat%20and%20susceptible%20to%20the%20parasite.pdf.txtd72a209f0a131ce8c9a5f5bee62da7c2MD54icict/86702019-06-19 10:12:21.73oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352019-06-19T13:12:21Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false
dc.title.pt_BR.fl_str_mv Interaction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite
title Interaction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite
spellingShingle Interaction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite
Mattos, Ana Carolina Alves de
Schistosoma mansoni/sporocysts
Biomphalaria tenagophila/internal defence system
Biomphalaria tenagophila/escape mechanisms
title_short Interaction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite
title_full Interaction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite
title_fullStr Interaction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite
title_full_unstemmed Interaction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite
title_sort Interaction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite
author Mattos, Ana Carolina Alves de
author_facet Mattos, Ana Carolina Alves de
Souza, Raquel Lopes Martins
Kusel, John Robert
Coelho, Paulo Marcos Zech
author_role author
author2 Souza, Raquel Lopes Martins
Kusel, John Robert
Coelho, Paulo Marcos Zech
author2_role author
author
author
dc.contributor.author.fl_str_mv Mattos, Ana Carolina Alves de
Souza, Raquel Lopes Martins
Kusel, John Robert
Coelho, Paulo Marcos Zech
dc.subject.en.pt_BR.fl_str_mv Schistosoma mansoni/sporocysts
Biomphalaria tenagophila/internal defence system
Biomphalaria tenagophila/escape mechanisms
topic Schistosoma mansoni/sporocysts
Biomphalaria tenagophila/internal defence system
Biomphalaria tenagophila/escape mechanisms
description Fundação Oswaldo Cruz. Centro de Pesquisas René Rachou. Laboratório de Esquistossomose. Belo Horizonte, MG, Brasil
publishDate 2011
dc.date.issued.fl_str_mv 2011
dc.date.accessioned.fl_str_mv 2014-10-24T11:38:46Z
dc.date.available.fl_str_mv 2014-10-24T11:38:46Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv MATTOS, Ana Carolina Alves de et al. Interaction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite. Memorias do Instituto Oswaldo Cruz. 2011; 106(4): 424-432.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/8670
dc.identifier.issn.none.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 0074-0276 10.1590/S0074-02762011000400007
identifier_str_mv MATTOS, Ana Carolina Alves de et al. Interaction between primary and secondary sporocysts of Schistosoma mansoni and internal defence system of Biomphalaria resistat and susceptible to the parasite. Memorias do Instituto Oswaldo Cruz. 2011; 106(4): 424-432.
0074-0276
0074-0276 10.1590/S0074-02762011000400007
url https://www.arca.fiocruz.br/handle/icict/8670
dc.language.iso.fl_str_mv eng
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dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz
publisher.none.fl_str_mv Fundação Oswaldo Cruz
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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