Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strain

Detalhes bibliográficos
Autor(a) principal: Santos,Flávia Barreto dos
Data de Publicação: 2002
Outros Autores: Miagostovich,Marize Pereira, Nogueira,Rita Maria Ribeiro, Edgil,Dianna, Schatzmayr,Hermann Gonçalves, Riley,Lee W, Harris,Eva
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Memórias do Instituto Oswaldo Cruz
Texto Completo: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000700011
Resumo: In the last decade, dengue fever (DF) in Brazil has been recognized as an important public health problem, and an increasing number of dengue haemorrhagic fever (DHF) cases have been reported since the introduction of dengue virus type 2 (DEN-2) into the country in 1990. In order to analyze the complete genome sequence of a DEN-2 Brazilian strain (BR64022/98), we designed primers to amplify contiguous segments of approximately 500 base pairs across the entire sequence of the viral genome. Twenty fragments amplified by reverse transcriptase-PCR were cloned, and the complete nucleotide and the deduced amino acid sequences were determined. This constitutes the first complete genetic characterization of a DEN-2 strain from Brazil. All amino acid changes differentiating strains related to the Asian/American-Asian genotype were observed in BR64022/98, indicating the Asiatic origin of the strain.
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spelling Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 straindengue virus type 2complete genomeBrazilIn the last decade, dengue fever (DF) in Brazil has been recognized as an important public health problem, and an increasing number of dengue haemorrhagic fever (DHF) cases have been reported since the introduction of dengue virus type 2 (DEN-2) into the country in 1990. In order to analyze the complete genome sequence of a DEN-2 Brazilian strain (BR64022/98), we designed primers to amplify contiguous segments of approximately 500 base pairs across the entire sequence of the viral genome. Twenty fragments amplified by reverse transcriptase-PCR were cloned, and the complete nucleotide and the deduced amino acid sequences were determined. This constitutes the first complete genetic characterization of a DEN-2 strain from Brazil. All amino acid changes differentiating strains related to the Asian/American-Asian genotype were observed in BR64022/98, indicating the Asiatic origin of the strain.Instituto Oswaldo Cruz, Ministério da Saúde2002-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000700011Memórias do Instituto Oswaldo Cruz v.97 n.7 2002reponame:Memórias do Instituto Oswaldo Cruzinstname:Fundação Oswaldo Cruzinstacron:FIOCRUZ10.1590/S0074-02762002000700011info:eu-repo/semantics/openAccessSantos,Flávia Barreto dosMiagostovich,Marize PereiraNogueira,Rita Maria RibeiroEdgil,DiannaSchatzmayr,Hermann GonçalvesRiley,Lee WHarris,Evaeng2020-04-25T17:48:52Zhttp://www.scielo.br/oai/scielo-oai.php0074-02761678-8060opendoar:null2020-04-26 02:11:24.86Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruztrue
dc.title.none.fl_str_mv Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strain
title Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strain
spellingShingle Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strain
Santos,Flávia Barreto dos
dengue virus type 2
complete genome
Brazil
title_short Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strain
title_full Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strain
title_fullStr Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strain
title_full_unstemmed Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strain
title_sort Complete nucleotide sequence analysis of a Brazilian dengue virus type 2 strain
author Santos,Flávia Barreto dos
author_facet Santos,Flávia Barreto dos
Miagostovich,Marize Pereira
Nogueira,Rita Maria Ribeiro
Edgil,Dianna
Schatzmayr,Hermann Gonçalves
Riley,Lee W
Harris,Eva
author_role author
author2 Miagostovich,Marize Pereira
Nogueira,Rita Maria Ribeiro
Edgil,Dianna
Schatzmayr,Hermann Gonçalves
Riley,Lee W
Harris,Eva
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Santos,Flávia Barreto dos
Miagostovich,Marize Pereira
Nogueira,Rita Maria Ribeiro
Edgil,Dianna
Schatzmayr,Hermann Gonçalves
Riley,Lee W
Harris,Eva
dc.subject.por.fl_str_mv dengue virus type 2
complete genome
Brazil
topic dengue virus type 2
complete genome
Brazil
dc.description.none.fl_txt_mv In the last decade, dengue fever (DF) in Brazil has been recognized as an important public health problem, and an increasing number of dengue haemorrhagic fever (DHF) cases have been reported since the introduction of dengue virus type 2 (DEN-2) into the country in 1990. In order to analyze the complete genome sequence of a DEN-2 Brazilian strain (BR64022/98), we designed primers to amplify contiguous segments of approximately 500 base pairs across the entire sequence of the viral genome. Twenty fragments amplified by reverse transcriptase-PCR were cloned, and the complete nucleotide and the deduced amino acid sequences were determined. This constitutes the first complete genetic characterization of a DEN-2 strain from Brazil. All amino acid changes differentiating strains related to the Asian/American-Asian genotype were observed in BR64022/98, indicating the Asiatic origin of the strain.
description In the last decade, dengue fever (DF) in Brazil has been recognized as an important public health problem, and an increasing number of dengue haemorrhagic fever (DHF) cases have been reported since the introduction of dengue virus type 2 (DEN-2) into the country in 1990. In order to analyze the complete genome sequence of a DEN-2 Brazilian strain (BR64022/98), we designed primers to amplify contiguous segments of approximately 500 base pairs across the entire sequence of the viral genome. Twenty fragments amplified by reverse transcriptase-PCR were cloned, and the complete nucleotide and the deduced amino acid sequences were determined. This constitutes the first complete genetic characterization of a DEN-2 strain from Brazil. All amino acid changes differentiating strains related to the Asian/American-Asian genotype were observed in BR64022/98, indicating the Asiatic origin of the strain.
publishDate 2002
dc.date.none.fl_str_mv 2002-10-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000700011
url http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0074-02762002000700011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0074-02762002000700011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
publisher.none.fl_str_mv Instituto Oswaldo Cruz, Ministério da Saúde
dc.source.none.fl_str_mv Memórias do Instituto Oswaldo Cruz v.97 n.7 2002
reponame:Memórias do Instituto Oswaldo Cruz
instname:Fundação Oswaldo Cruz
instacron:FIOCRUZ
reponame_str Memórias do Instituto Oswaldo Cruz
collection Memórias do Instituto Oswaldo Cruz
instname_str Fundação Oswaldo Cruz
instacron_str FIOCRUZ
institution FIOCRUZ
repository.name.fl_str_mv Memórias do Instituto Oswaldo Cruz - Fundação Oswaldo Cruz
repository.mail.fl_str_mv
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