Dengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivo
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2013 |
| Outros Autores: | , , , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Repositório Institucional da FIOCRUZ (ARCA) |
| Texto Completo: | https://www.arca.fiocruz.br/handle/icict/11773 |
Resumo: | Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. |
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Gandini, MarianaGras, ChristopherAzeredo, Elzinandes LealPinto, Luzia Maria de OliveiraSmith, NikaiaDespres, PhilippeCunha, Rivaldo Venâncio daSouza, Luiz José deKubelka, Claire FernandesHerbeuva, Jean-Philippe2015-09-21T17:25:45Z2015-09-21T17:25:45Z2013GANDINI, Mariana; et al. Dengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivo. PLoS Negl Trop Dis, v.7, n.6, e2257, 14p, June 2013.10.1371/journal.pntd.0002257https://www.arca.fiocruz.br/handle/icict/11773engPlos OneDengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivoinfo:eu-repo/semantics/publishedVersioninfo:eu-repo/semantics/articleFundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Université Paris Descartes. CNRS UMR 8147. Paris, Frrance.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Université Paris Descartes. CNRS UMR 8601. Chimie et Biologie, Nucléo(s)tides et Immunologie Thérapeutique (CBNIT). Paris, France.Institut Pasteur. Unité des Interactions moléculaires Flavivirus-Hôtes. Paris, FranceUniversidade Federal do Mato Grosso do Sul. Faculdade de Medicina. Departamento de Clínica Medica. Campo Grande, MS, Brasil.Centro de Referencia em Dengue.Campos de Goytacases, RJ, Brasil.Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil.Université Paris Descartes. CNRS UMR 8601. Chimie et Biologie, Nucléo(s)tides et Immunologie Thérapeutique (CBNIT). Paris, France.Background: Dengue displays a broad spectrum of clinical manifestations that may vary from asymptomatic to severe and even fatal features. Plasma leakage/hemorrhages can be caused by a cytokine storm induced by monocytes and dendritic cells during dengue virus (DENV) replication. Plasmacytoid dendritic cells (pDCs) are innate immune cells and in response to virus exposure secrete IFN-a and express membrane TRAIL (mTRAIL). We aimed to characterize pDC activation in dengue patients and their function under DENV-2 stimulation in vitro. Methods & Findings: Flow cytometry analysis (FCA) revealed that pDCs of mild dengue patients exhibit significantly higher frequencies of mTRAIL compared to severe cases or healthy controls. Plasma levels of IFN-a and soluble TRAIL are increased in mild compared to severe dengue patients, positively correlating with pDC activation. FCA experiments showed that in vitro exposure to DENV-2 induced mTRAIL expression on pDC. Furthermore, three dimension microscopy highlighted that TRAIL was relocalized from intracellular compartment to plasma membrane. Chloroquine treatment inhibited DENV-2- induced mTRAIL relocalization and IFN-a production by pDC. Endosomal viral degradation blockade by chloroquine allowed viral antigens detection inside pDCs. All those data are in favor of endocytosis pathway activation by DENV-2 in pDC. Coculture of pDC/DENV-2-infected monocytes revealed a dramatic decrease of antigen detection by FCA. This viral antigens reduction in monocytes was also observed after exogenous IFN-a treatment. Thus, pDC effect on viral load reduction was mainly dependent on IFN-a production Conclusions: This investigation characterizes, during DENV-2 infection, activation of pDCs in vivo and their antiviral role in vitro. Thus, we propose TRAIL-expressing pDCs may have an important role in the outcome of disease.DenguePlasmacytoid Dendritic CellsIn VitroMonocitesDengueCélulas DendríticasTécnicas In VitroMonócitosinfo:eu-repo/semantics/openAccessreponame:Repositório Institucional da FIOCRUZ (ARCA)instname:Fundação Oswaldo Cruz (FIOCRUZ)instacron:FIOCRUZLICENSElicense.txttext/plain1914https://www.arca.fiocruz.br/bitstream/icict/11773/1/license.txt7d48279ffeed55da8dfe2f8e81f3b81fMD51ORIGINALmariana_gandini_etal_IOC_2013.pdfapplication/pdf7291079https://www.arca.fiocruz.br/bitstream/icict/11773/2/mariana_gandini_etal_IOC_2013.pdf4af4061537bbe91939f7ce093e2ac42aMD52TEXTmariana_gandini_etal_IOC_2013.pdf.txtmariana_gandini_etal_IOC_2013.pdf.txtExtracted