Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2005 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/3240 |
Resumo: | Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil |
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Favali, Cecilia Beatriz FiuzaCosta, Dirceu JoaquimAfonso, Lilian MariaConceição, VivianeRosato, Andréa BomuraOliveira, FabianoCosta, Jackson Mauricio LopesBarral, Aldina Maria PradoBarral Netto, ManoelBrodskyn, Claudia Ida2011-09-15T18:28:41Z2011-09-15T18:28:41Z2005FAVALI, C. et al. Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis. Microbes and Infection, v.7, n.1, p.86-92, jan. 2005.1286-4579https://www.arca.fiocruz.br/handle/icict/3240doi:10.1016/j.micinf.2004.09.015engRole of costimulatory molecules in immune response of patients with cutaneous leishmaniasisinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilFundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, BrasilT cell-mediated immunity is critical in resistance against Leishmania parasites, and T cell activation requires signals provided by costimulatory molecules. Herein we evaluated the role of costimulatory molecules on cytokine production and T cell surface molecule expression by peripheral blood mononuclear cells (PBMC) from cutaneous leishmaniasis (CL) patients. PBMC from CL patients were stimulated with soluble Leishmania antigen (SLA, 10 microg/ml), in the presence or absence of soluble CTLA4-Ig to block CD28-B7 interaction or in the presence or absence of anti-human CD40L to block CD40-CD40L interaction. Supernatants were harvested to evaluate tumor necrosis factor alpha (TNF-alpha), interleukin 10 (IL-10), transforming growth factor beta (TGF-beta) and interferon gamma (IFN-gamma) production by ELISA. Cells were harvested after 48 h of culture, stained for specific activation markers and analyzed by flow cytometry. Results show that the blockade of CD28-B7 interaction by CTLA4-Ig downmodulated IFN-gamma, IL-10, and TNF-alpha secretion by PBMC from CL patients. No alteration was detected on either TGF-beta production or the expression of CTLA44 or CD25 on CD4+ and CD8+ T cells. When the CD40-CD40L interaction was blockade using anti-CD40L, we did not observe changes in cytokine production or in surface molecule expression. The blockade of the CD28-B7 interactions by CTLA4-Ig also did not alter cytokine production in volunteers immunized against tetanus toxoid (TT). Taken together, these data suggest that the interaction of CTLA4 and CD28-B7 is a TGF-beta-independent mechanism that specifically downmodulates the immune response in cutaneous leishmaniasis patients.Cutaneous leishmaniasisCytokinesImmunomodulationAntígenosCélulas CultivadasRegulação para BaixoImunoconjugadosInterferon gamaInterleucina-10Leishmaniose CutâneaLeucócitos MononuclearesLinfócitos TFator de Necrose Tumoral alfaFemininoHumanosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZORIGINALFavali C Role of costimulatory molecules....pdfFavali C Role of costimulatory molecules....pdfapplication/pdf341415https://www.arca.fiocruz.br/bitstream/icict/3240/1/Favali%20C%20Role%20of%20costimulatory%20molecules....pdffccb7e6784b27068e4cb1db75a3f9a98MD51LICENSElicense.txtlicense.txttext/plain; charset=utf-81648https://www.arca.fiocruz.br/bitstream/icict/3240/2/license.txte095249ac7cacefbfe39684dfe45e706MD52TEXTFavali C Role of costimulatory molecules....pdf.txtFavali C Role of costimulatory molecules....pdf.txtExtracted texttext/plain29171https://www.arca.fiocruz.br/bitstream/icict/3240/5/Favali%20C%20Role%20of%20costimulatory%20molecules....pdf.txtd04756727ba8ba11e53ed2a63125b7d6MD55THUMBNAILFavali C Role of costimulatory molecules....pdf.jpgFavali C Role of costimulatory molecules....pdf.jpgGenerated Thumbnailimage/jpeg1719https://www.arca.fiocruz.br/bitstream/icict/3240/4/Favali%20C%20Role%20of%20costimulatory%20molecules....pdf.jpg3ef6869470b00b7ba25a79a6636f0862MD54icict/32402023-03-15 14:33:19.227oai:www.arca.fiocruz.br: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Repositório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352023-03-15T17:33:19Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis |
| title |
Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis |
| spellingShingle |
Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis Favali, Cecilia Beatriz Fiuza Cutaneous leishmaniasis Cytokines Immunomodulation Antígenos Células Cultivadas Regulação para Baixo Imunoconjugados Interferon gama Interleucina-10 Leishmaniose Cutânea Leucócitos Mononucleares Linfócitos T Fator de Necrose Tumoral alfa Feminino Humanos |
| title_short |
Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis |
| title_full |
Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis |
| title_fullStr |
Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis |
| title_full_unstemmed |
Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis |
| title_sort |
Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis |
| author |
Favali, Cecilia Beatriz Fiuza |
| author_facet |
Favali, Cecilia Beatriz Fiuza Costa, Dirceu Joaquim Afonso, Lilian Maria Conceição, Viviane Rosato, Andréa Bomura Oliveira, Fabiano Costa, Jackson Mauricio Lopes Barral, Aldina Maria Prado Barral Netto, Manoel Brodskyn, Claudia Ida |
| author_role |
author |
| author2 |
Costa, Dirceu Joaquim Afonso, Lilian Maria Conceição, Viviane Rosato, Andréa Bomura Oliveira, Fabiano Costa, Jackson Mauricio Lopes Barral, Aldina Maria Prado Barral Netto, Manoel Brodskyn, Claudia Ida |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Favali, Cecilia Beatriz Fiuza Costa, Dirceu Joaquim Afonso, Lilian Maria Conceição, Viviane Rosato, Andréa Bomura Oliveira, Fabiano Costa, Jackson Mauricio Lopes Barral, Aldina Maria Prado Barral Netto, Manoel Brodskyn, Claudia Ida |
| dc.subject.en.pt_BR.fl_str_mv |
Cutaneous leishmaniasis Cytokines Immunomodulation |
| topic |
Cutaneous leishmaniasis Cytokines Immunomodulation Antígenos Células Cultivadas Regulação para Baixo Imunoconjugados Interferon gama Interleucina-10 Leishmaniose Cutânea Leucócitos Mononucleares Linfócitos T Fator de Necrose Tumoral alfa Feminino Humanos |
| dc.subject.decs.pt_BR.fl_str_mv |
Antígenos Células Cultivadas Regulação para Baixo Imunoconjugados Interferon gama Interleucina-10 Leishmaniose Cutânea Leucócitos Mononucleares Linfócitos T Fator de Necrose Tumoral alfa Feminino Humanos |
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Fundação Oswaldo Cruz. Centro de Pesquisas Gonçalo Moniz. Salvador, Bahia, Brasil |
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2005 |
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2005 |
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2011-09-15T18:28:41Z |
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2011-09-15T18:28:41Z |
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FAVALI, C. et al. Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis. Microbes and Infection, v.7, n.1, p.86-92, jan. 2005. |
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1286-4579 |
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doi:10.1016/j.micinf.2004.09.015 |
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FAVALI, C. et al. Role of costimulatory molecules in immune response of patients with cutaneous leishmaniasis. Microbes and Infection, v.7, n.1, p.86-92, jan. 2005. 1286-4579 doi:10.1016/j.micinf.2004.09.015 |
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