Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain

Detalhes bibliográficos
Autor(a) principal: Santos, Flavia Barreto dos
Data de Publicação: 2002
Outros Autores: Miagostovich, Marize Pereira, Nogueira, Rita Maria Ribeiro, Edgil, Dianna, Schatzmayr, Hermann Gonçalves, Riley, Lee W., Harris, Eva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Repositório Institucional da FIOCRUZ (ARCA)
Texto Completo: https://www.arca.fiocruz.br/handle/icict/44225
Resumo: Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
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spelling Santos, Flavia Barreto dosMiagostovich, Marize PereiraNogueira, Rita Maria RibeiroEdgil, DiannaSchatzmayr, Hermann GonçalvesRiley, Lee W.Harris, Eva2020-10-27T14:07:27Z2020-10-27T14:07:27Z2002SANTOS, Flavia Barreto dos et al. Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n.7, p. 991-995, 2002.0074-0276https://www.arca.fiocruz.br/handle/icict/4422510.1590/S0074-027620020007000111678-8060engFundação Oswaldo Cruz. Instituto Oswaldo Cruz.Vírus da Dengue tipo 2Genoma completoBrasilDengue virus type 2Complete genomeBrazilComplete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Straininfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.University of California. School of Public Health. Division of Infectious Diseases. Berkeley, CA, USA.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.University of California. School of Public Health. Division of Infectious Diseases. Berkeley, CA, USA.University of California. School of Public Health. Division of Infectious Diseases. Berkeley, CA, USA.In the last decade, dengue fever (DF) in Brazil has been recognized as an important public health problem, and an increasing number of dengue haemorrhagic fever (DHF) cases have been reported since the introduction of dengue virus type 2 (DEN-2) into the country in 1990. In order to analyze the complete genome sequence of a DEN2 Brazilian strain (BR64022/98), we designed primers to amplify contiguous segments of approximately 500 base pairs across the entire sequence of the viral genome. Twenty fragments amplified by reverse transcriptase-PCR were cloned, and the complete nucleotide and the deduced amino acid sequences were determined. This constitutes the first complete genetic characterization of a DEN-2 strain from Brazil. All amino acid changes differentiating strains related to the Asian/American-Asian genotype were observed in BR64022/98, indicating the Asiatic origin of the strain.info:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txtlicense.txttext/plain; charset=utf-82991https://www.arca.fiocruz.br/bitstream/icict/44225/1/license.txt5a560609d32a3863062d77ff32785d58MD51ORIGINALFlaviaBSantos_RtiaMNogueira_etal_IOC_2002.pdfFlaviaBSantos_RtiaMNogueira_etal_IOC_2002.pdfapplication/pdf54851https://www.arca.fiocruz.br/bitstream/icict/44225/2/FlaviaBSantos_RtiaMNogueira_etal_IOC_2002.pdf2c4db25e30833a493da0e602289801d7MD52TEXTFlaviaBSantos_RtiaMNogueira_etal_IOC_2002.pdf.txtFlaviaBSantos_RtiaMNogueira_etal_IOC_2002.pdf.txtExtracted 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dc.title.pt_BR.fl_str_mv Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain
title Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain
spellingShingle Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain
Santos, Flavia Barreto dos
Vírus da Dengue tipo 2
Genoma completo
Brasil
Dengue virus type 2
Complete genome
Brazil
title_short Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain
title_full Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain
title_fullStr Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain
title_full_unstemmed Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain
title_sort Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain
author Santos, Flavia Barreto dos
author_facet Santos, Flavia Barreto dos
Miagostovich, Marize Pereira
Nogueira, Rita Maria Ribeiro
Edgil, Dianna
Schatzmayr, Hermann Gonçalves
Riley, Lee W.
Harris, Eva
author_role author
author2 Miagostovich, Marize Pereira
Nogueira, Rita Maria Ribeiro
Edgil, Dianna
Schatzmayr, Hermann Gonçalves
Riley, Lee W.
Harris, Eva
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos, Flavia Barreto dos
Miagostovich, Marize Pereira
Nogueira, Rita Maria Ribeiro
Edgil, Dianna
Schatzmayr, Hermann Gonçalves
Riley, Lee W.
Harris, Eva
dc.subject.other.pt_BR.fl_str_mv Vírus da Dengue tipo 2
Genoma completo
Brasil
topic Vírus da Dengue tipo 2
Genoma completo
Brasil
Dengue virus type 2
Complete genome
Brazil
dc.subject.en.pt_BR.fl_str_mv Dengue virus type 2
Complete genome
Brazil
description Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Departamento de Virologia. Rio de Janeiro, RJ, Brasil.
publishDate 2002
dc.date.issued.fl_str_mv 2002
dc.date.accessioned.fl_str_mv 2020-10-27T14:07:27Z
dc.date.available.fl_str_mv 2020-10-27T14:07:27Z
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
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status_str publishedVersion
dc.identifier.citation.fl_str_mv SANTOS, Flavia Barreto dos et al. Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n.7, p. 991-995, 2002.
dc.identifier.uri.fl_str_mv https://www.arca.fiocruz.br/handle/icict/44225
dc.identifier.issn.pt_BR.fl_str_mv 0074-0276
dc.identifier.doi.none.fl_str_mv 10.1590/S0074-02762002000700011
dc.identifier.eissn.none.fl_str_mv 1678-8060
identifier_str_mv SANTOS, Flavia Barreto dos et al. Complete Nucleotide Sequence Analysis of a Brazilian Dengue Virus Type 2 Strain. Memórias do Instituto Oswaldo Cruz, Rio de Janeiro, v. 97, n.7, p. 991-995, 2002.
0074-0276
10.1590/S0074-02762002000700011
1678-8060
url https://www.arca.fiocruz.br/handle/icict/44225
dc.language.iso.fl_str_mv eng
language eng
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
publisher.none.fl_str_mv Fundação Oswaldo Cruz. Instituto Oswaldo Cruz.
dc.source.none.fl_str_mv reponame:Repositório Institucional da FIOCRUZ (ARCA)
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instacron_str FIOCRUZ
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