texttext/plain70514https://www.arca.fiocruz.br/bitstream/icict/11773/3/mariana_gandini_etal_IOC_2013.pdf.txta8a91757d4a8d4b7cf066bfe04259591MD53icict/117732022-06-24 13:03:07.77oai:www.arca.fiocruz.br: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ório InstitucionalPUBhttps://www.arca.fiocruz.br/oai/requestrepositorio.arca@fiocruz.bropendoar:21352022-06-24T16:03:07Repositório Institucional da FIOCRUZ (ARCA) - Fundação Oswaldo Cruz (FIOCRUZ)false |
| dc.title.pt_BR.fl_str_mv |
Dengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivo |
| title |
Dengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivo |
| spellingShingle |
Dengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivo Gandini, Mariana Dengue Plasmacytoid Dendritic Cells In Vitro Monocites Dengue Células Dendríticas Técnicas In Vitro Monócitos |
| title_short |
Dengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivo |
| title_full |
Dengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivo |
| title_fullStr |
Dengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivo |
| title_full_unstemmed |
Dengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivo |
| title_sort |
Dengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivo |
| author |
Gandini, Mariana |
| author_facet |
Gandini, Mariana Gras, Christopher Azeredo, Elzinandes Leal Pinto, Luzia Maria de Oliveira Smith, Nikaia Despres, Philippe Cunha, Rivaldo Venâncio da Souza, Luiz José de Kubelka, Claire Fernandes Herbeuva, Jean-Philippe |
| author_role |
author |
| author2 |
Gras, Christopher Azeredo, Elzinandes Leal Pinto, Luzia Maria de Oliveira Smith, Nikaia Despres, Philippe Cunha, Rivaldo Venâncio da Souza, Luiz José de Kubelka, Claire Fernandes Herbeuva, Jean-Philippe |
| author2_role |
author author author author author author author author author |
| dc.contributor.author.fl_str_mv |
Gandini, Mariana Gras, Christopher Azeredo, Elzinandes Leal Pinto, Luzia Maria de Oliveira Smith, Nikaia Despres, Philippe Cunha, Rivaldo Venâncio da Souza, Luiz José de Kubelka, Claire Fernandes Herbeuva, Jean-Philippe |
| dc.subject.en.pt_BR.fl_str_mv |
Dengue Plasmacytoid Dendritic Cells In Vitro Monocites |
| topic |
Dengue Plasmacytoid Dendritic Cells In Vitro Monocites Dengue Células Dendríticas Técnicas In Vitro Monócitos |
| dc.subject.decs.pt_BR.fl_str_mv |
Dengue Células Dendríticas Técnicas In Vitro Monócitos |
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Fundação Oswaldo Cruz. Instituto Oswaldo Cruz. Laboratório de Imunologia Viral. Rio de Janeiro, RJ, Brasil. |
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2013 |
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2013 |
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2015-09-21T17:25:45Z |
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2015-09-21T17:25:45Z |
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info:eu-repo/semantics/publishedVersion |
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info:eu-repo/semantics/article |
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article |
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publishedVersion |
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GANDINI, Mariana; et al. Dengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivo. PLoS Negl Trop Dis, v.7, n.6, e2257, 14p, June 2013. |
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https://www.arca.fiocruz.br/handle/icict/11773 |
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10.1371/journal.pntd.0002257 |
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GANDINI, Mariana; et al. Dengue Virus Activates Membrane TRAIL Relocalization and IFN-a Production by Human Plasmacytoid Dendritic Cells In Vitro and In Vivo. PLoS Negl Trop Dis, v.7, n.6, e2257, 14p, June 2013. 10.1371/journal.pntd.0002257 |
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Plos One |
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